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Silver stains distinguish tau-positive structures in corticobasal degeneration/progressive supranuclear palsy and in Alzheimer's disease--comparison between Gallyas and Campbell-Switzer methods.
Acta Neuropathol. 2005 Mar; 109(3):299-305.AN

Abstract

Possible differences in silver-staining profiles and their relation to tau-like immunoreactivity were investigated on cortical sections from corticobasal degeneration (CBD), progressive supranuclear palsy (PSP), Down's syndrome (DS) and Alzheimer's disease (AD). Pairs of mirror sections were double-fluorolabeled with an anti-PHF tau (AT8) antibody and thiazin red (TR), a fluorochrome that labels fibrillary structures such as neurofibrillary tangles (NFTs). Subsequently, one of the pair was stained with Gallyas method (GAL), and the other with Campbell-Switzer method (CS). Identification of the same structure on the corresponding microscopic fields enabled a comparison of four different profiles of each structure: AT8 immunoreactivity, and affinity to TR, GAL and CS. NFTs of DS/AD, containing three- and four-repeat tau, were positive for TR, GAL and CS. AT8-immunoreactive structures of CBD/PSP, containing mainly four-repeat tau, were positive for GAL, but negative for CS and TR. This discrepancy is explainable if the argyrophilia with GAL is related to deposits containing four-repeat tau, while that with CS is linked to those containing three-repeat tau. The lack of CS labeling may also be related to poor TR staining, possibly representing scarcity of fibrillary structure in CBD/PSP. The absence of CS staining is characteristic of tau-positive structures of CBD/PSP, which is readily distinguishable from NFTs of DS/AD, hence is of potential pathological and diagnostic relevance.

Authors+Show Affiliations

Department of Neuropathology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashi-dai, Fuchu, 183-8526, Tokyo, Japan. uchihara@tmin.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15619127

Citation

Uchihara, Toshiki, et al. "Silver Stains Distinguish Tau-positive Structures in Corticobasal Degeneration/progressive Supranuclear Palsy and in Alzheimer's Disease--comparison Between Gallyas and Campbell-Switzer Methods." Acta Neuropathologica, vol. 109, no. 3, 2005, pp. 299-305.
Uchihara T, Shibuya K, Nakamura A, et al. Silver stains distinguish tau-positive structures in corticobasal degeneration/progressive supranuclear palsy and in Alzheimer's disease--comparison between Gallyas and Campbell-Switzer methods. Acta Neuropathol. 2005;109(3):299-305.
Uchihara, T., Shibuya, K., Nakamura, A., & Yagishita, S. (2005). Silver stains distinguish tau-positive structures in corticobasal degeneration/progressive supranuclear palsy and in Alzheimer's disease--comparison between Gallyas and Campbell-Switzer methods. Acta Neuropathologica, 109(3), 299-305.
Uchihara T, et al. Silver Stains Distinguish Tau-positive Structures in Corticobasal Degeneration/progressive Supranuclear Palsy and in Alzheimer's Disease--comparison Between Gallyas and Campbell-Switzer Methods. Acta Neuropathol. 2005;109(3):299-305. PubMed PMID: 15619127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver stains distinguish tau-positive structures in corticobasal degeneration/progressive supranuclear palsy and in Alzheimer's disease--comparison between Gallyas and Campbell-Switzer methods. AU - Uchihara,Toshiki, AU - Shibuya,Katsuhiko, AU - Nakamura,Ayako, AU - Yagishita,Saburo, Y1 - 2004/12/24/ PY - 2004/09/03/received PY - 2004/10/18/accepted PY - 2004/10/15/revised PY - 2004/12/25/pubmed PY - 2005/9/2/medline PY - 2004/12/25/entrez SP - 299 EP - 305 JF - Acta neuropathologica JO - Acta Neuropathol VL - 109 IS - 3 N2 - Possible differences in silver-staining profiles and their relation to tau-like immunoreactivity were investigated on cortical sections from corticobasal degeneration (CBD), progressive supranuclear palsy (PSP), Down's syndrome (DS) and Alzheimer's disease (AD). Pairs of mirror sections were double-fluorolabeled with an anti-PHF tau (AT8) antibody and thiazin red (TR), a fluorochrome that labels fibrillary structures such as neurofibrillary tangles (NFTs). Subsequently, one of the pair was stained with Gallyas method (GAL), and the other with Campbell-Switzer method (CS). Identification of the same structure on the corresponding microscopic fields enabled a comparison of four different profiles of each structure: AT8 immunoreactivity, and affinity to TR, GAL and CS. NFTs of DS/AD, containing three- and four-repeat tau, were positive for TR, GAL and CS. AT8-immunoreactive structures of CBD/PSP, containing mainly four-repeat tau, were positive for GAL, but negative for CS and TR. This discrepancy is explainable if the argyrophilia with GAL is related to deposits containing four-repeat tau, while that with CS is linked to those containing three-repeat tau. The lack of CS labeling may also be related to poor TR staining, possibly representing scarcity of fibrillary structure in CBD/PSP. The absence of CS staining is characteristic of tau-positive structures of CBD/PSP, which is readily distinguishable from NFTs of DS/AD, hence is of potential pathological and diagnostic relevance. SN - 0001-6322 UR - https://www.unboundmedicine.com/medline/citation/15619127/Silver_stains_distinguish_tau_positive_structures_in_corticobasal_degeneration/progressive_supranuclear_palsy_and_in_Alzheimer's_disease__comparison_between_Gallyas_and_Campbell_Switzer_methods_ L2 - https://dx.doi.org/10.1007/s00401-004-0947-7 DB - PRIME DP - Unbound Medicine ER -