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Restoration of expression of transforming growth factor-beta type II receptor in murine renal cell carcinoma (renca) cells by 5-Aza-2'-deoxycytidine.
Life Sci. 2005 Jan 21; 76(10):1159-66.LS

Abstract

The murine renal cell carcinoma (Renca) cells are insensitive to TGF-beta due to a lack of TGF-beta type II receptor (TbetaR-II). The objective of the present study is to determine the mechanism of this loss of sensitivity to TGF-beta in Renca cells. Renca cells were cultured and treated with 5-Aza-2'-Deoxycytidine (5-Aza), a specific inhibitor of methylation. Expression of TGF-beta type I receptor (TbetaRI) and TbetaRII was determined by RT-PCR and Western blot analysis before and after the treatment of Renca cells with 5-Aza. The expression of phosphorylated Smad2 (P-Smad2) was determined by Western blot analysis. TGF-beta levels in the conditioned medium were measured by ELISA. Renca cells did not express TbetaR-II prior to 5-Aza treatment. After 5-Aza treatment, these cells expressed TbetaR-II at both mRNA and protein levels, which corresponded to the restoration of sensitivity to TGF-beta by an increase in P-Smad2. Levels of TGF-beta1 were similar before and after 5-Aza treatment. Results of the present study indicated that, in Renca cells, the loss of sensitivity to TGF-beta is likely due to a promoter hypermethylation in the TbetaR-II gene.

Authors+Show Affiliations

Department of Urology, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue. Tarry 16-733, Chicago, IL, 60611, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15620579

Citation

Zhang, Qiang, et al. "Restoration of Expression of Transforming Growth Factor-beta Type II Receptor in Murine Renal Cell Carcinoma (renca) Cells By 5-Aza-2'-deoxycytidine." Life Sciences, vol. 76, no. 10, 2005, pp. 1159-66.
Zhang Q, Rubenstein JN, Liu VC, et al. Restoration of expression of transforming growth factor-beta type II receptor in murine renal cell carcinoma (renca) cells by 5-Aza-2'-deoxycytidine. Life Sci. 2005;76(10):1159-66.
Zhang, Q., Rubenstein, J. N., Liu, V. C., Park, I., Jang, T., & Lee, C. (2005). Restoration of expression of transforming growth factor-beta type II receptor in murine renal cell carcinoma (renca) cells by 5-Aza-2'-deoxycytidine. Life Sciences, 76(10), 1159-66.
Zhang Q, et al. Restoration of Expression of Transforming Growth Factor-beta Type II Receptor in Murine Renal Cell Carcinoma (renca) Cells By 5-Aza-2'-deoxycytidine. Life Sci. 2005 Jan 21;76(10):1159-66. PubMed PMID: 15620579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Restoration of expression of transforming growth factor-beta type II receptor in murine renal cell carcinoma (renca) cells by 5-Aza-2'-deoxycytidine. AU - Zhang,Qiang, AU - Rubenstein,Jonathan N, AU - Liu,Victoria C, AU - Park,Irwin, AU - Jang,Thomas, AU - Lee,Chung, PY - 2004/07/29/received PY - 2004/10/12/accepted PY - 2004/12/29/pubmed PY - 2005/2/4/medline PY - 2004/12/29/entrez SP - 1159 EP - 66 JF - Life sciences JO - Life Sci VL - 76 IS - 10 N2 - The murine renal cell carcinoma (Renca) cells are insensitive to TGF-beta due to a lack of TGF-beta type II receptor (TbetaR-II). The objective of the present study is to determine the mechanism of this loss of sensitivity to TGF-beta in Renca cells. Renca cells were cultured and treated with 5-Aza-2'-Deoxycytidine (5-Aza), a specific inhibitor of methylation. Expression of TGF-beta type I receptor (TbetaRI) and TbetaRII was determined by RT-PCR and Western blot analysis before and after the treatment of Renca cells with 5-Aza. The expression of phosphorylated Smad2 (P-Smad2) was determined by Western blot analysis. TGF-beta levels in the conditioned medium were measured by ELISA. Renca cells did not express TbetaR-II prior to 5-Aza treatment. After 5-Aza treatment, these cells expressed TbetaR-II at both mRNA and protein levels, which corresponded to the restoration of sensitivity to TGF-beta by an increase in P-Smad2. Levels of TGF-beta1 were similar before and after 5-Aza treatment. Results of the present study indicated that, in Renca cells, the loss of sensitivity to TGF-beta is likely due to a promoter hypermethylation in the TbetaR-II gene. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15620579/Restoration_of_expression_of_transforming_growth_factor_beta_type_II_receptor_in_murine_renal_cell_carcinoma__renca__cells_by_5_Aza_2'_deoxycytidine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(04)00946-4 DB - PRIME DP - Unbound Medicine ER -