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[Fluoroquinolone resistance mutations in topoisomerase genes of Salmonella typhimurium isolates].
Wei Sheng Yan Jiu. 2004 Sep; 33(5):591-4.WS

Abstract

OBJECTIVE

Mutations in topoisomerase genes were main cause of the resistence of Salmonella typhimurium to fluoroquinolone.

METHODS

The MICs of three Salmonella typhimurium isolates X2, X7, X11 to ciprofloxacin were above 32 microg/ml, 0.38 microg/ml and 0.023 microg/ml, respectively. The genetic alterations in four topoisomerase genes, gyrA, gyrB, parC, and parE were detected by multiplex PCR amplimer conformation analysis in these three strains.

RESULTS

X2 isolate showed both gyrA mutations (Ser83-->Phe, Asp87-->Asn) and parC mutation (Ser80-->Arg). X7 isolate showed a single gyrA mutation (Ser83-->Phe) and X11 isolate had no changes in all of the four quinolone resistance genes, gyrA, gyrB, parC, and parE. X7 isolate with a single gyrA mutation was less resistant to ciprofloxacin than X2 with double gyrA mutations and an additional parC mutation.

CONCLUSION

GyrA and parC genes play important role of the resistance of Salmonella typhimurium to ciprofloxacin.

Authors+Show Affiliations

Guo Y
Institute of Nutrition and Food Safety, Chinese Center for Disease Prevention and Control, Beijing 100050, China.
Pei X
No affiliation info available
Liu X
No affiliation info available

MeSH

Anti-Bacterial AgentsDNA GyraseDNA Topoisomerase IVDrug Resistance, BacterialFluoroquinolonesMicrobial Sensitivity TestsMutationPolymerase Chain ReactionSalmonella typhimurium

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15612489

Citation

Guo, Yunchang, et al. "[Fluoroquinolone Resistance Mutations in Topoisomerase Genes of Salmonella Typhimurium Isolates]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 33, no. 5, 2004, pp. 591-4.
Guo Y, Pei X, Liu X. [Fluoroquinolone resistance mutations in topoisomerase genes of Salmonella typhimurium isolates]. Wei Sheng Yan Jiu. 2004;33(5):591-4.
Guo, Y., Pei, X., & Liu, X. (2004). [Fluoroquinolone resistance mutations in topoisomerase genes of Salmonella typhimurium isolates]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 33(5), 591-4.
Guo Y, Pei X, Liu X. [Fluoroquinolone Resistance Mutations in Topoisomerase Genes of Salmonella Typhimurium Isolates]. Wei Sheng Yan Jiu. 2004;33(5):591-4. PubMed PMID: 15612489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Fluoroquinolone resistance mutations in topoisomerase genes of Salmonella typhimurium isolates]. AU - Guo,Yunchang, AU - Pei,Xiaoyan, AU - Liu,Xiumei, PY - 2004/12/23/pubmed PY - 2006/10/7/medline PY - 2004/12/23/entrez SP - 591 EP - 4 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 33 IS - 5 N2 - OBJECTIVE: Mutations in topoisomerase genes were main cause of the resistence of Salmonella typhimurium to fluoroquinolone. METHODS: The MICs of three Salmonella typhimurium isolates X2, X7, X11 to ciprofloxacin were above 32 microg/ml, 0.38 microg/ml and 0.023 microg/ml, respectively. The genetic alterations in four topoisomerase genes, gyrA, gyrB, parC, and parE were detected by multiplex PCR amplimer conformation analysis in these three strains. RESULTS: X2 isolate showed both gyrA mutations (Ser83-->Phe, Asp87-->Asn) and parC mutation (Ser80-->Arg). X7 isolate showed a single gyrA mutation (Ser83-->Phe) and X11 isolate had no changes in all of the four quinolone resistance genes, gyrA, gyrB, parC, and parE. X7 isolate with a single gyrA mutation was less resistant to ciprofloxacin than X2 with double gyrA mutations and an additional parC mutation. CONCLUSION: GyrA and parC genes play important role of the resistance of Salmonella typhimurium to ciprofloxacin. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/15612489/[Fluoroquinolone_resistance_mutations_in_topoisomerase_genes_of_Salmonella_typhimurium_isolates]_ L2 - https://medlineplus.gov/antibioticresistance.html DB - PRIME DP - Unbound Medicine ER -