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[Effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress].
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2004 Oct; 30(5):569-76.ZW

Abstract

Effects of nitric oxide (NO), a substance newly found to have protective functions in plants, on root growth of wheat (Triticum aestivum L. Yangmai 158) seedlings under salt stress were studied. Sodium nitroprusside (SNP), an NO donor, markedly alleviated the inhibitory effect of salt on root elongation at salt concentrations around 150 mmol/L, but was ineffective when NaCl concentration was at 300 mmol/L or higher. It was most effective at 0.05-0.1 mmol/L, and had harmful effect at 0.30-5 mmol/L. Furthermore, when wheat seedling roots were treated with 150 mmol/L NaCl in combination with 0.05 mmol/L SNP and NO scavenger (hemoglobin, Hb), NO(-)(2)/NO(-)(3) and K(3)Fe(CN)(6) as controls, both of which were the two other major products besides NO when SNP dissolved in water, respectively, activities of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) were enhanced to some extent. While, the generation of O(-.)(2) and the accumulation of MDA and H(2)O(2) were alleviated. DNA laddering was observed when wheat seedling roots exposed to 150 mmol/L NaCl for 6 d, also suggesting that salt stress might induce oxidative damage in root-tips. In combination with hemoglobin, NO(-)(2)/NO(-)(3) and K(3)Fe(CN)(6) as controls, the results showed that NO could block DNA ladders. Above results suggest that it is NO rather than any substances which is protecting root-tip cells of wheat seedlings from oxidative damage caused by salt stress.

Authors+Show Affiliations

Chen M
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Shen WB
No affiliation info available
Ruan HH
No affiliation info available
Xu LL
No affiliation info available

MeSH

ApoptosisDNA FragmentationHydrogen PeroxideMalondialdehydeNitric OxideNitroprussideOxidation-ReductionPlant RootsSeedlingsSodium ChlorideSuperoxidesTriticum

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15627712

Citation

Chen, Ming, et al. "[Effects of Nitric Oxide On Root Growth and Its Oxidative Damage in Wheat Seedling Under Salt Stress]." Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology, vol. 30, no. 5, 2004, pp. 569-76.
Chen M, Shen WB, Ruan HH, et al. [Effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2004;30(5):569-76.
Chen, M., Shen, W. B., Ruan, H. H., & Xu, L. L. (2004). [Effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology, 30(5), 569-76.
Chen M, et al. [Effects of Nitric Oxide On Root Growth and Its Oxidative Damage in Wheat Seedling Under Salt Stress]. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2004;30(5):569-76. PubMed PMID: 15627712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of nitric oxide on root growth and its oxidative damage in wheat seedling under salt stress]. AU - Chen,Ming, AU - Shen,Wen-Biao, AU - Ruan,Hai-Hua, AU - Xu,Lang-Lai, PY - 2005/1/4/pubmed PY - 2005/1/28/medline PY - 2005/1/4/entrez SP - 569 EP - 76 JF - Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology JO - Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao VL - 30 IS - 5 N2 - Effects of nitric oxide (NO), a substance newly found to have protective functions in plants, on root growth of wheat (Triticum aestivum L. Yangmai 158) seedlings under salt stress were studied. Sodium nitroprusside (SNP), an NO donor, markedly alleviated the inhibitory effect of salt on root elongation at salt concentrations around 150 mmol/L, but was ineffective when NaCl concentration was at 300 mmol/L or higher. It was most effective at 0.05-0.1 mmol/L, and had harmful effect at 0.30-5 mmol/L. Furthermore, when wheat seedling roots were treated with 150 mmol/L NaCl in combination with 0.05 mmol/L SNP and NO scavenger (hemoglobin, Hb), NO(-)(2)/NO(-)(3) and K(3)Fe(CN)(6) as controls, both of which were the two other major products besides NO when SNP dissolved in water, respectively, activities of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) were enhanced to some extent. While, the generation of O(-.)(2) and the accumulation of MDA and H(2)O(2) were alleviated. DNA laddering was observed when wheat seedling roots exposed to 150 mmol/L NaCl for 6 d, also suggesting that salt stress might induce oxidative damage in root-tips. In combination with hemoglobin, NO(-)(2)/NO(-)(3) and K(3)Fe(CN)(6) as controls, the results showed that NO could block DNA ladders. Above results suggest that it is NO rather than any substances which is protecting root-tip cells of wheat seedlings from oxidative damage caused by salt stress. SN - 1671-3877 UR - https://www.unboundmedicine.com/medline/citation/15627712/[Effects_of_nitric_oxide_on_root_growth_and_its_oxidative_damage_in_wheat_seedling_under_salt_stress]_ DB - PRIME DP - Unbound Medicine ER -