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[Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain].

Abstract

OBJECTIVE

To study the antigenicity of SARS associated coronavirus (CoV) spike S1 (12-672Aa) domain.

METHODS

BALB/c mice were immunized with a plasmid bearing codon-optimized SARS-CoV (Tor2 strain) S1 domain and then boosted with purified S1 protein; the SARS-CoV specific IgG antibody was tested by ELISA and neutralization antibody was determined by in vitro microneutralization assay.

RESULTS

S1 domain of SARS-CoV spike, which has been demonstrated harboring the receptor binding domain, successfully elicited SARS-CoV specific IgG antibody in mouse after combined immunization with DNA and purified S1 protein; the antibody elicited solely by S1 could potently neutralize SARS-CoV (HKU-39849) in vitro, 50% of 1 000 TCID50 SARS-CoV challenged cells were protected from viral infection by a 1:1499.68 dilution of mice sera immunized with S1 protein, but negative control sera showed no protection.

CONCLUSION

S1 domain of SARS-CoV spike protein, which is responsible for receptor binding, can efficiently and sufficiently induce highly potent neutralizing antibody in mice. This result suggested that S1 domain could be an effective subunit vaccines against SARS-CoV.

Authors+Show Affiliations

Department of Etiology, Institute of Basic Medical Science, CAMS and PUMC, Beijing 100005, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15640862

Citation

Zhang, Yun, et al. "[Potent Neutralization Antibody Elicited in Mice By SARS-associated Coronavirus Spike Protein S1 Domain]." Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, vol. 18, no. 3, 2004, pp. 258-60.
Zhang Y, Yang F, Li YH, et al. [Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004;18(3):258-60.
Zhang, Y., Yang, F., Li, Y. H., Li, W. H., Tu, X. M., Wei, Q., Zhu, H., Liu, L., Wang, H., Qin, C., Yuan, G. Y., He, W., & Wang, S. H. (2004). [Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, 18(3), 258-60.
Zhang Y, et al. [Potent Neutralization Antibody Elicited in Mice By SARS-associated Coronavirus Spike Protein S1 Domain]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004;18(3):258-60. PubMed PMID: 15640862.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain]. AU - Zhang,Yun, AU - Yang,Fan, AU - Li,Yan-han, AU - Li,Wen-hui, AU - Tu,Xin-ming, AU - Wei,Qiang, AU - Zhu,Hua, AU - Liu,Li, AU - Wang,Heng, AU - Qin,Chuan, AU - Yuan,Guo-yong, AU - He,Wei, AU - Wang,Shu-hui, PY - 2005/1/11/pubmed PY - 2006/8/9/medline PY - 2005/1/11/entrez SP - 258 EP - 60 JF - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology JO - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi VL - 18 IS - 3 N2 - OBJECTIVE: To study the antigenicity of SARS associated coronavirus (CoV) spike S1 (12-672Aa) domain. METHODS: BALB/c mice were immunized with a plasmid bearing codon-optimized SARS-CoV (Tor2 strain) S1 domain and then boosted with purified S1 protein; the SARS-CoV specific IgG antibody was tested by ELISA and neutralization antibody was determined by in vitro microneutralization assay. RESULTS: S1 domain of SARS-CoV spike, which has been demonstrated harboring the receptor binding domain, successfully elicited SARS-CoV specific IgG antibody in mouse after combined immunization with DNA and purified S1 protein; the antibody elicited solely by S1 could potently neutralize SARS-CoV (HKU-39849) in vitro, 50% of 1 000 TCID50 SARS-CoV challenged cells were protected from viral infection by a 1:1499.68 dilution of mice sera immunized with S1 protein, but negative control sera showed no protection. CONCLUSION: S1 domain of SARS-CoV spike protein, which is responsible for receptor binding, can efficiently and sufficiently induce highly potent neutralizing antibody in mice. This result suggested that S1 domain could be an effective subunit vaccines against SARS-CoV. SN - 1003-9279 UR - https://www.unboundmedicine.com/medline/citation/15640862/[Potent_neutralization_antibody_elicited_in_mice_by_SARS_associated_coronavirus_spike_protein_S1_domain]_ L2 - http://www.diseaseinfosearch.org/result/6415 DB - PRIME DP - Unbound Medicine ER -