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Phenotypic and genotypic characteristics of non-O157 Shiga toxin-producing Escherichia coli (STEC) from Swiss cattle.
Vet Microbiol. 2005 Jan 05; 105(1):37-45.VM

Abstract

A total of 42 Shiga toxin-producing (STEC) strains from slaughtered healthy cattle in Switzerland were characterized by phenotypic and genotypic traits. The 42 sorbitol-positive, non-O157 STEC strains belonged to 26 O:H serotypes (including eight new serotypes) with four serotypes (O103:H2, O113:H4, O116:H-, ONT:H-) accounting for 38.1% of strains. Out of 16 serotypes previously found in human STEC (71% of strains), nine serotypes (38% of strains) were serotypes that have been associated with hemolytic-uremic syndrome (HUS). Polymerase chain reaction (PCR) analysis showed that 18 (43%) strains carried the stx1 gene, 20 strains (48%) had the stx2 gene, and four (9%) strains had both stx1 and stx2 genes. Of strains encoding for stx2 variants, 63% were positive for stx2 subtype. Enterohemolysin (ehxA), intimin (eae), STEC autoagglutinating adhesin (saa) were detected in 17%, 21%, and 19% of the strains, respectively. Amongst the seven intimin-positive strains, one possessed intimin type beta1 (O5:H-), one intimin gamma1 (O145:H), one intimin gamma2/theta, (O111:H21), and four intimin epsilon (O103:H2). The strains belonged to 29 serovirotypes (association between serotypes and virulence factors). O103:H2 stx1eae-epsilon ehxA, O116:H- stx2, and ONT:H- stx2c were the most common accounting for 29% of the strains. Only one strain (2.4%) of serovirotype O145:H- stx1stx2eae-gamma1ehxA showed a pattern of highly virulent human strains. This is the first study providing characterization data of bovine non-O157 STEC in Switzerland, and underlining the importance of the determination of virulence factors (including intimin types) in addition to serotypes to assess the potential pathogenicity of these strains for humans.

Authors+Show Affiliations

Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Winterthurerstr. 272, 8057 Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15607082

Citation

Zweifel, C, et al. "Phenotypic and Genotypic Characteristics of non-O157 Shiga Toxin-producing Escherichia Coli (STEC) From Swiss Cattle." Veterinary Microbiology, vol. 105, no. 1, 2005, pp. 37-45.
Zweifel C, Schumacher S, Blanco M, et al. Phenotypic and genotypic characteristics of non-O157 Shiga toxin-producing Escherichia coli (STEC) from Swiss cattle. Vet Microbiol. 2005;105(1):37-45.
Zweifel, C., Schumacher, S., Blanco, M., Blanco, J. E., Tasara, T., Blanco, J., & Stephan, R. (2005). Phenotypic and genotypic characteristics of non-O157 Shiga toxin-producing Escherichia coli (STEC) from Swiss cattle. Veterinary Microbiology, 105(1), 37-45.
Zweifel C, et al. Phenotypic and Genotypic Characteristics of non-O157 Shiga Toxin-producing Escherichia Coli (STEC) From Swiss Cattle. Vet Microbiol. 2005 Jan 5;105(1):37-45. PubMed PMID: 15607082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and genotypic characteristics of non-O157 Shiga toxin-producing Escherichia coli (STEC) from Swiss cattle. AU - Zweifel,C, AU - Schumacher,S, AU - Blanco,M, AU - Blanco,J E, AU - Tasara,T, AU - Blanco,J, AU - Stephan,R, Y1 - 2004/12/08/ PY - 2004/07/24/received PY - 2004/10/04/revised PY - 2004/10/11/accepted PY - 2004/12/21/pubmed PY - 2005/3/16/medline PY - 2004/12/21/entrez SP - 37 EP - 45 JF - Veterinary microbiology JO - Vet Microbiol VL - 105 IS - 1 N2 - A total of 42 Shiga toxin-producing (STEC) strains from slaughtered healthy cattle in Switzerland were characterized by phenotypic and genotypic traits. The 42 sorbitol-positive, non-O157 STEC strains belonged to 26 O:H serotypes (including eight new serotypes) with four serotypes (O103:H2, O113:H4, O116:H-, ONT:H-) accounting for 38.1% of strains. Out of 16 serotypes previously found in human STEC (71% of strains), nine serotypes (38% of strains) were serotypes that have been associated with hemolytic-uremic syndrome (HUS). Polymerase chain reaction (PCR) analysis showed that 18 (43%) strains carried the stx1 gene, 20 strains (48%) had the stx2 gene, and four (9%) strains had both stx1 and stx2 genes. Of strains encoding for stx2 variants, 63% were positive for stx2 subtype. Enterohemolysin (ehxA), intimin (eae), STEC autoagglutinating adhesin (saa) were detected in 17%, 21%, and 19% of the strains, respectively. Amongst the seven intimin-positive strains, one possessed intimin type beta1 (O5:H-), one intimin gamma1 (O145:H), one intimin gamma2/theta, (O111:H21), and four intimin epsilon (O103:H2). The strains belonged to 29 serovirotypes (association between serotypes and virulence factors). O103:H2 stx1eae-epsilon ehxA, O116:H- stx2, and ONT:H- stx2c were the most common accounting for 29% of the strains. Only one strain (2.4%) of serovirotype O145:H- stx1stx2eae-gamma1ehxA showed a pattern of highly virulent human strains. This is the first study providing characterization data of bovine non-O157 STEC in Switzerland, and underlining the importance of the determination of virulence factors (including intimin types) in addition to serotypes to assess the potential pathogenicity of these strains for humans. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/15607082/Phenotypic_and_genotypic_characteristics_of_non_O157_Shiga_toxin_producing_Escherichia_coli__STEC__from_Swiss_cattle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(04)00379-7 DB - PRIME DP - Unbound Medicine ER -