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Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.
Plant Physiol. 2005 Jan; 137(1):43-56.PP

Abstract

To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of wheat (Triticum aestivum), and the introduced fragment effectively included the gene for ISA1 for wheat (TaISA1) that was encoded on the D genome. In TaISA1-expressing rice endosperm, phytoglycogen synthesis was substantially replaced by starch synthesis, leaving only residual levels of phytoglycogen. The levels of residual phytoglycogen present were inversely proportional to the expression level of the TaISA1 protein, although the level of pullulanase that had been reduced in EM914 was restored to the same level as that in the wild type. Small but significant differences were found in the amylopectin chain-length distribution, gelatinization temperatures, and A-type x-ray diffraction patterns of the starches from lines expressing TaISA1 when compared with wild-type rice starch, although in the first two parameters, the effect was proportional to the expression level of TaISA. The impact of expression levels of ISA1 on starch structure and properties provides support for the view that ISA1 is directly involved in the synthesis of amylopectin.

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Authors+Show Affiliations

Kubo A
Akita Prefectural University, Akita 010-0195, Japan.
Rahman S
No affiliation info available
Utsumi Y
No affiliation info available
Li Z
No affiliation info available
Mukai Y
No affiliation info available
Yamamoto M
No affiliation info available
Ugaki M
No affiliation info available
Harada K
No affiliation info available
Satoh H
No affiliation info available
Konik-Rose C
No affiliation info available
Morell M
No affiliation info available
Nakamura Y
No affiliation info available

MeSH

AmylopectinGene Expression Regulation, PlantGenetic Complementation TestIsoamylaseIsoenzymesMolecular Sequence DataOryzaPhenotypePlants, Genetically ModifiedSeedsStarchTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15618430

Citation

Kubo, Akiko, et al. "Complementation of Sugary-1 Phenotype in Rice Endosperm With the Wheat Isoamylase1 Gene Supports a Direct Role for Isoamylase1 in Amylopectin Biosynthesis." Plant Physiology, vol. 137, no. 1, 2005, pp. 43-56.
Kubo A, Rahman S, Utsumi Y, et al. Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis. Plant Physiol. 2005;137(1):43-56.
Kubo, A., Rahman, S., Utsumi, Y., Li, Z., Mukai, Y., Yamamoto, M., Ugaki, M., Harada, K., Satoh, H., Konik-Rose, C., Morell, M., & Nakamura, Y. (2005). Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis. Plant Physiology, 137(1), 43-56.
Kubo A, et al. Complementation of Sugary-1 Phenotype in Rice Endosperm With the Wheat Isoamylase1 Gene Supports a Direct Role for Isoamylase1 in Amylopectin Biosynthesis. Plant Physiol. 2005;137(1):43-56. PubMed PMID: 15618430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis. AU - Kubo,Akiko, AU - Rahman,Sadequr, AU - Utsumi,Yoshinori, AU - Li,Zhongyi, AU - Mukai,Yasuhiko, AU - Yamamoto,Maki, AU - Ugaki,Masashi, AU - Harada,Kyuya, AU - Satoh,Hikaru, AU - Konik-Rose,Christine, AU - Morell,Matthew, AU - Nakamura,Yasunori, Y1 - 2004/12/23/ PY - 2004/12/25/pubmed PY - 2005/5/25/medline PY - 2004/12/25/entrez SP - 43 EP - 56 JF - Plant physiology JO - Plant Physiol VL - 137 IS - 1 N2 - To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of wheat (Triticum aestivum), and the introduced fragment effectively included the gene for ISA1 for wheat (TaISA1) that was encoded on the D genome. In TaISA1-expressing rice endosperm, phytoglycogen synthesis was substantially replaced by starch synthesis, leaving only residual levels of phytoglycogen. The levels of residual phytoglycogen present were inversely proportional to the expression level of the TaISA1 protein, although the level of pullulanase that had been reduced in EM914 was restored to the same level as that in the wild type. Small but significant differences were found in the amylopectin chain-length distribution, gelatinization temperatures, and A-type x-ray diffraction patterns of the starches from lines expressing TaISA1 when compared with wild-type rice starch, although in the first two parameters, the effect was proportional to the expression level of TaISA. The impact of expression levels of ISA1 on starch structure and properties provides support for the view that ISA1 is directly involved in the synthesis of amylopectin. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/15618430/Complementation_of_sugary_1_phenotype_in_rice_endosperm_with_the_wheat_isoamylase1_gene_supports_a_direct_role_for_isoamylase1_in_amylopectin_biosynthesis_ DB - PRIME DP - Unbound Medicine ER -