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Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda).
Cell Tissue Res. 2005 Feb; 319(2):331-9.CT

Abstract

The ontogeny of osmoregulation was examined in the branchial cavity of embryonic and early post-embryonic stages of the crayfish Astacus leptodactylus maintained in freshwater, at the sub-cellular level through the detection of the sodium-potassium adenosine triphosphatase (Na(+),K(+)-ATPase). The embryonic rate of development was calculated according to the eye index (EI) which was 430-450 microm at hatching. The distribution of the enzyme was identified by immunofluorescence microscopy using a monoclonal antibody IgGalpha5 raised against the avian alpha-subunit of the Na(+),K(+)-ATPase. Immunoreactivity staining, indicating the presence of Na(+), K(+)-ATPase appeared in the gills of late embryos (EI>/=400 microm), i.e. a few days before hatching time, and steadily increased throughout the late embryonic and early post-embryonic development. The appearance of the enzyme correlates with the ability to osmoregulate which also occurs late in the embryonic development at EI 410-420 microm and with tissue differentiation within the gill filaments. These observations indicate that the physiological shift from osmoconforming embryos to hyper-regulating late embryos and post-hatching stages in freshwater must originate partly from the differentiation in the gill epithelia of ionocytes which are the site of ion pumping, as suggested by the location of Na(+),K(+)-ATPase. Only the gills were immunostained and a lack of specific staining was noted in the lamina and the branchiostegites. Therefore, osmoregulation through Na(+)active uptake is likely achieved in embryos at the gill level; all the newly formed gills in embryos function in ion regulation; other parts of the branchial chamber such as the branchiostegites and lamina do not appear to be involved in osmoregulation.

Authors+Show Affiliations

Centre d'Ecologie et de Physiologie Energétiques CEPE-CNRS, UPR 9010, 23 Rue Becquerel, 67087 Strasbourg, France. j-h.lignot@c-strasbourg.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15592941

Citation

Lignot, Jean-Hervé, et al. "Immunolocalization of Na+,K+-ATPase in the Branchial Cavity During the Early Development of the Crayfish Astacus Leptodactylus (Crustacea, Decapoda)." Cell and Tissue Research, vol. 319, no. 2, 2005, pp. 331-9.
Lignot JH, Susanto GN, Charmantier-Daures M, et al. Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda). Cell Tissue Res. 2005;319(2):331-9.
Lignot, J. H., Susanto, G. N., Charmantier-Daures, M., & Charmantier, G. (2005). Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda). Cell and Tissue Research, 319(2), 331-9.
Lignot JH, et al. Immunolocalization of Na+,K+-ATPase in the Branchial Cavity During the Early Development of the Crayfish Astacus Leptodactylus (Crustacea, Decapoda). Cell Tissue Res. 2005;319(2):331-9. PubMed PMID: 15592941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda). AU - Lignot,Jean-Hervé, AU - Susanto,Gregorius Nugroho, AU - Charmantier-Daures,Mireille, AU - Charmantier,Guy, Y1 - 2004/12/11/ PY - 2004/07/27/received PY - 2004/10/05/accepted PY - 2004/12/14/pubmed PY - 2005/6/7/medline PY - 2004/12/14/entrez SP - 331 EP - 9 JF - Cell and tissue research JO - Cell Tissue Res. VL - 319 IS - 2 N2 - The ontogeny of osmoregulation was examined in the branchial cavity of embryonic and early post-embryonic stages of the crayfish Astacus leptodactylus maintained in freshwater, at the sub-cellular level through the detection of the sodium-potassium adenosine triphosphatase (Na(+),K(+)-ATPase). The embryonic rate of development was calculated according to the eye index (EI) which was 430-450 microm at hatching. The distribution of the enzyme was identified by immunofluorescence microscopy using a monoclonal antibody IgGalpha5 raised against the avian alpha-subunit of the Na(+),K(+)-ATPase. Immunoreactivity staining, indicating the presence of Na(+), K(+)-ATPase appeared in the gills of late embryos (EI>/=400 microm), i.e. a few days before hatching time, and steadily increased throughout the late embryonic and early post-embryonic development. The appearance of the enzyme correlates with the ability to osmoregulate which also occurs late in the embryonic development at EI 410-420 microm and with tissue differentiation within the gill filaments. These observations indicate that the physiological shift from osmoconforming embryos to hyper-regulating late embryos and post-hatching stages in freshwater must originate partly from the differentiation in the gill epithelia of ionocytes which are the site of ion pumping, as suggested by the location of Na(+),K(+)-ATPase. Only the gills were immunostained and a lack of specific staining was noted in the lamina and the branchiostegites. Therefore, osmoregulation through Na(+)active uptake is likely achieved in embryos at the gill level; all the newly formed gills in embryos function in ion regulation; other parts of the branchial chamber such as the branchiostegites and lamina do not appear to be involved in osmoregulation. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/15592941/Immunolocalization_of_Na+K+_ATPase_in_the_branchial_cavity_during_the_early_development_of_the_crayfish_Astacus_leptodactylus__Crustacea_Decapoda__ L2 - https://dx.doi.org/10.1007/s00441-004-1015-2 DB - PRIME DP - Unbound Medicine ER -