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Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels.

Abstract

Hydrogels with tissue-like mechanical properties are highly attractive scaffolds for tissue engineering. In this study, copolymers containing vinyl phosphonic acid (VPA) and acrylamide (AM) were tested for their swelling, protein uptake in serum supplemented medium, and cell adhesion and proliferation. The swelling of the gels in serum containing culture medium increased with increasing VPA content. The presence of VPA also increased protein uptake of gels in medium; gels polymerized with more than 50% of VPA absorbed as much as 100 microg/cm(2) of protein, twice the amount absorbed by gels made with only acrylamide. The adhesion and growth of the three types of cells, NIH 3T3 fibroblast, osteoblast-like MG-63 and Saos-2, were significantly improved on the gels made with 50% or more VPA; the number of adherent Mg-63 cells increased three-fold while the growth rate increased four-fold. Similar results were obtained for Saos-2 and 3T3 cells. The adhesion and growth of the three cell types on gels with sufficient phosphonate content were at least comparable to, or even better than, that on commercially available tissue culture plates. These results suggest great potential of anionic gels in bone tissue engineering.

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  • Publisher Full Text
  • Authors

    , , ,

    Source

    Biomaterials 26:17 2005 Jun pg 3663-71

    MeSH

    Absorbable Implants
    Absorption
    Acrylamide
    Animals
    Biocompatible Materials
    Cell Adhesion
    Cell Line
    Cell Proliferation
    Humans
    Hydrogels
    Materials Testing
    Mice
    NIH 3T3 Cells
    Organophosphonates
    Osteoblasts
    Protein Binding
    Serum
    Tissue Engineering
    Vinyl Compounds

    Pub Type(s)

    Comparative Study
    Evaluation Studies
    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    15621257

    Citation

    TY - JOUR T1 - Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels. AU - Tan,Jian, AU - Gemeinhart,Richard A, AU - Ma,Mandy, AU - Saltzman,W Mark, PY - 2004/Jun/28/received PY - 2004/Sep/22/accepted PY - 2004/12/29/pubmed PY - 2005/6/21/medline PY - 2004/12/29/entrez SP - 3663 EP - 71 JF - Biomaterials JO - Biomaterials VL - 26 IS - 17 N2 - Hydrogels with tissue-like mechanical properties are highly attractive scaffolds for tissue engineering. In this study, copolymers containing vinyl phosphonic acid (VPA) and acrylamide (AM) were tested for their swelling, protein uptake in serum supplemented medium, and cell adhesion and proliferation. The swelling of the gels in serum containing culture medium increased with increasing VPA content. The presence of VPA also increased protein uptake of gels in medium; gels polymerized with more than 50% of VPA absorbed as much as 100 microg/cm(2) of protein, twice the amount absorbed by gels made with only acrylamide. The adhesion and growth of the three types of cells, NIH 3T3 fibroblast, osteoblast-like MG-63 and Saos-2, were significantly improved on the gels made with 50% or more VPA; the number of adherent Mg-63 cells increased three-fold while the growth rate increased four-fold. Similar results were obtained for Saos-2 and 3T3 cells. The adhesion and growth of the three cell types on gels with sufficient phosphonate content were at least comparable to, or even better than, that on commercially available tissue culture plates. These results suggest great potential of anionic gels in bone tissue engineering. SN - 0142-9612 UR - https://www.unboundmedicine.com/medline/citation/15621257/Improved_cell_adhesion_and_proliferation_on_synthetic_phosphonic_acid_containing_hydrogels_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S0142-9612(04)00880-4 ER -