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Constitutive intracellular expression of human leukocyte antigen (HLA)-DO and HLA-DR but not HLA-DM in trophoblast cells.
Hum Immunol. 2005 Jan; 66(1):43-55.HI

Abstract

The nonclassic human leukocyte antigen (HLA)-DM molecules have been proved to positively regulate antigen presentation in classic antigen-presenting cells, whereas in B lymphocytes HLA-DO have been identified as negative regulators of the process. The present report examines whether the negative expression of classic class II molecules in trophoblasts implies negative regulation by HLA-DO. It was revealed by immunofluorescence, confocal microscopy, and subcellular fractionation techniques that human trophoblasts, although not expressing any surface HLA-DR antigens, constitutively express intracellular HLA-DR, HLA-DO, and CD74, but not HLA-DM. Administration of interferon-gamma to the cell culture increased HLA-DR and CD74, induced HLA-DM, but did not alter the expression of HLA-DO and induced HLA-DR release from the cells. These results were confirmed by reverse transcriptase-polymerase chain reaction analysis except that HLA-DM mRNA was detected in control cells, indicating a posttranscriptional regulation. Under the same experimental conditions, human monocytes/macrophages were not expressing intracellular HLA-DO while exhibiting significant levels of HLA-DR, HLA-DM, and CD74. The results presented here reveal for the first time expression of HLA-DO in trophoblasts, which can be of great importance in maintaining the class II-negative state in these cells and consequently protecting the fetus from maternal immune attack.

Authors+Show Affiliations

Department of Biology, Laboratory of Immunology, University of Crete, Heraklion, Crete, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15620461

Citation

Ranella, Anthi, et al. "Constitutive Intracellular Expression of Human Leukocyte Antigen (HLA)-DO and HLA-DR but Not HLA-DM in Trophoblast Cells." Human Immunology, vol. 66, no. 1, 2005, pp. 43-55.
Ranella A, Vassiliadis S, Mastora C, et al. Constitutive intracellular expression of human leukocyte antigen (HLA)-DO and HLA-DR but not HLA-DM in trophoblast cells. Hum Immunol. 2005;66(1):43-55.
Ranella, A., Vassiliadis, S., Mastora, C., Valentina, M., Dionyssopoulou, E., & Athanassakis, I. (2005). Constitutive intracellular expression of human leukocyte antigen (HLA)-DO and HLA-DR but not HLA-DM in trophoblast cells. Human Immunology, 66(1), 43-55.
Ranella A, et al. Constitutive Intracellular Expression of Human Leukocyte Antigen (HLA)-DO and HLA-DR but Not HLA-DM in Trophoblast Cells. Hum Immunol. 2005;66(1):43-55. PubMed PMID: 15620461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Constitutive intracellular expression of human leukocyte antigen (HLA)-DO and HLA-DR but not HLA-DM in trophoblast cells. AU - Ranella,Anthi, AU - Vassiliadis,Simon, AU - Mastora,Chrisa, AU - Valentina,Michailidou, AU - Dionyssopoulou,Eva, AU - Athanassakis,Irene, PY - 2004/07/15/received PY - 2004/10/05/revised PY - 2004/10/07/accepted PY - 2004/12/29/pubmed PY - 2005/10/12/medline PY - 2004/12/29/entrez SP - 43 EP - 55 JF - Human immunology JO - Hum Immunol VL - 66 IS - 1 N2 - The nonclassic human leukocyte antigen (HLA)-DM molecules have been proved to positively regulate antigen presentation in classic antigen-presenting cells, whereas in B lymphocytes HLA-DO have been identified as negative regulators of the process. The present report examines whether the negative expression of classic class II molecules in trophoblasts implies negative regulation by HLA-DO. It was revealed by immunofluorescence, confocal microscopy, and subcellular fractionation techniques that human trophoblasts, although not expressing any surface HLA-DR antigens, constitutively express intracellular HLA-DR, HLA-DO, and CD74, but not HLA-DM. Administration of interferon-gamma to the cell culture increased HLA-DR and CD74, induced HLA-DM, but did not alter the expression of HLA-DO and induced HLA-DR release from the cells. These results were confirmed by reverse transcriptase-polymerase chain reaction analysis except that HLA-DM mRNA was detected in control cells, indicating a posttranscriptional regulation. Under the same experimental conditions, human monocytes/macrophages were not expressing intracellular HLA-DO while exhibiting significant levels of HLA-DR, HLA-DM, and CD74. The results presented here reveal for the first time expression of HLA-DO in trophoblasts, which can be of great importance in maintaining the class II-negative state in these cells and consequently protecting the fetus from maternal immune attack. SN - 0198-8859 UR - https://www.unboundmedicine.com/medline/citation/15620461/Constitutive_intracellular_expression_of_human_leukocyte_antigen__HLA__DO_and_HLA_DR_but_not_HLA_DM_in_trophoblast_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0198-8859(04)00644-5 DB - PRIME DP - Unbound Medicine ER -