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Nitric oxide and fetal organ blood flow during normoxia and hypoxemia in endotoxin-treated fetal sheep.
Obstet Gynecol. 2005 Jan; 105(1):145-55.OG

Abstract

OBJECTIVE

To investigate the role of nitric oxide in the process of circulatory decentralization during fetal hypoxemia.

METHODS

Fifteen sheep with singleton pregnancies were chronically instrumented at 107 days of gestation (term is 147 days). Three days later, 8 of the fetuses received nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthesis. Fifteen minutes after L-NAME administration, all 15 fetuses received lipopolysaccharides (LPS) from a strain of Escherichia coli. The 7 fetuses that received LPS only were used as controls. Sixty minutes after LPS was administered, the maternal aorta was occluded for 2 minutes in all fetuses. Organ blood flow and physiological variables were measured at 75 minutes before the start of occlusion (ie, at the time of L-NAME administration to the experimental group), at 1 minute before the start of occlusion, and at 2, 4, and 30 minutes after the start of occlusion.

RESULTS

Arterial pH was lower in the L-NAME group than in the control group at 1 minute before and 2 minutes after occlusion. Mean arterial pressure was higher in the L-NAME group than in the control group at 2 and 4 minutes after occlusion. Cardiac output fell in the L-NAME group and was lower than in the control group; the percentage of cardiac output to the cerebrum in the L-NAME group was 35% lower than that in the control group. Throughout the study, placental blood flow decreased by more than 80% in both groups and remained low. Blood flow to the fetal body decreased by 65% in the L-NAME group and was lower than in the control group. Blood flow to the carcass also decreased in the L-NAME group and was 36% of that in the control group.

CONCLUSION

Inhibition of nitric oxide synthesis causes a general vasoconstriction in practically all organs and leads to a reduction in LPS-induced circulatory decentralization. The changes in blood flow distribution in endotoxin-treated fetal sheep seem to be mediated in part by nitric oxide.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, University of Maastricht, Maastricht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15625156

Citation

Coumans, Audrey B C., et al. "Nitric Oxide and Fetal Organ Blood Flow During Normoxia and Hypoxemia in Endotoxin-treated Fetal Sheep." Obstetrics and Gynecology, vol. 105, no. 1, 2005, pp. 145-55.
Coumans AB, Garnier Y, Supçun S, et al. Nitric oxide and fetal organ blood flow during normoxia and hypoxemia in endotoxin-treated fetal sheep. Obstet Gynecol. 2005;105(1):145-55.
Coumans, A. B., Garnier, Y., Supçun, S., Jensen, A., Berger, R., & Hasaart, T. H. (2005). Nitric oxide and fetal organ blood flow during normoxia and hypoxemia in endotoxin-treated fetal sheep. Obstetrics and Gynecology, 105(1), 145-55.
Coumans AB, et al. Nitric Oxide and Fetal Organ Blood Flow During Normoxia and Hypoxemia in Endotoxin-treated Fetal Sheep. Obstet Gynecol. 2005;105(1):145-55. PubMed PMID: 15625156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide and fetal organ blood flow during normoxia and hypoxemia in endotoxin-treated fetal sheep. AU - Coumans,Audrey B C, AU - Garnier,Yves, AU - Supçun,Sirma, AU - Jensen,Arne, AU - Berger,Richard, AU - Hasaart,Tom H M, PY - 2004/12/31/pubmed PY - 2005/2/9/medline PY - 2004/12/31/entrez SP - 145 EP - 55 JF - Obstetrics and gynecology JO - Obstet Gynecol VL - 105 IS - 1 N2 - OBJECTIVE: To investigate the role of nitric oxide in the process of circulatory decentralization during fetal hypoxemia. METHODS: Fifteen sheep with singleton pregnancies were chronically instrumented at 107 days of gestation (term is 147 days). Three days later, 8 of the fetuses received nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthesis. Fifteen minutes after L-NAME administration, all 15 fetuses received lipopolysaccharides (LPS) from a strain of Escherichia coli. The 7 fetuses that received LPS only were used as controls. Sixty minutes after LPS was administered, the maternal aorta was occluded for 2 minutes in all fetuses. Organ blood flow and physiological variables were measured at 75 minutes before the start of occlusion (ie, at the time of L-NAME administration to the experimental group), at 1 minute before the start of occlusion, and at 2, 4, and 30 minutes after the start of occlusion. RESULTS: Arterial pH was lower in the L-NAME group than in the control group at 1 minute before and 2 minutes after occlusion. Mean arterial pressure was higher in the L-NAME group than in the control group at 2 and 4 minutes after occlusion. Cardiac output fell in the L-NAME group and was lower than in the control group; the percentage of cardiac output to the cerebrum in the L-NAME group was 35% lower than that in the control group. Throughout the study, placental blood flow decreased by more than 80% in both groups and remained low. Blood flow to the fetal body decreased by 65% in the L-NAME group and was lower than in the control group. Blood flow to the carcass also decreased in the L-NAME group and was 36% of that in the control group. CONCLUSION: Inhibition of nitric oxide synthesis causes a general vasoconstriction in practically all organs and leads to a reduction in LPS-induced circulatory decentralization. The changes in blood flow distribution in endotoxin-treated fetal sheep seem to be mediated in part by nitric oxide. SN - 0029-7844 UR - https://www.unboundmedicine.com/medline/citation/15625156/Nitric_oxide_and_fetal_organ_blood_flow_during_normoxia_and_hypoxemia_in_endotoxin_treated_fetal_sheep_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=15625156.ui DB - PRIME DP - Unbound Medicine ER -