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Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of L-DOPA prodrugs by mean of HPLC-EC.
J Pharm Biomed Anal. 2005 Jan 04; 36(5):1079-84.JP

Abstract

A high performance liquid chromatography (HPLC) method was developed to detected simultaneously L-dihydroxyphenylalanine (L-DOPA), dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum dilaysates following oral administration of L-DOPA or its prodrugs. The chromatographic system uses a reversed-phase C18 column with electrochemical detection at +0.30 V. Mobile phase consisted of 0.05 M citric acid, sodium EDTA 50 microM, sodium octylsulphonate 0.4 nM at pH of 2.9 and 8% methanol (v/v) at a flow rate of 1 ml/min. The calibration curves were linear over the concentration range of 10nm to 100 microM and the lower limits of detections were 125 fmol for L-DOPA, 50 fmol for DOPAC, 250 fmol for DA and 150 fmol for HVA at signal noise to ratio of 3. The repeatability (or intra-day precision), expressed by the relative standard deviation, were better than 4%. The construction of microdialysis probes has been described. The in vitro relative recoveries of each microdialysis probe were evaluated and the results show that they are similar and reproducible for all the analytes with CVs from 1 to 4%. The HPLC-EC method was applied to detect the extracellular levels of L-DOPA, DA, DOPAC and HVA in the striatum dialysates of freely moving rats after oral administration of six new potential L-DOPA prodrugs.

Authors+Show Affiliations

Dipartimento di Scienze Farmaceutiche, Università degli studi di Modena e Reggio Emilia, Via Campi 183, 41100 Modena, Italy. pippi@unimo.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15620535

Citation

Cannazza, G, et al. "Detection of Levodopa, Dopamine and Its Metabolites in Rat Striatum Dialysates Following Peripheral Administration of L-DOPA Prodrugs By Mean of HPLC-EC." Journal of Pharmaceutical and Biomedical Analysis, vol. 36, no. 5, 2005, pp. 1079-84.
Cannazza G, Di Stefano A, Mosciatti B, et al. Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of L-DOPA prodrugs by mean of HPLC-EC. J Pharm Biomed Anal. 2005;36(5):1079-84.
Cannazza, G., Di Stefano, A., Mosciatti, B., Braghiroli, D., Baraldi, M., Pinnen, F., Sozio, P., Benatti, C., & Parenti, C. (2005). Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of L-DOPA prodrugs by mean of HPLC-EC. Journal of Pharmaceutical and Biomedical Analysis, 36(5), 1079-84.
Cannazza G, et al. Detection of Levodopa, Dopamine and Its Metabolites in Rat Striatum Dialysates Following Peripheral Administration of L-DOPA Prodrugs By Mean of HPLC-EC. J Pharm Biomed Anal. 2005 Jan 4;36(5):1079-84. PubMed PMID: 15620535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of L-DOPA prodrugs by mean of HPLC-EC. AU - Cannazza,G, AU - Di Stefano,A, AU - Mosciatti,B, AU - Braghiroli,D, AU - Baraldi,M, AU - Pinnen,F, AU - Sozio,P, AU - Benatti,C, AU - Parenti,C, PY - 2004/05/17/received PY - 2004/09/17/revised PY - 2004/09/17/accepted PY - 2004/12/29/pubmed PY - 2005/6/4/medline PY - 2004/12/29/entrez SP - 1079 EP - 84 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 36 IS - 5 N2 - A high performance liquid chromatography (HPLC) method was developed to detected simultaneously L-dihydroxyphenylalanine (L-DOPA), dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum dilaysates following oral administration of L-DOPA or its prodrugs. The chromatographic system uses a reversed-phase C18 column with electrochemical detection at +0.30 V. Mobile phase consisted of 0.05 M citric acid, sodium EDTA 50 microM, sodium octylsulphonate 0.4 nM at pH of 2.9 and 8% methanol (v/v) at a flow rate of 1 ml/min. The calibration curves were linear over the concentration range of 10nm to 100 microM and the lower limits of detections were 125 fmol for L-DOPA, 50 fmol for DOPAC, 250 fmol for DA and 150 fmol for HVA at signal noise to ratio of 3. The repeatability (or intra-day precision), expressed by the relative standard deviation, were better than 4%. The construction of microdialysis probes has been described. The in vitro relative recoveries of each microdialysis probe were evaluated and the results show that they are similar and reproducible for all the analytes with CVs from 1 to 4%. The HPLC-EC method was applied to detect the extracellular levels of L-DOPA, DA, DOPAC and HVA in the striatum dialysates of freely moving rats after oral administration of six new potential L-DOPA prodrugs. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15620535/Detection_of_levodopa_dopamine_and_its_metabolites_in_rat_striatum_dialysates_following_peripheral_administration_of_L_DOPA_prodrugs_by_mean_of_HPLC_EC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(04)00443-1 DB - PRIME DP - Unbound Medicine ER -