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Hyper-expression of osteocalcin mRNA in odontoblasts of Hyp mice.
J Dent Res 2005; 84(1):84-8JD

Abstract

The Hyp mouse is a murine homologue of human X-linked hypophosphatemia that displays hypo-mineralization in bone and dentin. In this study, we tested the hypothesis that the defect in Hyp mice leads to alterations in the expression of dentin matrix proteins that may be associated with the hypo-mineralization changes in the tissues. Quantitative RT-PCR analyses showed that expression of the osteocalcin gene in Hyp mice tooth germ samples was significantly higher than in wild-type mice, whereas the gene expressions of osteonectin, osteopontin, dentin matrix protein 1, and type I collagen in both types of mice were similar. Further, cultured Hyp mice tooth germ samples exhibited a higher expression of the osteocalcin gene than did those from wild-type mice, which was in accord with the results of our in vivo analysis. These findings suggest that osteocalcin mRNA is highly expressed in Hyp mice odontoblasts and may be associated with dentin hypo-mineralization.

Authors+Show Affiliations

Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15615882

Citation

Onishi, T, et al. "Hyper-expression of Osteocalcin mRNA in Odontoblasts of Hyp Mice." Journal of Dental Research, vol. 84, no. 1, 2005, pp. 84-8.
Onishi T, Ogawa T, Hayashibara T, et al. Hyper-expression of osteocalcin mRNA in odontoblasts of Hyp mice. J Dent Res. 2005;84(1):84-8.
Onishi, T., Ogawa, T., Hayashibara, T., Hoshino, T., Okawa, R., & Ooshima, T. (2005). Hyper-expression of osteocalcin mRNA in odontoblasts of Hyp mice. Journal of Dental Research, 84(1), pp. 84-8.
Onishi T, et al. Hyper-expression of Osteocalcin mRNA in Odontoblasts of Hyp Mice. J Dent Res. 2005;84(1):84-8. PubMed PMID: 15615882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hyper-expression of osteocalcin mRNA in odontoblasts of Hyp mice. AU - Onishi,T, AU - Ogawa,T, AU - Hayashibara,T, AU - Hoshino,T, AU - Okawa,R, AU - Ooshima,T, PY - 2004/12/24/pubmed PY - 2005/2/8/medline PY - 2004/12/24/entrez SP - 84 EP - 8 JF - Journal of dental research JO - J. Dent. Res. VL - 84 IS - 1 N2 - The Hyp mouse is a murine homologue of human X-linked hypophosphatemia that displays hypo-mineralization in bone and dentin. In this study, we tested the hypothesis that the defect in Hyp mice leads to alterations in the expression of dentin matrix proteins that may be associated with the hypo-mineralization changes in the tissues. Quantitative RT-PCR analyses showed that expression of the osteocalcin gene in Hyp mice tooth germ samples was significantly higher than in wild-type mice, whereas the gene expressions of osteonectin, osteopontin, dentin matrix protein 1, and type I collagen in both types of mice were similar. Further, cultured Hyp mice tooth germ samples exhibited a higher expression of the osteocalcin gene than did those from wild-type mice, which was in accord with the results of our in vivo analysis. These findings suggest that osteocalcin mRNA is highly expressed in Hyp mice odontoblasts and may be associated with dentin hypo-mineralization. SN - 0022-0345 UR - https://www.unboundmedicine.com/medline/citation/15615882/Hyper-expression_of_osteocalcin_mRNA_in_odontoblasts_of_Hyp_mice L2 - http://journals.sagepub.com/doi/full/10.1177/154405910508400115?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -