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[Study on the response of specific antibodies against SARS-CoV in patients infected with SARS].
Zhonghua Liu Xing Bing Xue Za Zhi. 2004 Oct; 25(10):856-8.ZL

Abstract

OBJECTIVE

To study the response of specific antibodies against severe acute respiratory syndrome (SARS)-CoV in patients infected with SARS.

METHODS

IgM-capture, indirect and antigen-sandwiched enzyme linked immunosorbent assay (ELISA) were used to detect the SARS-CoV specific IgM, IgG and total antibodies in sera of clinical SARS patients or non-SARS individuals.

RESULTS

The positive rates of IgM, IgG and total antibodies to SARS-CoV in 146 sera of SARS patients collected in different phases of the disease were 61.64%, 53.43% and 69.86%, respectively. The earliest detectable days after onset of the disease for IgM and IgG to SRAS-CoV were 7 and 12 days, respectively. The specific IgM disappeared as early as 42 days after the onset of SARS. Of 70 sera from hepatitis A patients, 2 showed false positive results, while 127 sera from other patients were all negative, detected by the 3 methods. Serum from one medical worker who had been close contact to SARS patients was positive for anti-SARS-CoV IgG and total antibodies. These 3 methods used for detection were all not influenced by rheumatoid factor (RF).

CONCLUSION

All of the three methods were specific and sensitive for the detection of specific antibodies to SARS-CoV, and useful for epidemiological research and clinical diagnosis, but not for early diagnosis of SARS.

Authors+Show Affiliations

Mao PY
Institute of Infectious Diseases, Hospital 302 of the Chinese People's Liberation Army, Beijing 100039, China.
Zhu L
No affiliation info available
Wang YC
No affiliation info available
Hou J
No affiliation info available
Hu Y
No affiliation info available
Shen HH
No affiliation info available
Xin SJ
No affiliation info available
Li BS
No affiliation info available
Mao YL
No affiliation info available
Zhuang H
No affiliation info available

MeSH

Antibodies, ViralEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin GImmunoglobulin MMaleSevere acute respiratory syndrome-related coronavirusSensitivity and SpecificitySevere Acute Respiratory Syndrome

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15631740

Citation

Mao, Pan-yong, et al. "[Study On the Response of Specific Antibodies Against SARS-CoV in Patients Infected With SARS]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 25, no. 10, 2004, pp. 856-8.
Mao PY, Zhu L, Wang YC, et al. [Study on the response of specific antibodies against SARS-CoV in patients infected with SARS]. Zhonghua Liu Xing Bing Xue Za Zhi. 2004;25(10):856-8.
Mao, P. Y., Zhu, L., Wang, Y. C., Hou, J., Hu, Y., Shen, H. H., Xin, S. J., Li, B. S., Mao, Y. L., & Zhuang, H. (2004). [Study on the response of specific antibodies against SARS-CoV in patients infected with SARS]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 25(10), 856-8.
Mao PY, et al. [Study On the Response of Specific Antibodies Against SARS-CoV in Patients Infected With SARS]. Zhonghua Liu Xing Bing Xue Za Zhi. 2004;25(10):856-8. PubMed PMID: 15631740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on the response of specific antibodies against SARS-CoV in patients infected with SARS]. AU - Mao,Pan-yong, AU - Zhu,Lei, AU - Wang,You-chun, AU - Hou,Jun, AU - Hu,Yan, AU - Shen,Hong-hui, AU - Xin,Shao-jie, AU - Li,Bao-sen, AU - Mao,Yuan-li, AU - Zhuang,Hui, PY - 2005/1/6/pubmed PY - 2005/10/28/medline PY - 2005/1/6/entrez SP - 856 EP - 8 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 25 IS - 10 N2 - OBJECTIVE: To study the response of specific antibodies against severe acute respiratory syndrome (SARS)-CoV in patients infected with SARS. METHODS: IgM-capture, indirect and antigen-sandwiched enzyme linked immunosorbent assay (ELISA) were used to detect the SARS-CoV specific IgM, IgG and total antibodies in sera of clinical SARS patients or non-SARS individuals. RESULTS: The positive rates of IgM, IgG and total antibodies to SARS-CoV in 146 sera of SARS patients collected in different phases of the disease were 61.64%, 53.43% and 69.86%, respectively. The earliest detectable days after onset of the disease for IgM and IgG to SRAS-CoV were 7 and 12 days, respectively. The specific IgM disappeared as early as 42 days after the onset of SARS. Of 70 sera from hepatitis A patients, 2 showed false positive results, while 127 sera from other patients were all negative, detected by the 3 methods. Serum from one medical worker who had been close contact to SARS patients was positive for anti-SARS-CoV IgG and total antibodies. These 3 methods used for detection were all not influenced by rheumatoid factor (RF). CONCLUSION: All of the three methods were specific and sensitive for the detection of specific antibodies to SARS-CoV, and useful for epidemiological research and clinical diagnosis, but not for early diagnosis of SARS. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/15631740/[Study_on_the_response_of_specific_antibodies_against_SARS_CoV_in_patients_infected_with_SARS]_ DB - PRIME DP - Unbound Medicine ER -