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Impact of altered substrate utilization on cardiac function in isolated hearts from Zucker diabetic fatty rats.
Am J Physiol Heart Circ Physiol 2005; 288(5):H2102-10AJ

Abstract

The goal of this study was to determine whether changes in cardiac metabolism in Type 2 diabetes are associated with contractile dysfunction or impaired response to ischemia. Hearts from Zucker diabetic fatty (ZDF) and lean control rats were isolated and perfused with glucose, lactate, pyruvate, and palmitate. The rates of glucose, lactate, pyruvate, and palmitate oxidation rates and glycolysis were determined during baseline perfusion and low-flow ischemia (LFI; 0.3 ml/min for 30 min) and after LFI and reperfusion. Under all conditions, ATP synthesis from palmitate was increased and synthesis from lactate was decreased in the ZDF group, whereas the contribution from glucose was unchanged. During baseline perfusion, the rate of glycolysis was lower in the ZDF group; however, during LFI and reperfusion, there were no differences between groups. Despite these metabolic shifts, there were no differences in oxygen consumption or ATP production rates between the groups under any perfusion conditions. Cardiac function was slightly depressed before LFI in the ZDF group, but during reperfusion, function was improved relative to the control group despite the increased dependence on fatty acids for energy production. These data suggest that in this model of diabetes, the shift from carbohydrates to fatty acids for oxidative energy production did not increase myocardial oxygen consumption and was not associated with impaired response to ischemia and reperfusion.

Authors+Show Affiliations

Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294-0005, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15615844

Citation

Wang, Peipei, et al. "Impact of Altered Substrate Utilization On Cardiac Function in Isolated Hearts From Zucker Diabetic Fatty Rats." American Journal of Physiology. Heart and Circulatory Physiology, vol. 288, no. 5, 2005, pp. H2102-10.
Wang P, Lloyd SG, Zeng H, et al. Impact of altered substrate utilization on cardiac function in isolated hearts from Zucker diabetic fatty rats. Am J Physiol Heart Circ Physiol. 2005;288(5):H2102-10.
Wang, P., Lloyd, S. G., Zeng, H., Bonen, A., & Chatham, J. C. (2005). Impact of altered substrate utilization on cardiac function in isolated hearts from Zucker diabetic fatty rats. American Journal of Physiology. Heart and Circulatory Physiology, 288(5), pp. H2102-10.
Wang P, et al. Impact of Altered Substrate Utilization On Cardiac Function in Isolated Hearts From Zucker Diabetic Fatty Rats. Am J Physiol Heart Circ Physiol. 2005;288(5):H2102-10. PubMed PMID: 15615844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of altered substrate utilization on cardiac function in isolated hearts from Zucker diabetic fatty rats. AU - Wang,Peipei, AU - Lloyd,Steven G, AU - Zeng,Huadong, AU - Bonen,Arend, AU - Chatham,John C, Y1 - 2004/12/22/ PY - 2004/12/24/pubmed PY - 2005/6/1/medline PY - 2004/12/24/entrez SP - H2102 EP - 10 JF - American journal of physiology. Heart and circulatory physiology JO - Am. J. Physiol. Heart Circ. Physiol. VL - 288 IS - 5 N2 - The goal of this study was to determine whether changes in cardiac metabolism in Type 2 diabetes are associated with contractile dysfunction or impaired response to ischemia. Hearts from Zucker diabetic fatty (ZDF) and lean control rats were isolated and perfused with glucose, lactate, pyruvate, and palmitate. The rates of glucose, lactate, pyruvate, and palmitate oxidation rates and glycolysis were determined during baseline perfusion and low-flow ischemia (LFI; 0.3 ml/min for 30 min) and after LFI and reperfusion. Under all conditions, ATP synthesis from palmitate was increased and synthesis from lactate was decreased in the ZDF group, whereas the contribution from glucose was unchanged. During baseline perfusion, the rate of glycolysis was lower in the ZDF group; however, during LFI and reperfusion, there were no differences between groups. Despite these metabolic shifts, there were no differences in oxygen consumption or ATP production rates between the groups under any perfusion conditions. Cardiac function was slightly depressed before LFI in the ZDF group, but during reperfusion, function was improved relative to the control group despite the increased dependence on fatty acids for energy production. These data suggest that in this model of diabetes, the shift from carbohydrates to fatty acids for oxidative energy production did not increase myocardial oxygen consumption and was not associated with impaired response to ischemia and reperfusion. SN - 0363-6135 UR - https://www.unboundmedicine.com/medline/citation/15615844/Impact_of_altered_substrate_utilization_on_cardiac_function_in_isolated_hearts_from_Zucker_diabetic_fatty_rats_ L2 - http://www.physiology.org/doi/full/10.1152/ajpheart.00935.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -