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Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling.
Pest Manag Sci. 2005 Feb; 61(2):197-203.PM

Abstract

We describe a detailed study on the possibility of analyzing aminated pesticides and metabolites using pre-column derivatization with fluorescein isothiocyanate (FITC) and their subsequent separation and detection by cyclodextrin capillary electrophoresis-laser-induced fluorescence (CE-LIF) detection. Different variables affecting the derivatization reaction (pH, FITC concentration, reaction time and temperature) and those related with the separation itself (buffer concentration, addition of various organic modifiers, pH, applied voltage and injection time) were studied. The limit of detection obtained was between 0.45 and 3.48 microg litre(-1) showing a relative standard deviation between 0.26 and 2.08% at a concentration level of 50 microg litre(-1).

Authors+Show Affiliations

Navarrete-Casas M
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, E-18071 Granada, Spain.
Segura-Carretero A
No affiliation info available
Cruces-Blanco C
No affiliation info available
Fernández-Gutiérrez A
No affiliation info available

MeSH

AminesCyclodextrinsElectrophoresis, CapillaryFluorescein-5-isothiocyanateFluorescent DyesMolecular StructurePesticidesReproducibility of ResultsSensitivity and SpecificitySpectrometry, FluorescenceWater Pollutants, Chemical

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15619713

Citation

Navarrete-Casas, Miguel, et al. "Potential Determination of Aminated Pesticides and Metabolites By Cyclodextrin Capillary Electrophoresis-laser-induced Fluorescence Using FITC as Labelling." Pest Management Science, vol. 61, no. 2, 2005, pp. 197-203.
Navarrete-Casas M, Segura-Carretero A, Cruces-Blanco C, et al. Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling. Pest Manag Sci. 2005;61(2):197-203.
Navarrete-Casas, M., Segura-Carretero, A., Cruces-Blanco, C., & Fernández-Gutiérrez, A. (2005). Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling. Pest Management Science, 61(2), 197-203.
Navarrete-Casas M, et al. Potential Determination of Aminated Pesticides and Metabolites By Cyclodextrin Capillary Electrophoresis-laser-induced Fluorescence Using FITC as Labelling. Pest Manag Sci. 2005;61(2):197-203. PubMed PMID: 15619713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis-laser-induced fluorescence using FITC as labelling. AU - Navarrete-Casas,Miguel, AU - Segura-Carretero,Antonio, AU - Cruces-Blanco,Carmen, AU - Fernández-Gutiérrez,Alberto, PY - 2004/12/28/pubmed PY - 2005/2/26/medline PY - 2004/12/28/entrez SP - 197 EP - 203 JF - Pest management science JO - Pest Manag Sci VL - 61 IS - 2 N2 - We describe a detailed study on the possibility of analyzing aminated pesticides and metabolites using pre-column derivatization with fluorescein isothiocyanate (FITC) and their subsequent separation and detection by cyclodextrin capillary electrophoresis-laser-induced fluorescence (CE-LIF) detection. Different variables affecting the derivatization reaction (pH, FITC concentration, reaction time and temperature) and those related with the separation itself (buffer concentration, addition of various organic modifiers, pH, applied voltage and injection time) were studied. The limit of detection obtained was between 0.45 and 3.48 microg litre(-1) showing a relative standard deviation between 0.26 and 2.08% at a concentration level of 50 microg litre(-1). SN - 1526-498X UR - https://www.unboundmedicine.com/medline/citation/15619713/Potential_determination_of_aminated_pesticides_and_metabolites_by_cyclodextrin_capillary_electrophoresis_laser_induced_fluorescence_using_FITC_as_labelling_ DB - PRIME DP - Unbound Medicine ER -
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