Tags

Type your tag names separated by a space and hit enter

EGF, EGF receptor, basic FGF, TGF beta-1, and IL-1 alpha mRNA in human corneal epithelial cells and stromal fibroblasts.
Invest Ophthalmol Vis Sci. 1992 Apr; 33(5):1756-65.IO

Abstract

We sought to determine whether human corneal epithelial cells and stromal fibroblasts synthesize messenger RNAs (mRNA) coding for epidermal growth factor (EGF), EGF receptor, basic fibroblast growth factor (basic FGF), transforming growth factor-beta 1 (TGF-beta 1), and interleukin-1 alpha (IL-1 alpha). Total cellular RNA was extracted from cultured stromal fibroblasts and ex vivo and cultured corneal epithelial cells. Oligo dt-primed complementary DNA (cDNA) was synthesized from each RNA sample. The polymerase chain reaction (PCR) was used to amplify sequences for EGF, EGF receptor, basic FGF, TGF-beta 1, IL-1 alpha, and beta actin from cDNA samples from each cell type. Southern blots of the PCR products were probed with oligonucleotides complementary to internal sequences within each of the amplified products. The amplification products were shown to be specific. For each modulator, the amplification product of the expected size was identified with at least one specific, alternative amplification product. The alternative splicing products suggest that there may be alternative mRNA splicing for each of the modulators studied. Differences were noted in the IL-1 alpha specific amplification products in stromal fibroblasts compared to corneal epithelial cells. EGF and EGF receptor mRNA production in human corneal epithelial cells and stromal fibroblasts suggest an autocrine role for EGF in the physiology of each of these cell types.

Authors+Show Affiliations

Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1559775

Citation

Wilson, S E., et al. "EGF, EGF Receptor, Basic FGF, TGF Beta-1, and IL-1 Alpha mRNA in Human Corneal Epithelial Cells and Stromal Fibroblasts." Investigative Ophthalmology & Visual Science, vol. 33, no. 5, 1992, pp. 1756-65.
Wilson SE, He YG, Lloyd SA. EGF, EGF receptor, basic FGF, TGF beta-1, and IL-1 alpha mRNA in human corneal epithelial cells and stromal fibroblasts. Invest Ophthalmol Vis Sci. 1992;33(5):1756-65.
Wilson, S. E., He, Y. G., & Lloyd, S. A. (1992). EGF, EGF receptor, basic FGF, TGF beta-1, and IL-1 alpha mRNA in human corneal epithelial cells and stromal fibroblasts. Investigative Ophthalmology & Visual Science, 33(5), 1756-65.
Wilson SE, He YG, Lloyd SA. EGF, EGF Receptor, Basic FGF, TGF Beta-1, and IL-1 Alpha mRNA in Human Corneal Epithelial Cells and Stromal Fibroblasts. Invest Ophthalmol Vis Sci. 1992;33(5):1756-65. PubMed PMID: 1559775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EGF, EGF receptor, basic FGF, TGF beta-1, and IL-1 alpha mRNA in human corneal epithelial cells and stromal fibroblasts. AU - Wilson,S E, AU - He,Y G, AU - Lloyd,S A, PY - 1992/4/1/pubmed PY - 1992/4/1/medline PY - 1992/4/1/entrez SP - 1756 EP - 65 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 33 IS - 5 N2 - We sought to determine whether human corneal epithelial cells and stromal fibroblasts synthesize messenger RNAs (mRNA) coding for epidermal growth factor (EGF), EGF receptor, basic fibroblast growth factor (basic FGF), transforming growth factor-beta 1 (TGF-beta 1), and interleukin-1 alpha (IL-1 alpha). Total cellular RNA was extracted from cultured stromal fibroblasts and ex vivo and cultured corneal epithelial cells. Oligo dt-primed complementary DNA (cDNA) was synthesized from each RNA sample. The polymerase chain reaction (PCR) was used to amplify sequences for EGF, EGF receptor, basic FGF, TGF-beta 1, IL-1 alpha, and beta actin from cDNA samples from each cell type. Southern blots of the PCR products were probed with oligonucleotides complementary to internal sequences within each of the amplified products. The amplification products were shown to be specific. For each modulator, the amplification product of the expected size was identified with at least one specific, alternative amplification product. The alternative splicing products suggest that there may be alternative mRNA splicing for each of the modulators studied. Differences were noted in the IL-1 alpha specific amplification products in stromal fibroblasts compared to corneal epithelial cells. EGF and EGF receptor mRNA production in human corneal epithelial cells and stromal fibroblasts suggest an autocrine role for EGF in the physiology of each of these cell types. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/1559775/EGF_EGF_receptor_basic_FGF_TGF_beta_1_and_IL_1_alpha_mRNA_in_human_corneal_epithelial_cells_and_stromal_fibroblasts_ L2 - https://iovs.arvojournals.org/article.aspx?volume=33&issue=5&page=1756 DB - PRIME DP - Unbound Medicine ER -