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Xanthine oxido-reductase activity in ischemic human and rat intestine.
Free Radic Res. 2004 Sep; 38(9):919-25.FR

Abstract

We measured time course and extent of xanthine dehydrogenase (XD) to xanthine oxidase (XO) conversion in ischemic human and rat intestine. To model normothermic no-flow ischemia, we incubated fresh biopsies for 0, 2, 4, 8 and 16h. At t = 0h, XO was less in humans than in rats (P < 0.0004), while XD was essentially the same (P = NS). After 16h incubation at 37 degrees C, there was no appreciable XD-to-XO conversion and no change in neither XO nor XD activity in human intestine. In contrast, the rat intestine had XO/(XO + XD) ratio doubled in the first 2h and then maintained that value until t = 16 h. In conclusion, no XO-to-XD conversion was appreciable after 16 h no-flow normothermic ischemia in human intestine; in contrast, XO activity in rats increased sharply after the onset of ischemia. An immunohistochemical labelling study shows that, whereas XO + XD expression in liver tissue is localised in both hepatocytes and endothelial cells, in the intestine that expression is mostly localised in epithelial cells. We conclude that XO may be considered as a major source of reactive oxygen species in rats but not in humans.

Authors+Show Affiliations

Dipartimento di Medicina, Chirurgia e Odontoiatria, University of Milan, San Paolo Hospital, via di Rudini' 8-20142 Milano, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15621709

Citation

Bianciardi, Paola, et al. "Xanthine Oxido-reductase Activity in Ischemic Human and Rat Intestine." Free Radical Research, vol. 38, no. 9, 2004, pp. 919-25.
Bianciardi P, Scorza R, Ghilardi G, et al. Xanthine oxido-reductase activity in ischemic human and rat intestine. Free Radic Res. 2004;38(9):919-25.
Bianciardi, P., Scorza, R., Ghilardi, G., & Samaja, M. (2004). Xanthine oxido-reductase activity in ischemic human and rat intestine. Free Radical Research, 38(9), 919-25.
Bianciardi P, et al. Xanthine Oxido-reductase Activity in Ischemic Human and Rat Intestine. Free Radic Res. 2004;38(9):919-25. PubMed PMID: 15621709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xanthine oxido-reductase activity in ischemic human and rat intestine. AU - Bianciardi,Paola, AU - Scorza,Roberto, AU - Ghilardi,Giorgio, AU - Samaja,Michele, PY - 2004/12/29/pubmed PY - 2005/9/29/medline PY - 2004/12/29/entrez SP - 919 EP - 25 JF - Free radical research JO - Free Radic Res VL - 38 IS - 9 N2 - We measured time course and extent of xanthine dehydrogenase (XD) to xanthine oxidase (XO) conversion in ischemic human and rat intestine. To model normothermic no-flow ischemia, we incubated fresh biopsies for 0, 2, 4, 8 and 16h. At t = 0h, XO was less in humans than in rats (P < 0.0004), while XD was essentially the same (P = NS). After 16h incubation at 37 degrees C, there was no appreciable XD-to-XO conversion and no change in neither XO nor XD activity in human intestine. In contrast, the rat intestine had XO/(XO + XD) ratio doubled in the first 2h and then maintained that value until t = 16 h. In conclusion, no XO-to-XD conversion was appreciable after 16 h no-flow normothermic ischemia in human intestine; in contrast, XO activity in rats increased sharply after the onset of ischemia. An immunohistochemical labelling study shows that, whereas XO + XD expression in liver tissue is localised in both hepatocytes and endothelial cells, in the intestine that expression is mostly localised in epithelial cells. We conclude that XO may be considered as a major source of reactive oxygen species in rats but not in humans. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/15621709/Xanthine_oxido_reductase_activity_in_ischemic_human_and_rat_intestine_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715760412331273430 DB - PRIME DP - Unbound Medicine ER -