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Diethylstilbestrol exposure during fetal development affects thymus: studies in fourteen-month-old mice.
J Reprod Immunol. 2004 Dec; 64(1-2):75-90.JR

Abstract

In utero exposure to diethylstilbestrol (DES) may have long-term immunological alterations after birth. It is hypothesized that in utero exposure to DES may pre-program the thymus to result in aberrant response to a subsequent adult exposure to an endocrine disrupting chemical. Pregnant mice at 14-days gestation were given either DES (0.25 microg; DESprenatal) or vehicle oil (Oil; Oil(prenatal)). One-year after birth, these mice were given a single dose of DES (DESadult) and thymii of these mice were studied two months later. DESprenatal/DESadult female mice had a significant decrease in thymocyte cellularity compared to female controls (Oil(prenatal)/DESadult). In contrast, male DESprenatal/DESadult mice had increased thymic mass and a trend towards increased thymocyte cellularity. There were no significant differences in the relative percentages of major thymocyte subsets, CD4-CD8-, CD4+CD8+, CD4+CD8-, CD4-CD8+, in either female or male DESprenatal/DESadult mice compared to their sex-matched controls. Nevertheless, thymocytes cultured in media alone showed increased percentage of apoptosis in CD4+CD8+ subset from female DESprenatal/DESadult mice compared to similar cultures from sex-matched controls. Interestingly, the percentage of apoptosis of CD4+CD8+ thymocytes in media-only cultures from DESprenatal/DESadult female mice was comparable to in vitro dexamethasone-exposed cultures from Oil(prenatal)/DESadult female mice. This pattern of increased apoptosis of female CD4+CD8+ subset was not noticed in male DESprenatal/DESadult mice. This implies that prenatal DES exposure in female mice intrinsically alters the degree of apoptosis in CD4+CD8+ thymocyte subset. Together, these data imply that prenatal DES exposure induces long-term thymic changes in a sex-related fashion.

Authors+Show Affiliations

Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24060-0342, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15596228

Citation

Fenaux, J B., et al. "Diethylstilbestrol Exposure During Fetal Development Affects Thymus: Studies in Fourteen-month-old Mice." Journal of Reproductive Immunology, vol. 64, no. 1-2, 2004, pp. 75-90.
Fenaux JB, Gogal RM, Ahmed SA. Diethylstilbestrol exposure during fetal development affects thymus: studies in fourteen-month-old mice. J Reprod Immunol. 2004;64(1-2):75-90.
Fenaux, J. B., Gogal, R. M., & Ahmed, S. A. (2004). Diethylstilbestrol exposure during fetal development affects thymus: studies in fourteen-month-old mice. Journal of Reproductive Immunology, 64(1-2), 75-90.
Fenaux JB, Gogal RM, Ahmed SA. Diethylstilbestrol Exposure During Fetal Development Affects Thymus: Studies in Fourteen-month-old Mice. J Reprod Immunol. 2004;64(1-2):75-90. PubMed PMID: 15596228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diethylstilbestrol exposure during fetal development affects thymus: studies in fourteen-month-old mice. AU - Fenaux,J B, AU - Gogal,R M,Jr AU - Ahmed,S Ansar, PY - 2004/08/20/revised PY - 2004/08/23/accepted PY - 2004/12/15/pubmed PY - 2005/6/10/medline PY - 2004/12/15/entrez SP - 75 EP - 90 JF - Journal of reproductive immunology JO - J Reprod Immunol VL - 64 IS - 1-2 N2 - In utero exposure to diethylstilbestrol (DES) may have long-term immunological alterations after birth. It is hypothesized that in utero exposure to DES may pre-program the thymus to result in aberrant response to a subsequent adult exposure to an endocrine disrupting chemical. Pregnant mice at 14-days gestation were given either DES (0.25 microg; DESprenatal) or vehicle oil (Oil; Oil(prenatal)). One-year after birth, these mice were given a single dose of DES (DESadult) and thymii of these mice were studied two months later. DESprenatal/DESadult female mice had a significant decrease in thymocyte cellularity compared to female controls (Oil(prenatal)/DESadult). In contrast, male DESprenatal/DESadult mice had increased thymic mass and a trend towards increased thymocyte cellularity. There were no significant differences in the relative percentages of major thymocyte subsets, CD4-CD8-, CD4+CD8+, CD4+CD8-, CD4-CD8+, in either female or male DESprenatal/DESadult mice compared to their sex-matched controls. Nevertheless, thymocytes cultured in media alone showed increased percentage of apoptosis in CD4+CD8+ subset from female DESprenatal/DESadult mice compared to similar cultures from sex-matched controls. Interestingly, the percentage of apoptosis of CD4+CD8+ thymocytes in media-only cultures from DESprenatal/DESadult female mice was comparable to in vitro dexamethasone-exposed cultures from Oil(prenatal)/DESadult female mice. This pattern of increased apoptosis of female CD4+CD8+ subset was not noticed in male DESprenatal/DESadult mice. This implies that prenatal DES exposure in female mice intrinsically alters the degree of apoptosis in CD4+CD8+ thymocyte subset. Together, these data imply that prenatal DES exposure induces long-term thymic changes in a sex-related fashion. SN - 0165-0378 UR - https://www.unboundmedicine.com/medline/citation/15596228/Diethylstilbestrol_exposure_during_fetal_development_affects_thymus:_studies_in_fourteen_month_old_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0378(04)00139-1 DB - PRIME DP - Unbound Medicine ER -