Tags

Type your tag names separated by a space and hit enter

C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage.
Cell Death Differ. 2005 Feb; 12(2):153-61.CD

Abstract

The p53 tumor suppressor promotes apoptosis in response to DNA damage. Here we describe the Caenorhabditis elegans gene ced-13, which encodes a conserved BH3-only protein. We show that ced-13 mRNA accumulates following DNA damage, and that this accumulation is dependent on an intact C. elegans cep-1/p53 gene. We demonstrate that CED-13 protein physically interacts with the antiapoptotic Bcl-2-related protein CED-9. Furthermore, overexpression of ced-13 in somatic cells leads to the death of cells that normally survive, and this death requires the core apoptotic pathway of C. elegans. Recent studies have implicated two BH3-only proteins, Noxa and PUMA, in p53-induced apoptosis in mammals. Our studies suggest that in addition to the BH3-only protein EGL-1, CED-13 might also promote apoptosis in the C. elegans germ line in response to p53 activation. We propose that an evolutionarily conserved pathway exists in which p53 promotes cell death by inducing expression of two BH3-only genes.

Authors+Show Affiliations

Schumacher B
Max-Planck-Institute of Biochemistry, Am Klopferspitz 18A, 82152 Martinsried, Germany.
Schertel C
No affiliation info available
Wittenburg N
No affiliation info available
Tuck S
No affiliation info available
Mitani S
No affiliation info available
Gartner A
No affiliation info available
Conradt B
No affiliation info available
Shaham S
No affiliation info available

MeSH

Amino Acid SequenceAnimalsApoptosisApoptosis Regulatory ProteinsCaenorhabditisCaenorhabditis elegansCaenorhabditis elegans ProteinsDNADNA DamageGene DeletionGene Expression Regulation, DevelopmentalHeat-Shock ProteinsModels, BiologicalMolecular Sequence DataMutationProto-Oncogene ProteinsProto-Oncogene Proteins c-bcl-2Repressor ProteinsSequence Homology, Amino AcidTumor Suppressor Protein p53X-Rays

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15605074

Citation

Schumacher, B, et al. "C. Elegans Ced-13 Can Promote Apoptosis and Is Induced in Response to DNA Damage." Cell Death and Differentiation, vol. 12, no. 2, 2005, pp. 153-61.
Schumacher B, Schertel C, Wittenburg N, et al. C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage. Cell Death Differ. 2005;12(2):153-61.
Schumacher, B., Schertel, C., Wittenburg, N., Tuck, S., Mitani, S., Gartner, A., Conradt, B., & Shaham, S. (2005). C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage. Cell Death and Differentiation, 12(2), 153-61.
Schumacher B, et al. C. Elegans Ced-13 Can Promote Apoptosis and Is Induced in Response to DNA Damage. Cell Death Differ. 2005;12(2):153-61. PubMed PMID: 15605074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage. AU - Schumacher,B, AU - Schertel,C, AU - Wittenburg,N, AU - Tuck,S, AU - Mitani,S, AU - Gartner,A, AU - Conradt,B, AU - Shaham,S, PY - 2004/12/18/pubmed PY - 2005/9/27/medline PY - 2004/12/18/entrez SP - 153 EP - 61 JF - Cell death and differentiation JO - Cell Death Differ VL - 12 IS - 2 N2 - The p53 tumor suppressor promotes apoptosis in response to DNA damage. Here we describe the Caenorhabditis elegans gene ced-13, which encodes a conserved BH3-only protein. We show that ced-13 mRNA accumulates following DNA damage, and that this accumulation is dependent on an intact C. elegans cep-1/p53 gene. We demonstrate that CED-13 protein physically interacts with the antiapoptotic Bcl-2-related protein CED-9. Furthermore, overexpression of ced-13 in somatic cells leads to the death of cells that normally survive, and this death requires the core apoptotic pathway of C. elegans. Recent studies have implicated two BH3-only proteins, Noxa and PUMA, in p53-induced apoptosis in mammals. Our studies suggest that in addition to the BH3-only protein EGL-1, CED-13 might also promote apoptosis in the C. elegans germ line in response to p53 activation. We propose that an evolutionarily conserved pathway exists in which p53 promotes cell death by inducing expression of two BH3-only genes. SN - 1350-9047 UR - https://www.unboundmedicine.com/medline/citation/15605074/C__elegans_ced_13_can_promote_apoptosis_and_is_induced_in_response_to_DNA_damage_ DB - PRIME DP - Unbound Medicine ER -