TY - JOUR T1 - [Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis]. AU - Li,Zhuo, AU - Hao,Wa, AU - Lan,Hai-yun, AU - Gu,Wen-jie, AU - Wang,You-chun, PY - 2005/1/11/pubmed PY - 2006/8/9/medline PY - 2005/1/11/entrez SP - 268 EP - 71 JF - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology JO - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi VL - 18 IS - 3 N2 - OBJECTIVE: To investigate the anti hepatitis E virus (HEV) and HEV RNA in acute and convalescent sera of patients with NonA-E acute hepatitis. METHODS: The serum samples were taken from 95 patients who were diagnosed as acute NonA-E hepatitis. Enzyme immunoassay (EIA) was used for detecting anti-HEV Immunoglobulin G (IgG, Genolable and Wantai EIA anti-HEV kits). RT-PCR amplification of HEV RNA was based on the open reading frame 2 region of HEV and the PCR products were sequenced. RESULTS: Sera from 95 patients who were negative for anti-HEV in acute phase were followed up for 11-35 days to detect the anti-HEV antibody in recovery phase, 16/95 (16.84%) were positive for anti-HEV (wantai EIA anti-HEV kits). Ten (62.50%) were positive for HEV RNA in acute phase. Sequence analysis showed that 4 were HEV genotype. 6 were HEV genotype; 12/95 (12.50%) were positive for anti-HEV (Genolable EIA anti-HEV kits). Seven were positive for HEV RNA; 4 belonged to HEV genotype, 3 were HEV genotype. CONCLUSION: It is significant and necessary to detect anti HEV antibody and HEV RNA in patients with HEV infection during acute phase and convalesent phase. SN - 1003-9279 UR - https://www.unboundmedicine.com/medline/citation/15640865/[Clinical_significance_of_detecting_RNA_and_anti_HEV_antibody_in_convalesent_sera_in_patients_with_acute_HEV_hepatitis]_ L2 - https://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -