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Anti-mitochondrial antibody IgG subclass distribution and affinity in primary biliary cirrhosis.
Clin Exp Immunol. 1992 Apr; 88(1):56-61.CE

Abstract

We have studied the total IgG subclass and anti-mitochondrial antibody (AMA) specific IgG subclass distribution in primary biliary cirrhosis (PBC) sera. In order to solve the problems caused by the differing affinities of subclass specific monoclonals and the competitive inhibition of antibodies in a whole serum assay, six sera were separated into subclass-specific fractions by affinity-depletion chromatography. AMA subclass distribution of 20 further sera from patients with PBC was assessed using conventional methods and the results were calibrated against one of the fractionated sera. Light chain distribution and AMA functional affinity were also assessed for the fractionated subclasses. Total amounts of IgG3 were significantly increased compared with normal controls. AMA were found in all IgG subclasses and not restricted predominantly to IgG3 as previously described. The functional affinity of IgG3 AMA is generally lower as compared with that of other subclasses. No light chain restriction was found.

Authors+Show Affiliations

Department of Medicine, School of Clinical Medicine, University of Cambridge, England.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1563107

Citation

Zhang, L, et al. "Anti-mitochondrial Antibody IgG Subclass Distribution and Affinity in Primary Biliary Cirrhosis." Clinical and Experimental Immunology, vol. 88, no. 1, 1992, pp. 56-61.
Zhang L, Weetman AP, Jayne DR, et al. Anti-mitochondrial antibody IgG subclass distribution and affinity in primary biliary cirrhosis. Clin Exp Immunol. 1992;88(1):56-61.
Zhang, L., Weetman, A. P., Jayne, D. R., Turner, I., Yeaman, S. J., Bassendine, M. F., & Oliveira, D. B. (1992). Anti-mitochondrial antibody IgG subclass distribution and affinity in primary biliary cirrhosis. Clinical and Experimental Immunology, 88(1), 56-61.
Zhang L, et al. Anti-mitochondrial Antibody IgG Subclass Distribution and Affinity in Primary Biliary Cirrhosis. Clin Exp Immunol. 1992;88(1):56-61. PubMed PMID: 1563107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-mitochondrial antibody IgG subclass distribution and affinity in primary biliary cirrhosis. AU - Zhang,L, AU - Weetman,A P, AU - Jayne,D R, AU - Turner,I, AU - Yeaman,S J, AU - Bassendine,M F, AU - Oliveira,D B, PY - 1992/4/1/pubmed PY - 1992/4/1/medline PY - 1992/4/1/entrez SP - 56 EP - 61 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 88 IS - 1 N2 - We have studied the total IgG subclass and anti-mitochondrial antibody (AMA) specific IgG subclass distribution in primary biliary cirrhosis (PBC) sera. In order to solve the problems caused by the differing affinities of subclass specific monoclonals and the competitive inhibition of antibodies in a whole serum assay, six sera were separated into subclass-specific fractions by affinity-depletion chromatography. AMA subclass distribution of 20 further sera from patients with PBC was assessed using conventional methods and the results were calibrated against one of the fractionated sera. Light chain distribution and AMA functional affinity were also assessed for the fractionated subclasses. Total amounts of IgG3 were significantly increased compared with normal controls. AMA were found in all IgG subclasses and not restricted predominantly to IgG3 as previously described. The functional affinity of IgG3 AMA is generally lower as compared with that of other subclasses. No light chain restriction was found. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/1563107/Anti_mitochondrial_antibody_IgG_subclass_distribution_and_affinity_in_primary_biliary_cirrhosis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0009-9104&date=1992&volume=88&issue=1&spage=56 DB - PRIME DP - Unbound Medicine ER -