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Serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) isolated from dairy cattle in São Paulo State, Brazil.
Vet Microbiol. 2005 Jan 05; 105(1):29-36.VM

Abstract

In order to determine the occurrence, serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) strains, 153 fecal samples of cattle randomly selected from six dairy farms in Sao Paulo State, Brazil, were examined for Shiga toxin (Stx) production by the Vero cell assay. Feces were directly streaked onto MacConkey Sorbitol Agar and incubated at 37 degrees C overnight. Sorbitol-negative colonies (maximum 20) and up to 10 sorbitol-positive colonies from each plate were subcultured onto presumptive diagnostic medium IAL. Sorbitol-negative isolates were screened with O157 antiserum for identification of O157:H7 E. coli. Isolates presenting cytotoxic activity were submitted to colony hybridization assays with specific DNA probes for stx1, stx2, eae, Ehly and astA genes. The isolation rate of STEC ranged from 3.8 to 84.6% depending on the farm analysed. STEC was identified in 25.5% of the animals, and most of them (64.1%) carried a single STEC serotype. A total of 202 STEC isolates were recovered from the animals, and except for the 2 O157:H7 isolates all the others expressed cytotoxic activity. The great majority of the STEC isolates carried both stx1 and stx2 genes (114/202, 56.4%) or stx2 (82/202, 40.6%); and whereas the Ehly sequence occurred in most of them (88%) eae was only observed in O157:H7 and O111:HNM isolates. Serotypes O113:H21, O178:H19 and O79:H14 were the most frequent STEC serotypes identified and widely distributed among animals from different farms, while others such as O77:H18, O88:H25 and O98:H17 occurred only in particular farms. This is the first report on the occurrence of STEC in dairy cattle in Sao Paulo State, and the results point to substantial differences in rate of isolation, serotypes and genetic profile of STEC that has been previously described among beef cattle in our community. Moreover, to our knowledge O79:H14 and O98:H17 represent new STEC serotypes, while O178:H19 has only been recently reported in Spain.

Authors+Show Affiliations

Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351 Cerqueira César, CEP 01246-902, São Paulo, Brazil. ikinue@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15607081

Citation

Irino, K, et al. "Serotypes and Virulence Markers of Shiga Toxin-producing Escherichia Coli (STEC) Isolated From Dairy Cattle in São Paulo State, Brazil." Veterinary Microbiology, vol. 105, no. 1, 2005, pp. 29-36.
Irino K, Kato MA, Vaz TM, et al. Serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) isolated from dairy cattle in São Paulo State, Brazil. Vet Microbiol. 2005;105(1):29-36.
Irino, K., Kato, M. A., Vaz, T. M., Ramos, I. I., Souza, M. A., Cruz, A. S., Gomes, T. A., Vieira, M. A., & Guth, B. E. (2005). Serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) isolated from dairy cattle in São Paulo State, Brazil. Veterinary Microbiology, 105(1), 29-36.
Irino K, et al. Serotypes and Virulence Markers of Shiga Toxin-producing Escherichia Coli (STEC) Isolated From Dairy Cattle in São Paulo State, Brazil. Vet Microbiol. 2005 Jan 5;105(1):29-36. PubMed PMID: 15607081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) isolated from dairy cattle in São Paulo State, Brazil. AU - Irino,K, AU - Kato,M A M F, AU - Vaz,T M I, AU - Ramos,I I, AU - Souza,M A C, AU - Cruz,A S, AU - Gomes,T A T, AU - Vieira,M A M, AU - Guth,B E C, Y1 - 2004/12/10/ PY - 2004/04/16/received PY - 2004/08/17/revised PY - 2004/08/24/accepted PY - 2004/12/21/pubmed PY - 2005/3/16/medline PY - 2004/12/21/entrez SP - 29 EP - 36 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 105 IS - 1 N2 - In order to determine the occurrence, serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) strains, 153 fecal samples of cattle randomly selected from six dairy farms in Sao Paulo State, Brazil, were examined for Shiga toxin (Stx) production by the Vero cell assay. Feces were directly streaked onto MacConkey Sorbitol Agar and incubated at 37 degrees C overnight. Sorbitol-negative colonies (maximum 20) and up to 10 sorbitol-positive colonies from each plate were subcultured onto presumptive diagnostic medium IAL. Sorbitol-negative isolates were screened with O157 antiserum for identification of O157:H7 E. coli. Isolates presenting cytotoxic activity were submitted to colony hybridization assays with specific DNA probes for stx1, stx2, eae, Ehly and astA genes. The isolation rate of STEC ranged from 3.8 to 84.6% depending on the farm analysed. STEC was identified in 25.5% of the animals, and most of them (64.1%) carried a single STEC serotype. A total of 202 STEC isolates were recovered from the animals, and except for the 2 O157:H7 isolates all the others expressed cytotoxic activity. The great majority of the STEC isolates carried both stx1 and stx2 genes (114/202, 56.4%) or stx2 (82/202, 40.6%); and whereas the Ehly sequence occurred in most of them (88%) eae was only observed in O157:H7 and O111:HNM isolates. Serotypes O113:H21, O178:H19 and O79:H14 were the most frequent STEC serotypes identified and widely distributed among animals from different farms, while others such as O77:H18, O88:H25 and O98:H17 occurred only in particular farms. This is the first report on the occurrence of STEC in dairy cattle in Sao Paulo State, and the results point to substantial differences in rate of isolation, serotypes and genetic profile of STEC that has been previously described among beef cattle in our community. Moreover, to our knowledge O79:H14 and O98:H17 represent new STEC serotypes, while O178:H19 has only been recently reported in Spain. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/15607081/Serotypes_and_virulence_markers_of_Shiga_toxin_producing_Escherichia_coli__STEC__isolated_from_dairy_cattle_in_São_Paulo_State_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(04)00316-5 DB - PRIME DP - Unbound Medicine ER -