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Alcohol and polyphenolic grape extract inhibit platelet adhesion in flowing blood.
Eur J Clin Invest. 2004 Dec; 34(12):818-24.EJ

Abstract

BACKGROUND

Moderate and prolonged alcohol consumption has been associated with decreased cardiovascular morbidity and mortality. Inhibition of platelet function in suspension attributes to these effects. Whether alcohol, red wine, or polyphenolic grape extracts (PGE) inhibit platelet adhesion is not known. We investigated platelet adhesion to fibrinogen and collagen in whole blood under standardised flow.

MATERIALS AND METHODS

Before perfusion was started, citrated whole blood from 95 volunteers was preincubated for five min with different alcohol concentrations, unfractioned red wine and PGE. Then, blood was perfused in a single-passage flow chamber over coverslips coated with human fibrinogen or collagen type III at shear rates of 300 s(-1) and 1600 s(-1).

RESULTS

Alcohol inhibited platelet adhesion to human fibrinogen at high shear rate (concentrations > or = 0.15 per thousand) and low shear rate (only at a concentration of 4.8 per thousand), whereas red wine (concentrations > or = 0.15 per thousand) inhibited platelet adhesion to human fibrinogen at both shear rates. In contrast, PGE (concentrations > or = 0.0225 g L(-1)) inhibited platelet adhesion to human fibrinogen only at low shear rate. None of these incubations affected adhesion to collagen.

CONCLUSIONS

Alcohol, red wine and PGE inhibit adhesion to fibrinogen but not to collagen. This inhibition might contribute to the cardioprotective effects of moderate alcohol consumption.

Authors+Show Affiliations

Department of Internal Medicine, Meander Medical Center, Amersfoort, the Netherlands. d.w.delange@azu.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15606724

Citation

de Lange, D W., et al. "Alcohol and Polyphenolic Grape Extract Inhibit Platelet Adhesion in Flowing Blood." European Journal of Clinical Investigation, vol. 34, no. 12, 2004, pp. 818-24.
de Lange DW, Scholman WL, Kraaijenhagen RJ, et al. Alcohol and polyphenolic grape extract inhibit platelet adhesion in flowing blood. Eur J Clin Invest. 2004;34(12):818-24.
de Lange, D. W., Scholman, W. L., Kraaijenhagen, R. J., Akkerman, J. W., & van de Wiel, A. (2004). Alcohol and polyphenolic grape extract inhibit platelet adhesion in flowing blood. European Journal of Clinical Investigation, 34(12), 818-24.
de Lange DW, et al. Alcohol and Polyphenolic Grape Extract Inhibit Platelet Adhesion in Flowing Blood. Eur J Clin Invest. 2004;34(12):818-24. PubMed PMID: 15606724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alcohol and polyphenolic grape extract inhibit platelet adhesion in flowing blood. AU - de Lange,D W, AU - Scholman,W L G, AU - Kraaijenhagen,R J, AU - Akkerman,J W N, AU - van de Wiel,A, PY - 2004/12/21/pubmed PY - 2005/3/15/medline PY - 2004/12/21/entrez SP - 818 EP - 24 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 34 IS - 12 N2 - BACKGROUND: Moderate and prolonged alcohol consumption has been associated with decreased cardiovascular morbidity and mortality. Inhibition of platelet function in suspension attributes to these effects. Whether alcohol, red wine, or polyphenolic grape extracts (PGE) inhibit platelet adhesion is not known. We investigated platelet adhesion to fibrinogen and collagen in whole blood under standardised flow. MATERIALS AND METHODS: Before perfusion was started, citrated whole blood from 95 volunteers was preincubated for five min with different alcohol concentrations, unfractioned red wine and PGE. Then, blood was perfused in a single-passage flow chamber over coverslips coated with human fibrinogen or collagen type III at shear rates of 300 s(-1) and 1600 s(-1). RESULTS: Alcohol inhibited platelet adhesion to human fibrinogen at high shear rate (concentrations > or = 0.15 per thousand) and low shear rate (only at a concentration of 4.8 per thousand), whereas red wine (concentrations > or = 0.15 per thousand) inhibited platelet adhesion to human fibrinogen at both shear rates. In contrast, PGE (concentrations > or = 0.0225 g L(-1)) inhibited platelet adhesion to human fibrinogen only at low shear rate. None of these incubations affected adhesion to collagen. CONCLUSIONS: Alcohol, red wine and PGE inhibit adhesion to fibrinogen but not to collagen. This inhibition might contribute to the cardioprotective effects of moderate alcohol consumption. SN - 0014-2972 UR - https://www.unboundmedicine.com/medline/citation/15606724/Alcohol_and_polyphenolic_grape_extract_inhibit_platelet_adhesion_in_flowing_blood_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2972&date=2004&volume=34&issue=12&spage=818 DB - PRIME DP - Unbound Medicine ER -