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[Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis].

Abstract

OBJECTIVE

To investigate the anti hepatitis E virus (HEV) and HEV RNA in acute and convalescent sera of patients with NonA-E acute hepatitis.

METHODS

The serum samples were taken from 95 patients who were diagnosed as acute NonA-E hepatitis. Enzyme immunoassay (EIA) was used for detecting anti-HEV Immunoglobulin G (IgG, Genolable and Wantai EIA anti-HEV kits). RT-PCR amplification of HEV RNA was based on the open reading frame 2 region of HEV and the PCR products were sequenced.

RESULTS

Sera from 95 patients who were negative for anti-HEV in acute phase were followed up for 11-35 days to detect the anti-HEV antibody in recovery phase, 16/95 (16.84%) were positive for anti-HEV (wantai EIA anti-HEV kits). Ten (62.50%) were positive for HEV RNA in acute phase. Sequence analysis showed that 4 were HEV genotype. 6 were HEV genotype; 12/95 (12.50%) were positive for anti-HEV (Genolable EIA anti-HEV kits). Seven were positive for HEV RNA; 4 belonged to HEV genotype, 3 were HEV genotype.

CONCLUSION

It is significant and necessary to detect anti HEV antibody and HEV RNA in patients with HEV infection during acute phase and convalesent phase.

Authors+Show Affiliations

Beijing You An hospital Affiliated to Capital University of Medical Sciences, Beijing 100054, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15640865

Citation

Li, Zhuo, et al. "[Clinical Significance of Detecting RNA and Anti HEV Antibody in Convalesent Sera in Patients With Acute HEV Hepatitis]." Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, vol. 18, no. 3, 2004, pp. 268-71.
Li Z, Hao W, Lan HY, et al. [Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004;18(3):268-71.
Li, Z., Hao, W., Lan, H. Y., Gu, W. J., & Wang, Y. C. (2004). [Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, 18(3), 268-71.
Li Z, et al. [Clinical Significance of Detecting RNA and Anti HEV Antibody in Convalesent Sera in Patients With Acute HEV Hepatitis]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004;18(3):268-71. PubMed PMID: 15640865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Clinical significance of detecting RNA and anti HEV antibody in convalesent sera in patients with acute HEV hepatitis]. AU - Li,Zhuo, AU - Hao,Wa, AU - Lan,Hai-yun, AU - Gu,Wen-jie, AU - Wang,You-chun, PY - 2005/1/11/pubmed PY - 2006/8/9/medline PY - 2005/1/11/entrez SP - 268 EP - 71 JF - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology JO - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi VL - 18 IS - 3 N2 - OBJECTIVE: To investigate the anti hepatitis E virus (HEV) and HEV RNA in acute and convalescent sera of patients with NonA-E acute hepatitis. METHODS: The serum samples were taken from 95 patients who were diagnosed as acute NonA-E hepatitis. Enzyme immunoassay (EIA) was used for detecting anti-HEV Immunoglobulin G (IgG, Genolable and Wantai EIA anti-HEV kits). RT-PCR amplification of HEV RNA was based on the open reading frame 2 region of HEV and the PCR products were sequenced. RESULTS: Sera from 95 patients who were negative for anti-HEV in acute phase were followed up for 11-35 days to detect the anti-HEV antibody in recovery phase, 16/95 (16.84%) were positive for anti-HEV (wantai EIA anti-HEV kits). Ten (62.50%) were positive for HEV RNA in acute phase. Sequence analysis showed that 4 were HEV genotype. 6 were HEV genotype; 12/95 (12.50%) were positive for anti-HEV (Genolable EIA anti-HEV kits). Seven were positive for HEV RNA; 4 belonged to HEV genotype, 3 were HEV genotype. CONCLUSION: It is significant and necessary to detect anti HEV antibody and HEV RNA in patients with HEV infection during acute phase and convalesent phase. SN - 1003-9279 UR - https://www.unboundmedicine.com/medline/citation/15640865/[Clinical_significance_of_detecting_RNA_and_anti_HEV_antibody_in_convalesent_sera_in_patients_with_acute_HEV_hepatitis]_ L2 - https://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -