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Simultaneous determination of glycyrrhizin, a marker component in radix Glycyrrhizae, and its major metabolite glycyrrhetic acid in human plasma by LC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 25; 814(2):201-7.JC

Abstract

Glycyrrhizin (GLY) which has been widely used in traditional Chinese medicinal preparation possesses various pharmacological effects. In order to investigate the pharmacokinetic behavior of GLY in human after oral administration of GLY or licorice root, a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of GLY and its major metabolite glycyrrhetic acid (GA) in human plasma. The method involved a solid phase extraction of GLY, GA, and alpha-hederin, the internal standard (IS), from plasma with Waters Oasis MCX solid phase extraction (SPE) cartridges (30 mg) and a detection using a Micromass Quattro LC liquid chromatography/tandem mass spectrometry system with electrospray ionization source in positive ion mode. Separation of the analytes was achieved within 5min on a SepaxHP CN analytical column with a mobile phase of acetonitrile:water (50:50, v:v) containing 0.1% formic acid and 5mM ammonium acetate. Multiple reaction monitoring (MRM) was utilized for the detection monitoring 823--> 453 for GLY, 471--> 177 for GA and 752--> 456 for IS. The LC-MS/MS method was validated for specificity, sensitivity, accuracy, precision, and calibration function. The assay had a calibration range from 10 to 10,000 ng/mL and a lower limit of quantification of 10 ng/mL for both GLY and GA when 0.2 mL plasma was used for extraction. The percent coefficient of variation for accuracy and precision (inter-run and intra-run) for this method was less than 11.0% with a %Nominal ranging from 87.6 to 106.4% for GLY and 93.7 to 107.8% for GA. Stability of the analytes over sample processing (freeze/thaw, bench-top and long-term storage) and in the extracted samples was also tested and established.

Authors+Show Affiliations

Avantix Laboratories Inc., 57 Read's Way, New Castle, DE 19720, USA. linj@avantixlabs.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

15639440

Citation

Lin, Zhongping John, et al. "Simultaneous Determination of Glycyrrhizin, a Marker Component in Radix Glycyrrhizae, and Its Major Metabolite Glycyrrhetic Acid in Human Plasma By LC-MS/MS." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 814, no. 2, 2005, pp. 201-7.
Lin ZJ, Qiu SX, Wufuer A, et al. Simultaneous determination of glycyrrhizin, a marker component in radix Glycyrrhizae, and its major metabolite glycyrrhetic acid in human plasma by LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;814(2):201-7.
Lin, Z. J., Qiu, S. X., Wufuer, A., & Shum, L. (2005). Simultaneous determination of glycyrrhizin, a marker component in radix Glycyrrhizae, and its major metabolite glycyrrhetic acid in human plasma by LC-MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 814(2), 201-7.
Lin ZJ, et al. Simultaneous Determination of Glycyrrhizin, a Marker Component in Radix Glycyrrhizae, and Its Major Metabolite Glycyrrhetic Acid in Human Plasma By LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 25;814(2):201-7. PubMed PMID: 15639440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of glycyrrhizin, a marker component in radix Glycyrrhizae, and its major metabolite glycyrrhetic acid in human plasma by LC-MS/MS. AU - Lin,Zhongping John, AU - Qiu,Sheng-Xiang, AU - Wufuer,Aisya, AU - Shum,Linyee, PY - 2004/08/25/received PY - 2004/10/08/accepted PY - 2005/1/11/pubmed PY - 2005/5/19/medline PY - 2005/1/11/entrez SP - 201 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 814 IS - 2 N2 - Glycyrrhizin (GLY) which has been widely used in traditional Chinese medicinal preparation possesses various pharmacological effects. In order to investigate the pharmacokinetic behavior of GLY in human after oral administration of GLY or licorice root, a liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of GLY and its major metabolite glycyrrhetic acid (GA) in human plasma. The method involved a solid phase extraction of GLY, GA, and alpha-hederin, the internal standard (IS), from plasma with Waters Oasis MCX solid phase extraction (SPE) cartridges (30 mg) and a detection using a Micromass Quattro LC liquid chromatography/tandem mass spectrometry system with electrospray ionization source in positive ion mode. Separation of the analytes was achieved within 5min on a SepaxHP CN analytical column with a mobile phase of acetonitrile:water (50:50, v:v) containing 0.1% formic acid and 5mM ammonium acetate. Multiple reaction monitoring (MRM) was utilized for the detection monitoring 823--> 453 for GLY, 471--> 177 for GA and 752--> 456 for IS. The LC-MS/MS method was validated for specificity, sensitivity, accuracy, precision, and calibration function. The assay had a calibration range from 10 to 10,000 ng/mL and a lower limit of quantification of 10 ng/mL for both GLY and GA when 0.2 mL plasma was used for extraction. The percent coefficient of variation for accuracy and precision (inter-run and intra-run) for this method was less than 11.0% with a %Nominal ranging from 87.6 to 106.4% for GLY and 93.7 to 107.8% for GA. Stability of the analytes over sample processing (freeze/thaw, bench-top and long-term storage) and in the extracted samples was also tested and established. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15639440/Simultaneous_determination_of_glycyrrhizin_a_marker_component_in_radix_Glycyrrhizae_and_its_major_metabolite_glycyrrhetic_acid_in_human_plasma_by_LC_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00819-0 DB - PRIME DP - Unbound Medicine ER -