Tags

Type your tag names separated by a space and hit enter

Methylenetetrahydrofolate reductase polymorphism associated with susceptibility to coronary heart disease in Chinese type 2 diabetic patients.
Mol Cell Endocrinol. 2005 Jan 14; 229(1-2):95-101.MC

Abstract

OBJECTIVE

Epidemiological studies have identified hyperhomocyst(e)inemia as an independent risk factor for atherosclerosis. The C677T variant of the methylenetetrahydrofolate reductase (MTHFR) gene, one of the key enzymes catalyzing remethylation of homocysteine, might play a role in the development of coronary heart disease (CHD). In this study, we examined the distribution of the MTHFR genotypes in the Chinese population and the association between the C677T variant and CHD in Chinese type 2 diabetic patients.

METHODS

Two hundred and twenty-eight unrelated patients with type 2 diabetes mellitus (126 with coronary heart disease) and 114 healthy control subjects were recruited. The MTHFR genotype was analyzed by PCR followed by HinfI digestion. Plasma total homocysteine levels were measured using high-performance liquid chromatography (HPLC) with fluorescence detection.

RESULTS

In 114 healthy control subjects, the frequency of the mutant T allele was 38.0%, comparable to that of a Hong Kong (Chinese) population. The genotype distribution did not differ between control subjects and type 2 diabetic patients (chi(2) = 3.67, P > 0.05). Genotypic analysis revealed that type 2 diabetic patients with CHD displayed a greater prevalence of T allele (45.2%) than type 2 diabetic patients without CHD (30.4%) (chi(2) = 8.72, P < 0.005). The odds ratio for CHD in type 2 diabetic patients in presence of T allele was 1.89 (CI 95%, 1.24-2.88). The MTHFR genotype were different between diabetic patients with and without CHD (chi(2) = 11.98, P < 0.005). Moreover, plasma homocysteine levels were markedly higher in individuals with TT genotype than those with CC or CT genotype or CC plus CT genotype.

CONCLUSIONS

The C677T mutation of MTHFR gene is common in the Chinese population. MTHFR C677T gene polymorphism associated with a predisposition to increased plasma homocysteine levels could constitute a useful predictive marker for CHD in Chinese type 2 diabetic patients.

Authors+Show Affiliations

Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China. sjz300@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15607533

Citation

Sun, Jiazhong, et al. "Methylenetetrahydrofolate Reductase Polymorphism Associated With Susceptibility to Coronary Heart Disease in Chinese Type 2 Diabetic Patients." Molecular and Cellular Endocrinology, vol. 229, no. 1-2, 2005, pp. 95-101.
Sun J, Xu Y, Xue J, et al. Methylenetetrahydrofolate reductase polymorphism associated with susceptibility to coronary heart disease in Chinese type 2 diabetic patients. Mol Cell Endocrinol. 2005;229(1-2):95-101.
Sun, J., Xu, Y., Xue, J., Zhu, Y., & Lu, H. (2005). Methylenetetrahydrofolate reductase polymorphism associated with susceptibility to coronary heart disease in Chinese type 2 diabetic patients. Molecular and Cellular Endocrinology, 229(1-2), 95-101.
Sun J, et al. Methylenetetrahydrofolate Reductase Polymorphism Associated With Susceptibility to Coronary Heart Disease in Chinese Type 2 Diabetic Patients. Mol Cell Endocrinol. 2005 Jan 14;229(1-2):95-101. PubMed PMID: 15607533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methylenetetrahydrofolate reductase polymorphism associated with susceptibility to coronary heart disease in Chinese type 2 diabetic patients. AU - Sun,Jiazhong, AU - Xu,Yancheng, AU - Xue,Jinge, AU - Zhu,Yilian, AU - Lu,Hongyun, PY - 2004/04/15/received PY - 2004/09/09/accepted PY - 2004/12/21/pubmed PY - 2005/5/25/medline PY - 2004/12/21/entrez SP - 95 EP - 101 JF - Molecular and cellular endocrinology JO - Mol Cell Endocrinol VL - 229 IS - 1-2 N2 - OBJECTIVE: Epidemiological studies have identified hyperhomocyst(e)inemia as an independent risk factor for atherosclerosis. The C677T variant of the methylenetetrahydrofolate reductase (MTHFR) gene, one of the key enzymes catalyzing remethylation of homocysteine, might play a role in the development of coronary heart disease (CHD). In this study, we examined the distribution of the MTHFR genotypes in the Chinese population and the association between the C677T variant and CHD in Chinese type 2 diabetic patients. METHODS: Two hundred and twenty-eight unrelated patients with type 2 diabetes mellitus (126 with coronary heart disease) and 114 healthy control subjects were recruited. The MTHFR genotype was analyzed by PCR followed by HinfI digestion. Plasma total homocysteine levels were measured using high-performance liquid chromatography (HPLC) with fluorescence detection. RESULTS: In 114 healthy control subjects, the frequency of the mutant T allele was 38.0%, comparable to that of a Hong Kong (Chinese) population. The genotype distribution did not differ between control subjects and type 2 diabetic patients (chi(2) = 3.67, P > 0.05). Genotypic analysis revealed that type 2 diabetic patients with CHD displayed a greater prevalence of T allele (45.2%) than type 2 diabetic patients without CHD (30.4%) (chi(2) = 8.72, P < 0.005). The odds ratio for CHD in type 2 diabetic patients in presence of T allele was 1.89 (CI 95%, 1.24-2.88). The MTHFR genotype were different between diabetic patients with and without CHD (chi(2) = 11.98, P < 0.005). Moreover, plasma homocysteine levels were markedly higher in individuals with TT genotype than those with CC or CT genotype or CC plus CT genotype. CONCLUSIONS: The C677T mutation of MTHFR gene is common in the Chinese population. MTHFR C677T gene polymorphism associated with a predisposition to increased plasma homocysteine levels could constitute a useful predictive marker for CHD in Chinese type 2 diabetic patients. SN - 0303-7207 UR - https://www.unboundmedicine.com/medline/citation/15607533/Methylenetetrahydrofolate_reductase_polymorphism_associated_with_susceptibility_to_coronary_heart_disease_in_Chinese_type_2_diabetic_patients_ DB - PRIME DP - Unbound Medicine ER -