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Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs.
J Endocrinol 2005; 184(1):179-89JE

Abstract

The IGF system is one of the most important endocrine and paracrine growth factor systems that regulate fetal and placental growth. We hypothesized that intrauterine growth restriction (IUGR) in guinea pigs is mediated by the altered expression of IGFs and/or IGF binding protein (BP) mRNAs in tissues and is related to growth of specific tissues. IUGR was induced by unilateral uterine artery ligation on day 30 of gestation, and fetal plasma, amniotic fluid and tissue samples were collected at 55-57 days (term about 68 days) from paired IUGR and control fetuses (n=6). Western ligand blotting and immunoblotting were used to compare IGFBP levels in plasma and amniotic fluid. Total RNA was extracted from placenta and fetal tissues, and the relative abundance of IGF-II and IGFBP-1-6 mRNA was determined by Northern blotting, using species-specific probes where available. IUGR fetuses had decreased (P<0.01, by Student's t-test) placental weight and body weight with an increase in the brain:liver weight ratio. The principal IGFBPs in fetal plasma migrated at 40-35, 30 and 25 kDa and were identified as IGFBP-3, -2 and -4 respectively. IUGR was associated with elevated plasma IGFBP-2 and IGFBP-4 and reduced IGFBP-3 levels. IGFBPs were detected at low levels in amniotic fluid of control fetuses but at higher levels in IUGR fetuses. In IUGR placentae, there was a small increase in IGFBP-4 mRNA (P<0.05). IGFBP-2 mRNA increased (P<0.001) in liver of IUGR fetuses. IGF-II and IGFBP mRNA expression did not change in fetal muscle. The results are consistent with reduced IGF action, directly or through inhibition by IGFBPs, particularly by circulating and tissue IGFBP-2, as a potential causal factor in decreased growth of the placenta and certain fetal tissues.

Authors+Show Affiliations

Child Health Research Institute, University of Western Ontario, London, Ontario N6C 2V5, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15642794

Citation

Carter, A M., et al. "Altered Expression of IGFs and IGF-binding Proteins During Intrauterine Growth Restriction in Guinea Pigs." The Journal of Endocrinology, vol. 184, no. 1, 2005, pp. 179-89.
Carter AM, Kingston MJ, Han KK, et al. Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs. J Endocrinol. 2005;184(1):179-89.
Carter, A. M., Kingston, M. J., Han, K. K., Mazzuca, D. M., Nygard, K., & Han, V. K. (2005). Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs. The Journal of Endocrinology, 184(1), pp. 179-89.
Carter AM, et al. Altered Expression of IGFs and IGF-binding Proteins During Intrauterine Growth Restriction in Guinea Pigs. J Endocrinol. 2005;184(1):179-89. PubMed PMID: 15642794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs. AU - Carter,A M, AU - Kingston,M J, AU - Han,K K, AU - Mazzuca,D M, AU - Nygard,K, AU - Han,V K M, PY - 2005/1/12/pubmed PY - 2005/2/26/medline PY - 2005/1/12/entrez SP - 179 EP - 89 JF - The Journal of endocrinology JO - J. Endocrinol. VL - 184 IS - 1 N2 - The IGF system is one of the most important endocrine and paracrine growth factor systems that regulate fetal and placental growth. We hypothesized that intrauterine growth restriction (IUGR) in guinea pigs is mediated by the altered expression of IGFs and/or IGF binding protein (BP) mRNAs in tissues and is related to growth of specific tissues. IUGR was induced by unilateral uterine artery ligation on day 30 of gestation, and fetal plasma, amniotic fluid and tissue samples were collected at 55-57 days (term about 68 days) from paired IUGR and control fetuses (n=6). Western ligand blotting and immunoblotting were used to compare IGFBP levels in plasma and amniotic fluid. Total RNA was extracted from placenta and fetal tissues, and the relative abundance of IGF-II and IGFBP-1-6 mRNA was determined by Northern blotting, using species-specific probes where available. IUGR fetuses had decreased (P<0.01, by Student's t-test) placental weight and body weight with an increase in the brain:liver weight ratio. The principal IGFBPs in fetal plasma migrated at 40-35, 30 and 25 kDa and were identified as IGFBP-3, -2 and -4 respectively. IUGR was associated with elevated plasma IGFBP-2 and IGFBP-4 and reduced IGFBP-3 levels. IGFBPs were detected at low levels in amniotic fluid of control fetuses but at higher levels in IUGR fetuses. In IUGR placentae, there was a small increase in IGFBP-4 mRNA (P<0.05). IGFBP-2 mRNA increased (P<0.001) in liver of IUGR fetuses. IGF-II and IGFBP mRNA expression did not change in fetal muscle. The results are consistent with reduced IGF action, directly or through inhibition by IGFBPs, particularly by circulating and tissue IGFBP-2, as a potential causal factor in decreased growth of the placenta and certain fetal tissues. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/15642794/Altered_expression_of_IGFs_and_IGF_binding_proteins_during_intrauterine_growth_restriction_in_guinea_pigs_ L2 - https://joe.bioscientifica.com/doi/10.1677/joe.1.05781 DB - PRIME DP - Unbound Medicine ER -