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Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats.
J Pharm Pharmacol. 2005 Jan; 57(1):145-50.JP

Abstract

This study was designed to examine the effects of aqueous garlic extract (AGE) on hepatic ischaemia-reperfusion (I/R) injury in rats. For this purpose, Wistar albino rats were subjected to 45 min of hepatic ischaemia, followed by a 60-min reperfusion period. AGE (1 mL kg(-1), i. p., corresponding to 500 mg kg(-1)) or saline was administered twice, 15 min before ischaemia and immediately before the reperfusion period. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined to assess liver functions. Liver tissues were taken for the determination of malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutathione (GSH) levels, a key antioxidant; and myeloperoxidase (MPO) activity, as an indirect index of neutrophil infiltration. Hepatic collagen content, as a fibrosis marker, was also determined. Plasma ALT and AST activities were elevated in the I/R group as compared with the control group, while these increases were significantly decreased by AGE treatment. Hepatic GSH levels, significantly depressed by I/R, were elevated back to control levels in the AGE-treated I/R group. Increases in tissue MDA levels and MPO activity due to I/R injury were reduced back to control levels by AGE treatment. Similarly, increased hepatic collagen content in the I/R group was reduced to the control level with AGE treatment. Since AGE administration alleviated the I/R-induced injury of the liver and improved the hepatic structure and function, it seems likely that AGE, with its antioxidant and oxidant-scavenging properties, may be of potential therapeutic value in protecting the liver against oxidative injury due to ischaemia-reperfusion.

Authors+Show Affiliations

Department of Pharmacology, Marmara University School of Pharmacy, Tibbiye Cad. 34668 Istanbul, Turkey. gokselsener@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15639002

Citation

Sener, Göksel, et al. "Aqueous Garlic Extract Alleviates Ischaemia-reperfusion-induced Oxidative Hepatic Injury in Rats." The Journal of Pharmacy and Pharmacology, vol. 57, no. 1, 2005, pp. 145-50.
Sener G, Sehirli O, Ipçi Y, et al. Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats. J Pharm Pharmacol. 2005;57(1):145-50.
Sener, G., Sehirli, O., Ipçi, Y., Ercan, F., Sirvanci, S., Gedik, N., & Yeğen, B. C. (2005). Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats. The Journal of Pharmacy and Pharmacology, 57(1), 145-50.
Sener G, et al. Aqueous Garlic Extract Alleviates Ischaemia-reperfusion-induced Oxidative Hepatic Injury in Rats. J Pharm Pharmacol. 2005;57(1):145-50. PubMed PMID: 15639002.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats. AU - Sener,Göksel, AU - Sehirli,Ozer, AU - Ipçi,Yeşim, AU - Ercan,Feriha, AU - Sirvanci,Serap, AU - Gedik,Nursal, AU - Yeğen,Berrak C, PY - 2005/1/11/pubmed PY - 2005/3/8/medline PY - 2005/1/11/entrez SP - 145 EP - 50 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 57 IS - 1 N2 - This study was designed to examine the effects of aqueous garlic extract (AGE) on hepatic ischaemia-reperfusion (I/R) injury in rats. For this purpose, Wistar albino rats were subjected to 45 min of hepatic ischaemia, followed by a 60-min reperfusion period. AGE (1 mL kg(-1), i. p., corresponding to 500 mg kg(-1)) or saline was administered twice, 15 min before ischaemia and immediately before the reperfusion period. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined to assess liver functions. Liver tissues were taken for the determination of malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutathione (GSH) levels, a key antioxidant; and myeloperoxidase (MPO) activity, as an indirect index of neutrophil infiltration. Hepatic collagen content, as a fibrosis marker, was also determined. Plasma ALT and AST activities were elevated in the I/R group as compared with the control group, while these increases were significantly decreased by AGE treatment. Hepatic GSH levels, significantly depressed by I/R, were elevated back to control levels in the AGE-treated I/R group. Increases in tissue MDA levels and MPO activity due to I/R injury were reduced back to control levels by AGE treatment. Similarly, increased hepatic collagen content in the I/R group was reduced to the control level with AGE treatment. Since AGE administration alleviated the I/R-induced injury of the liver and improved the hepatic structure and function, it seems likely that AGE, with its antioxidant and oxidant-scavenging properties, may be of potential therapeutic value in protecting the liver against oxidative injury due to ischaemia-reperfusion. SN - 0022-3573 UR - https://www.unboundmedicine.com/medline/citation/15639002/Aqueous_garlic_extract_alleviates_ischaemia_reperfusion_induced_oxidative_hepatic_injury_in_rats_ DB - PRIME DP - Unbound Medicine ER -