Tags

Type your tag names separated by a space and hit enter

Immunoglobulin M memory B cell decrease in inflammatory bowel disease.
Eur Rev Med Pharmacol Sci 2004 Sep-Oct; 8(5):199-203ER

Abstract

BACKGROUND & OBJECTIVES

Memory B cells represent 30-60% of the B cell pool and can be subdivided in IgM memory and switched memory. IgM memory B cells differ from switched because they express IgM and their frequency may vary from 20-50% of the total memory pool. Switched memory express IgG, IgA or IgE and lack surface expression of IgM and IgD. Switched memory B cells derive from the germinal centres, whereas IgM memory B cells, which require the spleen for their survival and/or generation, are involved in the immune response to encapsulated bacteria. Since infections are one of the most frequent comorbid conditions in inflammatory bowel disease, we aimed to verify whether IgM memory B cell pool was decreased in Crohn's disease and ulcerative colitis patients.

PATIENTS & METHODS

Peripheral blood samples were obtained from 22 Crohn's disease patients, 20 ulcerative colitis patients, 22 healthy controls and 18 splenectomized patients. To analyse peripheral blood lymphocytes, flow cytometry was performed using anti-CD19, anti-CD22, anti-CD27, anti-IgM, anti-IgD and anti-CD38 monoclonal antibodies.

RESULTS

Circulating IgM memory B cells were significantly lower in Crohn's disease (median 7.1%, range 1.8-20.7) and ulcerative colitis patients (median 8.1%, range 2.1-18.8) in comparison to control subjects (median 14.0%, range 6.8-31.1). As expected, there was a highly significant difference in the proportion of IgM memory B cells between splenectomized patients (median 2.4%, range 0.9-6.9) and healthy controls. Crohn's disease patients with abscesses showed the lowest frequency of IgM memory B cells.

DISCUSSION

Our findings show that peripheral IgM memory B cells are reduced in inflammatory bowel disease patients. Further studies are necessary to answer the question of whether high risk of infection (abscess development) is promoted by the reduction/depletion of IgM memory B-cell pool in inflammatory bowel disease.

Authors+Show Affiliations

IRCCS Policlinico S Matteo, University of Pavia - Pavia (Italy).No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15638230

Citation

Di Sabatino, A, et al. "Immunoglobulin M Memory B Cell Decrease in Inflammatory Bowel Disease." European Review for Medical and Pharmacological Sciences, vol. 8, no. 5, 2004, pp. 199-203.
Di Sabatino A, Carsetti R, Rosado MM, et al. Immunoglobulin M memory B cell decrease in inflammatory bowel disease. Eur Rev Med Pharmacol Sci. 2004;8(5):199-203.
Di Sabatino, A., Carsetti, R., Rosado, M. M., Ciccocioppo, R., Cazzola, P., Morera, R., ... Corazza, G. R. (2004). Immunoglobulin M memory B cell decrease in inflammatory bowel disease. European Review for Medical and Pharmacological Sciences, 8(5), pp. 199-203.
Di Sabatino A, et al. Immunoglobulin M Memory B Cell Decrease in Inflammatory Bowel Disease. Eur Rev Med Pharmacol Sci. 2004;8(5):199-203. PubMed PMID: 15638230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoglobulin M memory B cell decrease in inflammatory bowel disease. AU - Di Sabatino,A, AU - Carsetti,R, AU - Rosado,M M, AU - Ciccocioppo,R, AU - Cazzola,P, AU - Morera,R, AU - Tinozzi,F P, AU - Tinozzi,S, AU - Corazza,G R, PY - 2005/1/11/pubmed PY - 2005/2/5/medline PY - 2005/1/11/entrez SP - 199 EP - 203 JF - European review for medical and pharmacological sciences JO - Eur Rev Med Pharmacol Sci VL - 8 IS - 5 N2 - BACKGROUND & OBJECTIVES: Memory B cells represent 30-60% of the B cell pool and can be subdivided in IgM memory and switched memory. IgM memory B cells differ from switched because they express IgM and their frequency may vary from 20-50% of the total memory pool. Switched memory express IgG, IgA or IgE and lack surface expression of IgM and IgD. Switched memory B cells derive from the germinal centres, whereas IgM memory B cells, which require the spleen for their survival and/or generation, are involved in the immune response to encapsulated bacteria. Since infections are one of the most frequent comorbid conditions in inflammatory bowel disease, we aimed to verify whether IgM memory B cell pool was decreased in Crohn's disease and ulcerative colitis patients. PATIENTS & METHODS: Peripheral blood samples were obtained from 22 Crohn's disease patients, 20 ulcerative colitis patients, 22 healthy controls and 18 splenectomized patients. To analyse peripheral blood lymphocytes, flow cytometry was performed using anti-CD19, anti-CD22, anti-CD27, anti-IgM, anti-IgD and anti-CD38 monoclonal antibodies. RESULTS: Circulating IgM memory B cells were significantly lower in Crohn's disease (median 7.1%, range 1.8-20.7) and ulcerative colitis patients (median 8.1%, range 2.1-18.8) in comparison to control subjects (median 14.0%, range 6.8-31.1). As expected, there was a highly significant difference in the proportion of IgM memory B cells between splenectomized patients (median 2.4%, range 0.9-6.9) and healthy controls. Crohn's disease patients with abscesses showed the lowest frequency of IgM memory B cells. DISCUSSION: Our findings show that peripheral IgM memory B cells are reduced in inflammatory bowel disease patients. Further studies are necessary to answer the question of whether high risk of infection (abscess development) is promoted by the reduction/depletion of IgM memory B-cell pool in inflammatory bowel disease. SN - 1128-3602 UR - https://www.unboundmedicine.com/medline/citation/15638230/Immunoglobulin_M_memory_B_cell_decrease_in_inflammatory_bowel_disease_ L2 - http://www.antibodies-online.com/pubmed/15638230 DB - PRIME DP - Unbound Medicine ER -