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Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats.
Toxicology. 2005 Feb 14; 207(2):169-77.T

Abstract

The toxicity of gentamicin (GEN) in the kidney seems to relate to the generation of reactive oxygen species (ROS). Caffeic acid phenethyl ester (CAPE) has been demonstrated to have antioxidant, free radical scavenger and anti-inflammatory effects. It has been proposed that antioxidant maintain the concentration of reduced glutathione (GSH) may restore the cellular defense mechanisms and block lipid peroxidation thus protect against the toxicity of wide variety of nephrotoxic chemicals. We investigated the effects of CAPE on GEN-induced changes in renal malondialdehyde (MDA), a lipid peroxidation product, nitric oxide (NO) generation, superoxide dismutase (SOD), catalase (CAT) activities, GSH content, blood urea nitrogen (BUN) and serum creatinine (Cr) levels. Morphological changes in the kidney were also examined. A total of 32 rats were equally divided into four groups which were: (1) control, (2) injected with intraperitoneally (i.p.) GEN, (3) injected with i.p. GEN+CAPE and (4) injected with i.p. CAPE. GEN administration to control rats increased renal MDA and NO generation but decreased SOD and CAT activities, and GSH content. CAPE administration with GEN injections caused significantly decreased MDA, NO generation and increased SOD, CAT activities and GSH content when compared with GEN alone. Serum level of BUN and Cr significantly increased as a result of nephrotoxicity. CAPE also, significantly decreased serum BUN and Cr levels. Morphological changes in the kidney due to GEN, including tubular necrosis, were evaluated qualitatively. In addition, CAPE reduced the degree of kidney tissue damage induced by GEN. Both biochemical findings and histopathological evidence showed that administration of CAPE reduced the GEN-induced kidney damage. Our results indicated that CAPE acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of GEN both at the biochemical and histological level. Thus, CAPE could be effectively combined with GEN treatment.

Authors+Show Affiliations

Department of Pharmacology, Faculty of Medicine, Inonu University, 44280 Malatya, Turkey. hparlakpinar@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15596248

Citation

Parlakpinar, H, et al. "Protective Role of Caffeic Acid Phenethyl Ester (cape) On Gentamicin-induced Acute Renal Toxicity in Rats." Toxicology, vol. 207, no. 2, 2005, pp. 169-77.
Parlakpinar H, Tasdemir S, Polat A, et al. Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats. Toxicology. 2005;207(2):169-77.
Parlakpinar, H., Tasdemir, S., Polat, A., Bay-Karabulut, A., Vardi, N., Ucar, M., & Acet, A. (2005). Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats. Toxicology, 207(2), 169-77.
Parlakpinar H, et al. Protective Role of Caffeic Acid Phenethyl Ester (cape) On Gentamicin-induced Acute Renal Toxicity in Rats. Toxicology. 2005 Feb 14;207(2):169-77. PubMed PMID: 15596248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective role of caffeic acid phenethyl ester (cape) on gentamicin-induced acute renal toxicity in rats. AU - Parlakpinar,H, AU - Tasdemir,S, AU - Polat,A, AU - Bay-Karabulut,A, AU - Vardi,N, AU - Ucar,M, AU - Acet,A, PY - 2004/07/22/received PY - 2004/08/14/revised PY - 2004/08/14/accepted PY - 2004/12/15/pubmed PY - 2005/3/22/medline PY - 2004/12/15/entrez SP - 169 EP - 77 JF - Toxicology JO - Toxicology VL - 207 IS - 2 N2 - The toxicity of gentamicin (GEN) in the kidney seems to relate to the generation of reactive oxygen species (ROS). Caffeic acid phenethyl ester (CAPE) has been demonstrated to have antioxidant, free radical scavenger and anti-inflammatory effects. It has been proposed that antioxidant maintain the concentration of reduced glutathione (GSH) may restore the cellular defense mechanisms and block lipid peroxidation thus protect against the toxicity of wide variety of nephrotoxic chemicals. We investigated the effects of CAPE on GEN-induced changes in renal malondialdehyde (MDA), a lipid peroxidation product, nitric oxide (NO) generation, superoxide dismutase (SOD), catalase (CAT) activities, GSH content, blood urea nitrogen (BUN) and serum creatinine (Cr) levels. Morphological changes in the kidney were also examined. A total of 32 rats were equally divided into four groups which were: (1) control, (2) injected with intraperitoneally (i.p.) GEN, (3) injected with i.p. GEN+CAPE and (4) injected with i.p. CAPE. GEN administration to control rats increased renal MDA and NO generation but decreased SOD and CAT activities, and GSH content. CAPE administration with GEN injections caused significantly decreased MDA, NO generation and increased SOD, CAT activities and GSH content when compared with GEN alone. Serum level of BUN and Cr significantly increased as a result of nephrotoxicity. CAPE also, significantly decreased serum BUN and Cr levels. Morphological changes in the kidney due to GEN, including tubular necrosis, were evaluated qualitatively. In addition, CAPE reduced the degree of kidney tissue damage induced by GEN. Both biochemical findings and histopathological evidence showed that administration of CAPE reduced the GEN-induced kidney damage. Our results indicated that CAPE acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of GEN both at the biochemical and histological level. Thus, CAPE could be effectively combined with GEN treatment. SN - 0300-483X UR - https://www.unboundmedicine.com/medline/citation/15596248/Protective_role_of_caffeic_acid_phenethyl_ester__cape__on_gentamicin_induced_acute_renal_toxicity_in_rats_ DB - PRIME DP - Unbound Medicine ER -