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Identification of differentially expressed genes in yeast Saccharomyces cerevisiae cells with inactivated Mmf1p and Hmf1p, members of proteins family YERO57c/YJGF.
Dev Growth Differ. 2004 Dec; 46(6):545-54.DG

Abstract

We used differential display analysis of mRNA to investigate the differences between gene expression in wild-type (wt) yeast Saccharomyces cerevisiae cells and mutated ones with disrupted activity of genes MMF1 and HMF1, members of the YERO57c/YJGF family. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to determine the differences in the degree of expression of 14 specific transcripts in normal and mutated yeast cells. Obtained data demonstrate that disruption of genes encoding proteins Mmf1p, Hmf1p (or both of them) result in the correlative variation of expression level of the target 12 genes both in the cells with changed phenotype (mmf1 and mmf1 hmf1) and in the cells retaining w.t. shape and growth rate (wt cells, hmf1). Metabolic processes and cellular pathways have been indicated for Mmf1p and Hmf1p based on the different profiles of the expression of 14 genes in mmf1, hmf1 yeast S. cerevisiae cells.

Authors+Show Affiliations

Pozdniakovaite N
Institute of Biotechnology, V. Graiciuno 8, Vilnius, LT-02241, Lithuania.
Popendikyte V
No affiliation info available

MeSH

Gene Expression ProfilingGene Expression Regulation, FungalGene SilencingGenes, FungalMitochondrial ProteinsMutationRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15610144

Citation

Pozdniakovaite, Natalija, and Violeta Popendikyte. "Identification of Differentially Expressed Genes in Yeast Saccharomyces Cerevisiae Cells With Inactivated Mmf1p and Hmf1p, Members of Proteins Family YERO57c/YJGF." Development, Growth & Differentiation, vol. 46, no. 6, 2004, pp. 545-54.
Pozdniakovaite N, Popendikyte V. Identification of differentially expressed genes in yeast Saccharomyces cerevisiae cells with inactivated Mmf1p and Hmf1p, members of proteins family YERO57c/YJGF. Dev Growth Differ. 2004;46(6):545-54.
Pozdniakovaite, N., & Popendikyte, V. (2004). Identification of differentially expressed genes in yeast Saccharomyces cerevisiae cells with inactivated Mmf1p and Hmf1p, members of proteins family YERO57c/YJGF. Development, Growth & Differentiation, 46(6), 545-54.
Pozdniakovaite N, Popendikyte V. Identification of Differentially Expressed Genes in Yeast Saccharomyces Cerevisiae Cells With Inactivated Mmf1p and Hmf1p, Members of Proteins Family YERO57c/YJGF. Dev Growth Differ. 2004;46(6):545-54. PubMed PMID: 15610144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of differentially expressed genes in yeast Saccharomyces cerevisiae cells with inactivated Mmf1p and Hmf1p, members of proteins family YERO57c/YJGF. AU - Pozdniakovaite,Natalija, AU - Popendikyte,Violeta, PY - 2004/12/22/pubmed PY - 2005/6/23/medline PY - 2004/12/22/entrez SP - 545 EP - 54 JF - Development, growth & differentiation JO - Dev Growth Differ VL - 46 IS - 6 N2 - We used differential display analysis of mRNA to investigate the differences between gene expression in wild-type (wt) yeast Saccharomyces cerevisiae cells and mutated ones with disrupted activity of genes MMF1 and HMF1, members of the YERO57c/YJGF family. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to determine the differences in the degree of expression of 14 specific transcripts in normal and mutated yeast cells. Obtained data demonstrate that disruption of genes encoding proteins Mmf1p, Hmf1p (or both of them) result in the correlative variation of expression level of the target 12 genes both in the cells with changed phenotype (mmf1 and mmf1 hmf1) and in the cells retaining w.t. shape and growth rate (wt cells, hmf1). Metabolic processes and cellular pathways have been indicated for Mmf1p and Hmf1p based on the different profiles of the expression of 14 genes in mmf1, hmf1 yeast S. cerevisiae cells. SN - 0012-1592 UR - https://www.unboundmedicine.com/medline/citation/15610144/Identification_of_differentially_expressed_genes_in_yeast_Saccharomyces_cerevisiae_cells_with_inactivated_Mmf1p_and_Hmf1p_members_of_proteins_family_YERO57c/YJGF_ DB - PRIME DP - Unbound Medicine ER -