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The influence of dietary vitamin E, fat, and methionine on blood cholesterol profile, homocysteine levels, and oxidizability of low density lipoprotein in the gerbil.
J Nutr Biochem. 2004 Dec; 15(12):730-40.JN

Abstract

A 90-day feeding study with gerbils was conducted to evaluate the influence of dietary vitamin E levels (25 mg/kg diet, 75 mg/kg, 300 mg/kg, and 900 mg/kg), two levels of dietary methionione (casein or casein+L-methionine (1% w/w)) and two sources of lipid (soybean oil [20%] or soybean oil [4%]+coconut oil [16%, 1:4 w/w]) upon serum lipids (total cholesterol, HDL-cholesterol, LDL-cholesterol). In addition, this study examined the effects of diet-induced hyperhomocysteinemia and supplemental dietary vitamin E on the oxidation of low density lipoproteins. Tissue vitamin E (heart, liver, and plasma) demonstrated a dose response (P< or =0.001) following the supplementation with increasing dietary vitamin E (25, 75, 300, and 900 mg/kg). In addition, tissue vitamin E levels were found to be higher (P< or =0.001) in those animals receiving a combination of coconut oil+soybean oil as compared to the group receiving soybean oil solely. Blood cholesterol profiles indicated an increase (P< or =0.001) in total cholesterol and LDL cholesterol by the influence of saturated fat and supplemental methionine. Low-density lipoprotein cholesterol profile demonstrated a reduction (P< or =0.001) at the higher dietary vitamin E levels (300 and 900 mg/kg) as compared to the 25 mg/kg and 75 mg/kg dietary vitamin E. Plasma protein carbonyls were not influenced by dietary vitamin E nor by supplemental methionine intake. In vitro oxidation of LDL showed that vitamin E delayed the lag time of the oxidation phase (P< or =0.001) and reduced total diene production (P< or =0.001). On the contrary, supplemental methionine decreased (P< or =0.001) the delay time of the lag phase, whereas total diene production was increased (P< or =0.001). Plasma lipid hydroperoxides were significantly reduced (P< or =0.05) with supplemental dietary vitamin E, whereas supplemental L-methionine (1%) resulted in a significant (P< or =0.05) increase in lipid plasma hydroperoxide formation. Plasma homocysteine was elevated (P< or =0.001) with supplemental dietary L-methionine (1%) as well as the inclusion of dietary saturated fat. The present data showed that 1) a combination of dietary lipids (saturated and unsaturated fatty acids) as well as vitamin E and methionine supplementation altered blood cholesterol lipoprotein profiles; 2) in vitro oxidation parameters including LDL (lag time and diene production) and plasma hydroperoxide formations were affected by vitamin E and methionine supplementation; and 3) plasma homocysteine concentrations were influenced by supplemental methionine and the inclusion of dietary saturated fat.

Authors+Show Affiliations

Hidiroglou N
Nutrition Research Division, Food Directorate, Health Products and Food Branch, Health Canada, Banting Research Centre, Tunneys Pasture, Ottawa, ON, Canada KIAOL2. Nick_Hidiroglou@hc-sc.gc.ca
Gilani GS
No affiliation info available
Long L
No affiliation info available
Zhao X
No affiliation info available
Madere R
No affiliation info available
Cockell K
No affiliation info available
Belonge B
No affiliation info available
Ratnayake WM
No affiliation info available
Peace R
No affiliation info available

MeSH

AnimalsBlood ProteinsBody WeightCholesterolDietary FatsEatingGerbillinaeHomocysteineLipid PeroxidationLipoproteins, LDLMaleMethionineNutritional StatusVitamin E

