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Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees.
Phytochem Anal. 2004 Nov-Dec; 15(6):365-71.PA

Abstract

Simple and rapid methods have been developed for the extraction and simultaneous determination of the three active diterpenoids, andrographolide (AP1), 14-deoxy-11,12-didehydroandrographolide (AP3) and neoandrographolide (AP4), from various samples of Andrographis paniculata (Burm.f.) Nees. Methanol extracts from the dried leaves, stems and crude products were analysed by isocratic HPLC using a methanol and water mobile phase with monitoring at 220 nm. There was a large variation of the three active diterpenoids in different A. paniculata products obtained from Thai markets. The results indicated that the amounts of these active compounds consumed, based on the recommended daily doses, from materials obtained from the different suppliers will be different. In addition, the stability of these three active compounds was also examined in dry herbs stored at room temperature. The results showed that andrographolide was more stable than the others. In contrast, the content of 14-deoxy-11,12-didehydroandrographolide increased and the neoandrographolide content fluctuated during storage time. The combination of different levels of these compounds in the source materials and the changes during storage could have a significant effect on the efficacy of this traditional herbal medicine in clinical treatment.

Authors+Show Affiliations

Pholphana N
Laboratory of Pharmacology, Chulabhorn Research Institute, Vibhavadee-Rangsit Highway, Laksi, Bangkok 10210, Thailand.
Rangkadilok N
No affiliation info available
Thongnest S
No affiliation info available
Ruchirawat S
No affiliation info available
Ruchirawat M
No affiliation info available
Satayavivad J
No affiliation info available

MeSH

AndrographisChromatography, High Pressure LiquidDiterpenesDrug StorageGlucosidesMolecular StructurePlant ExtractsPlant PreparationsTetrahydronaphthalenesTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15595451

Citation

Pholphana, Nanthanit, et al. "Determination and Variation of Three Active Diterpenoids in Andrographis Paniculata (Burm.f.) Nees." Phytochemical Analysis : PCA, vol. 15, no. 6, 2004, pp. 365-71.
Pholphana N, Rangkadilok N, Thongnest S, et al. Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees. Phytochem Anal. 2004;15(6):365-71.
Pholphana, N., Rangkadilok, N., Thongnest, S., Ruchirawat, S., Ruchirawat, M., & Satayavivad, J. (2004). Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees. Phytochemical Analysis : PCA, 15(6), 365-71.
Pholphana N, et al. Determination and Variation of Three Active Diterpenoids in Andrographis Paniculata (Burm.f.) Nees. Phytochem Anal. 2004 Nov-Dec;15(6):365-71. PubMed PMID: 15595451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination and variation of three active diterpenoids in Andrographis paniculata (Burm.f.) Nees. AU - Pholphana,Nanthanit, AU - Rangkadilok,Nuchanart, AU - Thongnest,Sanit, AU - Ruchirawat,Somsak, AU - Ruchirawat,Mathuros, AU - Satayavivad,Jutamaad, PY - 2004/12/15/pubmed PY - 2005/2/3/medline PY - 2004/12/15/entrez SP - 365 EP - 71 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 15 IS - 6 N2 - Simple and rapid methods have been developed for the extraction and simultaneous determination of the three active diterpenoids, andrographolide (AP1), 14-deoxy-11,12-didehydroandrographolide (AP3) and neoandrographolide (AP4), from various samples of Andrographis paniculata (Burm.f.) Nees. Methanol extracts from the dried leaves, stems and crude products were analysed by isocratic HPLC using a methanol and water mobile phase with monitoring at 220 nm. There was a large variation of the three active diterpenoids in different A. paniculata products obtained from Thai markets. The results indicated that the amounts of these active compounds consumed, based on the recommended daily doses, from materials obtained from the different suppliers will be different. In addition, the stability of these three active compounds was also examined in dry herbs stored at room temperature. The results showed that andrographolide was more stable than the others. In contrast, the content of 14-deoxy-11,12-didehydroandrographolide increased and the neoandrographolide content fluctuated during storage time. The combination of different levels of these compounds in the source materials and the changes during storage could have a significant effect on the efficacy of this traditional herbal medicine in clinical treatment. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/15595451/Determination_and_variation_of_three_active_diterpenoids_in_Andrographis_paniculata__Burm_f___Nees_ DB - PRIME DP - Unbound Medicine ER -