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A structural pathway for signaling in the E46Q mutant of photoactive yellow protein.
Structure. 2005 Jan; 13(1):55-63.S

Abstract

In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15642261

Citation

Rajagopal, Sudarshan, et al. "A Structural Pathway for Signaling in the E46Q Mutant of Photoactive Yellow Protein." Structure (London, England : 1993), vol. 13, no. 1, 2005, pp. 55-63.
Rajagopal S, Anderson S, Srajer V, et al. A structural pathway for signaling in the E46Q mutant of photoactive yellow protein. Structure. 2005;13(1):55-63.
Rajagopal, S., Anderson, S., Srajer, V., Schmidt, M., Pahl, R., & Moffat, K. (2005). A structural pathway for signaling in the E46Q mutant of photoactive yellow protein. Structure (London, England : 1993), 13(1), 55-63.
Rajagopal S, et al. A Structural Pathway for Signaling in the E46Q Mutant of Photoactive Yellow Protein. Structure. 2005;13(1):55-63. PubMed PMID: 15642261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A structural pathway for signaling in the E46Q mutant of photoactive yellow protein. AU - Rajagopal,Sudarshan, AU - Anderson,Spencer, AU - Srajer,Vukica, AU - Schmidt,Marius, AU - Pahl,Reinhard, AU - Moffat,Keith, PY - 2004/07/22/received PY - 2004/10/21/revised PY - 2004/10/21/accepted PY - 2005/1/12/pubmed PY - 2005/5/20/medline PY - 2005/1/12/entrez SP - 55 EP - 63 JF - Structure (London, England : 1993) JO - Structure VL - 13 IS - 1 N2 - In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational change in the protein associated with differential signaling activity, as it executes a reversible photocycle. Time-resolved Laue crystallography allows structural snapshots (as short as 150 ps) of high crystallographic resolution (approximately 1.6 A) to be taken of a protein as it functions. Here, we analyze by singular value decomposition a comprehensive time-resolved crystallographic data set of the E46Q mutant of PYP throughout the photocycle spanning 10 ns-100 ms. We identify and refine the structures of five distinct intermediates and provide a plausible chemical kinetic mechanism for their inter conversion. A clear structural progression is visible in these intermediates, in which a signal generated at the chromophore propagates through a distinct structural pathway of conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore. SN - 0969-2126 UR - https://www.unboundmedicine.com/medline/citation/15642261/A_structural_pathway_for_signaling_in_the_E46Q_mutant_of_photoactive_yellow_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-2126(04)00390-9 DB - PRIME DP - Unbound Medicine ER -