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15607646

Citation

Hidiroglou, Nick, et al. "The Influence of Dietary Vitamin E, Fat, and Methionine On Blood Cholesterol Profile, Homocysteine Levels, and Oxidizability of Low Density Lipoprotein in the Gerbil." The Journal of Nutritional Biochemistry, vol. 15, no. 12, 2004, pp. 730-40.
Hidiroglou N, Gilani GS, Long L, et al. The influence of dietary vitamin E, fat, and methionine on blood cholesterol profile, homocysteine levels, and oxidizability of low density lipoprotein in the gerbil. J Nutr Biochem. 2004;15(12):730-40.
Hidiroglou, N., Gilani, G. S., Long, L., Zhao, X., Madere, R., Cockell, K., Belonge, B., Ratnayake, W. M., & Peace, R. (2004). The influence of dietary vitamin E, fat, and methionine on blood cholesterol profile, homocysteine levels, and oxidizability of low density lipoprotein in the gerbil. The Journal of Nutritional Biochemistry, 15(12), 730-40.
Hidiroglou N, et al. The Influence of Dietary Vitamin E, Fat, and Methionine On Blood Cholesterol Profile, Homocysteine Levels, and Oxidizability of Low Density Lipoprotein in the Gerbil. J Nutr Biochem. 2004;15(12):730-40. PubMed PMID: 15607646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of dietary vitamin E, fat, and methionine on blood cholesterol profile, homocysteine levels, and oxidizability of low density lipoprotein in the gerbil. AU - Hidiroglou,Nick, AU - Gilani,G Sarwar, AU - Long,Lori, AU - Zhao,Xin, AU - Madere,Rene, AU - Cockell,Kevin, AU - Belonge,Bart, AU - Ratnayake,W M Nimal, AU - Peace,Robert, PY - 2004/02/04/received PY - 2004/04/15/accepted PY - 2004/12/21/pubmed PY - 2005/4/29/medline PY - 2004/12/21/entrez SP - 730 EP - 40 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 15 IS - 12 N2 - A 90-day feeding study with gerbils was conducted to evaluate the influence of dietary vitamin E levels (25 mg/kg diet, 75 mg/kg, 300 mg/kg, and 900 mg/kg), two levels of dietary methionione (casein or casein+L-methionine (1% w/w)) and two sources of lipid (soybean oil [20%] or soybean oil [4%]+coconut oil [16%, 1:4 w/w]) upon serum lipids (total cholesterol, HDL-cholesterol, LDL-cholesterol). In addition, this study examined the effects of diet-induced hyperhomocysteinemia and supplemental dietary vitamin E on the oxidation of low density lipoproteins. Tissue vitamin E (heart, liver, and plasma) demonstrated a dose response (P< or =0.001) following the supplementation with increasing dietary vitamin E (25, 75, 300, and 900 mg/kg). In addition, tissue vitamin E levels were found to be higher (P< or =0.001) in those animals receiving a combination of coconut oil+soybean oil as compared to the group receiving soybean oil solely. Blood cholesterol profiles indicated an increase (P< or =0.001) in total cholesterol and LDL cholesterol by the influence of saturated fat and supplemental methionine. Low-density lipoprotein cholesterol profile demonstrated a reduction (P< or =0.001) at the higher dietary vitamin E levels (300 and 900 mg/kg) as compared to the 25 mg/kg and 75 mg/kg dietary vitamin E. Plasma protein carbonyls were not influenced by dietary vitamin E nor by supplemental methionine intake. In vitro oxidation of LDL showed that vitamin E delayed the lag time of the oxidation phase (P< or =0.001) and reduced total diene production (P< or =0.001). On the contrary, supplemental methionine decreased (P< or =0.001) the delay time of the lag phase, whereas total diene production was increased (P< or =0.001). Plasma lipid hydroperoxides were significantly reduced (P< or =0.05) with supplemental dietary vitamin E, whereas supplemental L-methionine (1%) resulted in a significant (P< or =0.05) increase in lipid plasma hydroperoxide formation. Plasma homocysteine was elevated (P< or =0.001) with supplemental dietary L-methionine (1%) as well as the inclusion of dietary saturated fat. The present data showed that 1) a combination of dietary lipids (saturated and unsaturated fatty acids) as well as vitamin E and methionine supplementation altered blood cholesterol lipoprotein profiles; 2) in vitro oxidation parameters including LDL (lag time and diene production) and plasma hydroperoxide formations were affected by vitamin E and methionine supplementation; and 3) plasma homocysteine concentrations were influenced by supplemental methionine and the inclusion of dietary saturated fat. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/15607646/The_influence_of_dietary_vitamin_E_fat_and_methionine_on_blood_cholesterol_profile_homocysteine_levels_and_oxidizability_of_low_density_lipoprotein_in_the_gerbil_ DB - PRIME DP - Unbound Medicine ER -