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A high density microelectrode array biosensor for detection of E. coli O157:H7.
Biosens Bioelectron. 2005 Feb 15; 20(8):1662-7.BB

Abstract

A high density microelectrode array biosensor was developed for the detection of Escherichia coli O157:H7. The biosensor was fabricated from (100) silicon with a 2 microm layer of thermal oxide as an insulating layer, an active area of 9.6 mm2 and consists of an interdigitated gold electrode array. The sensor surface was functionalised for bacterial detection using heterobifunctional crosslinkers and immobilised polyclonal antibodies to create a biological sensing surface. Bacteria suspended in solution became attached to the immobilised antibodies when the biosensor was tested in liquid samples. The change in impedance caused by the bacteria was measured over a frequency range of 100 Hz-10 M Hz. The biosensor was evaluated for E. coli O157:H7 detection in pure culture and inoculated food samples. The biosensor was able to discriminate between cellular concentrations of 10(4)-10(7)CFU/mL and has applications in detecting pathogens in food samples.

Authors+Show Affiliations

Biosystems and Agricultural Engineering, Michigan State University, 204 Farrall Hall, East Lansing, MI 48823, USA.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15626625

Citation

Radke, Stephen M., and Evangelyn C. Alocilja. "A High Density Microelectrode Array Biosensor for Detection of E. Coli O157:H7." Biosensors & Bioelectronics, vol. 20, no. 8, 2005, pp. 1662-7.
Radke SM, Alocilja EC. A high density microelectrode array biosensor for detection of E. coli O157:H7. Biosens Bioelectron. 2005;20(8):1662-7.
Radke, S. M., & Alocilja, E. C. (2005). A high density microelectrode array biosensor for detection of E. coli O157:H7. Biosensors & Bioelectronics, 20(8), 1662-7.
Radke SM, Alocilja EC. A High Density Microelectrode Array Biosensor for Detection of E. Coli O157:H7. Biosens Bioelectron. 2005 Feb 15;20(8):1662-7. PubMed PMID: 15626625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A high density microelectrode array biosensor for detection of E. coli O157:H7. AU - Radke,Stephen M, AU - Alocilja,Evangelyn C, PY - 2004/05/24/received PY - 2004/07/17/revised PY - 2004/07/22/accepted PY - 2005/1/1/pubmed PY - 2005/6/10/medline PY - 2005/1/1/entrez SP - 1662 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 8 N2 - A high density microelectrode array biosensor was developed for the detection of Escherichia coli O157:H7. The biosensor was fabricated from (100) silicon with a 2 microm layer of thermal oxide as an insulating layer, an active area of 9.6 mm2 and consists of an interdigitated gold electrode array. The sensor surface was functionalised for bacterial detection using heterobifunctional crosslinkers and immobilised polyclonal antibodies to create a biological sensing surface. Bacteria suspended in solution became attached to the immobilised antibodies when the biosensor was tested in liquid samples. The change in impedance caused by the bacteria was measured over a frequency range of 100 Hz-10 M Hz. The biosensor was evaluated for E. coli O157:H7 detection in pure culture and inoculated food samples. The biosensor was able to discriminate between cellular concentrations of 10(4)-10(7)CFU/mL and has applications in detecting pathogens in food samples. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15626625/A_high_density_microelectrode_array_biosensor_for_detection_of_E__coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -