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[Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Jan; 21(1):50-2.XB

Abstract

AIM

To express nucleocapsid(N) protein of SARS coronavirus and produce monoclonal antibody(mAb) to N protein.

METHODS

N protein gene was amplified by RT-PCR. After being confirmed by DNA sequencing, the gene was subcloned into prokaryotic expression vector. N protein expressed in E.coli was recovered from SDS-PAGE gel and served as immunogen in the preparation of the mAb.

RESULTS

DNA sequencing confirmed that the amplified fragment was N protein gene. SDS-PAGE analysis showed that the M(r) of the expressed protein was approximately 43 kd. This expressed protein could be further confirmed in Western blot by using serum from a convalescent SARS patient as primary antibody. Western blot analysis proved that three mAbs obtained could react specifically to the recombinant N protein.

CONCLUSION

The prepared recombinant N protein and mAbs against N protein lay the foundation for further development of early diagnosis assays for SARS coronavirus infection.

Authors+Show Affiliations

Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

15629083

Citation

Wang, Ping, et al. "[Preparation of Specific Monoclonal Antibody Against Nucleocapsid Protein of SARS Coronavirus]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 21, no. 1, 2005, pp. 50-2.
Wang P, Huang QS, Xue XP, et al. [Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005;21(1):50-2.
Wang, P., Huang, Q. S., Xue, X. P., Lei, Y. F., Wang, H. T., Ren, J. P., Ding, T. B., & Xu, Z. K. (2005). [Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 21(1), 50-2.
Wang P, et al. [Preparation of Specific Monoclonal Antibody Against Nucleocapsid Protein of SARS Coronavirus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005;21(1):50-2. PubMed PMID: 15629083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus]. AU - Wang,Ping, AU - Huang,Qing-Sheng, AU - Xue,Xiao-Ping, AU - Lei,Ying-Feng, AU - Wang,Hai-Tao, AU - Ren,Jun-Ping, AU - Ding,Tian-Bing, AU - Xu,Zhi-Kai, PY - 2005/1/5/pubmed PY - 2009/4/25/medline PY - 2005/1/5/entrez SP - 50 EP - 2 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 21 IS - 1 N2 - AIM: To express nucleocapsid(N) protein of SARS coronavirus and produce monoclonal antibody(mAb) to N protein. METHODS: N protein gene was amplified by RT-PCR. After being confirmed by DNA sequencing, the gene was subcloned into prokaryotic expression vector. N protein expressed in E.coli was recovered from SDS-PAGE gel and served as immunogen in the preparation of the mAb. RESULTS: DNA sequencing confirmed that the amplified fragment was N protein gene. SDS-PAGE analysis showed that the M(r) of the expressed protein was approximately 43 kd. This expressed protein could be further confirmed in Western blot by using serum from a convalescent SARS patient as primary antibody. Western blot analysis proved that three mAbs obtained could react specifically to the recombinant N protein. CONCLUSION: The prepared recombinant N protein and mAbs against N protein lay the foundation for further development of early diagnosis assays for SARS coronavirus infection. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/15629083/[Preparation_of_specific_monoclonal_antibody_against_nucleocapsid_protein_of_SARS_coronavirus]_ L2 - https://www.diseaseinfosearch.org/result/6415 DB - PRIME DP - Unbound Medicine ER -