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Adenosine triphosphatase activity of streptozotocin-induced diabetic rat brain microsomes. Effect of vitamin E.
Gen Physiol Biophys. 2004 Sep; 23(3):347-55.GP

Abstract

Hyperglycemia causes protein glycosylation, oxidation and alterations in enzyme activities, which are the underlying causes of diabetic complications. This study was undertaken to test the role of vitamin E treatment on Ca2+-ATPase activity, protein glycosylation and lipid peroxidation in the brain of streptozotocin (STZ)-induced diabetic rats. Male rats weighing about 250-300 g were rendered diabetic by a single STZ injection of 50 mg/kg via the tail vein. Both the diabetic and non-diabetic rats were fed a vitamin E supplemented diet (500 IU/kg/day). Ca2+-ATPase activity was significantly reduced at week 10 of diabetes compared to the control group (p < 0.05), with 0.225+/-0.021 U/I (mean +/- S.E.M.) in the control group and 0.072 +/- 0.008 U/l (mean +/- S.E.M.) in the diabetic group. Vitamin E treatment prevented the enzyme activity from decreasing. The activities observed were 0.226 +/- 0.020 U/l and 0.172 +/- 0.011 U/I (mean +/- S.E.M.) in the vitamin E-treated control and diabetic group, respectively. STZ-induced diabetes resulted in an increased protein glycosylation and lipid peroxidation. Vitamin E treatment led to a significant inhibition in blood glucose, protein glycosylation and lipid peroxidation, which in turn prevented abnormal activity of the enzyme in the brain. This study indicates that vitamin E supplementation may reduce complications of diabetes in the brain.

Authors+Show Affiliations

Ankara University, Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey. evcimen@pharmacy.ankara.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15638122

Citation

Das Evcimen, N, et al. "Adenosine Triphosphatase Activity of Streptozotocin-induced Diabetic Rat Brain Microsomes. Effect of Vitamin E." General Physiology and Biophysics, vol. 23, no. 3, 2004, pp. 347-55.
Das Evcimen N, Ulusu NN, Karasu C, et al. Adenosine triphosphatase activity of streptozotocin-induced diabetic rat brain microsomes. Effect of vitamin E. Gen Physiol Biophys. 2004;23(3):347-55.
Das Evcimen, N., Ulusu, N. N., Karasu, C., & Doğru, B. (2004). Adenosine triphosphatase activity of streptozotocin-induced diabetic rat brain microsomes. Effect of vitamin E. General Physiology and Biophysics, 23(3), 347-55.
Das Evcimen N, et al. Adenosine Triphosphatase Activity of Streptozotocin-induced Diabetic Rat Brain Microsomes. Effect of Vitamin E. Gen Physiol Biophys. 2004;23(3):347-55. PubMed PMID: 15638122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenosine triphosphatase activity of streptozotocin-induced diabetic rat brain microsomes. Effect of vitamin E. AU - Das Evcimen,N, AU - Ulusu,N N, AU - Karasu,C, AU - Doğru,B, PY - 2005/1/11/pubmed PY - 2005/3/23/medline PY - 2005/1/11/entrez SP - 347 EP - 55 JF - General physiology and biophysics JO - Gen Physiol Biophys VL - 23 IS - 3 N2 - Hyperglycemia causes protein glycosylation, oxidation and alterations in enzyme activities, which are the underlying causes of diabetic complications. This study was undertaken to test the role of vitamin E treatment on Ca2+-ATPase activity, protein glycosylation and lipid peroxidation in the brain of streptozotocin (STZ)-induced diabetic rats. Male rats weighing about 250-300 g were rendered diabetic by a single STZ injection of 50 mg/kg via the tail vein. Both the diabetic and non-diabetic rats were fed a vitamin E supplemented diet (500 IU/kg/day). Ca2+-ATPase activity was significantly reduced at week 10 of diabetes compared to the control group (p < 0.05), with 0.225+/-0.021 U/I (mean +/- S.E.M.) in the control group and 0.072 +/- 0.008 U/l (mean +/- S.E.M.) in the diabetic group. Vitamin E treatment prevented the enzyme activity from decreasing. The activities observed were 0.226 +/- 0.020 U/l and 0.172 +/- 0.011 U/I (mean +/- S.E.M.) in the vitamin E-treated control and diabetic group, respectively. STZ-induced diabetes resulted in an increased protein glycosylation and lipid peroxidation. Vitamin E treatment led to a significant inhibition in blood glucose, protein glycosylation and lipid peroxidation, which in turn prevented abnormal activity of the enzyme in the brain. This study indicates that vitamin E supplementation may reduce complications of diabetes in the brain. SN - 0231-5882 UR - https://www.unboundmedicine.com/medline/citation/15638122/Adenosine_triphosphatase_activity_of_streptozotocin_induced_diabetic_rat_brain_microsomes__Effect_of_vitamin_E_ L2 - http://www.gpb.sav.sk/2004_03_347.pdf DB - PRIME DP - Unbound Medicine ER -