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Telomerase activation by Epstein-Barr virus latent membrane protein 1 is associated with c-Myc expression in human nasopharyngeal epithelial cells.
J Exp Clin Cancer Res. 2004 Sep; 23(3):495-506.JE

Abstract

Latent membrane protein 1 (LMP1), one of the oncoproteins encoded by Epstein-Barr virus is sufficient for the development of nasopharyngeal carcinoma in vivo and nasopharyngeal epithelial cellular immortalization in vitro. It has also been shown to increase the telomerase activity in primary human nasopharyngeal epithelial cells by an unknown mechanism. We reported here that LMP1 could increase telomerase activity in coordination with LMP1-induced c-Myc expression in LMP1-transfected primary human nasopharyngeal epithelial cells or in a dual-stable LMP1 integrated nasopharyngeal carcinoma cell line with Tet-on regulatory system, named Tet-on-LMP1 HNE2 by PCR-ELISA analysis and reporter gene assay. Blocking of LMP1 expression decreased telomerase activity and c-myc transactivation. Mutagenesis of Myc-responsive E-box elements in the minimal core of hTERT promoter could inhibit the hTERT expression induced by LMP1. Moreover, blocking of c-myc transactivation could further decrease LMP1-mediated hTERT expression. It has been suggested that LMP1 can be used to aid myc control telomerase. In addition, we also found that C-terminus of LMP1, including CTAR1 and CTAR2 domains participated in telomerase activation. Together, these findings suggested that LMP1 activated telomerase via c-myc.

Authors+Show Affiliations

Dept of Pathology and laboratory Medicine, University of South Carolina, Charleston, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15595642

Citation

Yang, J, et al. "Telomerase Activation By Epstein-Barr Virus Latent Membrane Protein 1 Is Associated With c-Myc Expression in Human Nasopharyngeal Epithelial Cells." Journal of Experimental & Clinical Cancer Research : CR, vol. 23, no. 3, 2004, pp. 495-506.
Yang J, Deng X, Deng L, et al. Telomerase activation by Epstein-Barr virus latent membrane protein 1 is associated with c-Myc expression in human nasopharyngeal epithelial cells. J Exp Clin Cancer Res. 2004;23(3):495-506.
Yang, J., Deng, X., Deng, L., Gu, H., Fan, W., & Cao, Y. (2004). Telomerase activation by Epstein-Barr virus latent membrane protein 1 is associated with c-Myc expression in human nasopharyngeal epithelial cells. Journal of Experimental & Clinical Cancer Research : CR, 23(3), 495-506.
Yang J, et al. Telomerase Activation By Epstein-Barr Virus Latent Membrane Protein 1 Is Associated With c-Myc Expression in Human Nasopharyngeal Epithelial Cells. J Exp Clin Cancer Res. 2004;23(3):495-506. PubMed PMID: 15595642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase activation by Epstein-Barr virus latent membrane protein 1 is associated with c-Myc expression in human nasopharyngeal epithelial cells. AU - Yang,J, AU - Deng,X, AU - Deng,L, AU - Gu,H, AU - Fan,W, AU - Cao,Y, PY - 2004/12/15/pubmed PY - 2005/4/19/medline PY - 2004/12/15/entrez SP - 495 EP - 506 JF - Journal of experimental & clinical cancer research : CR JO - J Exp Clin Cancer Res VL - 23 IS - 3 N2 - Latent membrane protein 1 (LMP1), one of the oncoproteins encoded by Epstein-Barr virus is sufficient for the development of nasopharyngeal carcinoma in vivo and nasopharyngeal epithelial cellular immortalization in vitro. It has also been shown to increase the telomerase activity in primary human nasopharyngeal epithelial cells by an unknown mechanism. We reported here that LMP1 could increase telomerase activity in coordination with LMP1-induced c-Myc expression in LMP1-transfected primary human nasopharyngeal epithelial cells or in a dual-stable LMP1 integrated nasopharyngeal carcinoma cell line with Tet-on regulatory system, named Tet-on-LMP1 HNE2 by PCR-ELISA analysis and reporter gene assay. Blocking of LMP1 expression decreased telomerase activity and c-myc transactivation. Mutagenesis of Myc-responsive E-box elements in the minimal core of hTERT promoter could inhibit the hTERT expression induced by LMP1. Moreover, blocking of c-myc transactivation could further decrease LMP1-mediated hTERT expression. It has been suggested that LMP1 can be used to aid myc control telomerase. In addition, we also found that C-terminus of LMP1, including CTAR1 and CTAR2 domains participated in telomerase activation. Together, these findings suggested that LMP1 activated telomerase via c-myc. SN - 0392-9078 UR - https://www.unboundmedicine.com/medline/citation/15595642/Telomerase_activation_by_Epstein_Barr_virus_latent_membrane_protein_1_is_associated_with_c_Myc_expression_in_human_nasopharyngeal_epithelial_cells_ L2 - https://antibodies.cancer.gov/detail/CPTC-OTUB1-1 DB - PRIME DP - Unbound Medicine ER -