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Chemical and biological assessment of a traditional chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis: orthogonal array design to optimize the extraction of chemical constituents.
Planta Med. 2004 Dec; 70(12):1222-7.PM

Abstract

Danggui Buxue Tang (DBT) is a simple decoction, having about 800 years of usage in China to treat menopausal irregularity in women, which contains two herbs: Radix Astragali (Huangqi) and Radix Angelicae Sinensis (Danggui). Traditionally, boiling water has been used for preparing DBT; however, the optimized conditions of extraction have not yet been determined. Here, the amounts of Radix Astragali-derived astragaloside IV, calycosin, formononetin, and Radix Angelicae Sinensis-derived ferulic acid and ligustilide were determined in DBT, which were extracted according to an orthogonal array experimental design having three variable parameters: extraction time, extraction volume and number of repeats of the extraction. Our results suggest that extraction time and number of repeats of the extraction are two crucial factors, while extraction volume is a subordinate factor. The optimized conditions for extraction were therefore established. Besides the chemical composition, the estrogenic and anti-platelet aggregation activities of DBT were determined in different groups of the extraction, and the results of bioassays were in line with the amounts of the analyzed chemical ingredients. The results provide a model system for establishing the quality assurance of the herbal preparation.

Authors+Show Affiliations

Song ZH
Department of Biology, The Hong Kong University of Science and Technology, Hong Kong, China.
Ji ZN
No affiliation info available
Lo CK
No affiliation info available
Dong TT
No affiliation info available
Zhao KJ
No affiliation info available
Li OT
No affiliation info available
Haines CJ
No affiliation info available
Kung SD
No affiliation info available
Tsim KW
No affiliation info available

MeSH

Alkaline PhosphataseAngelicaAngelica sinensisAntineoplastic Agents, PhytogenicCell Line, TumorCell ProliferationDose-Response Relationship, DrugDrugs, Chinese HerbalHumansOsteosarcomaPhytotherapyPlant RootsPlatelet AggregationPlatelet Aggregation Inhibitors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15643561

Citation

Song, Zong H., et al. "Chemical and Biological Assessment of a Traditional Chinese Herbal Decoction Prepared From Radix Astragali and Radix Angelicae Sinensis: Orthogonal Array Design to Optimize the Extraction of Chemical Constituents." Planta Medica, vol. 70, no. 12, 2004, pp. 1222-7.
Song ZH, Ji ZN, Lo CK, et al. Chemical and biological assessment of a traditional chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis: orthogonal array design to optimize the extraction of chemical constituents. Planta Med. 2004;70(12):1222-7.
Song, Z. H., Ji, Z. N., Lo, C. K., Dong, T. T., Zhao, K. J., Li, O. T., Haines, C. J., Kung, S. D., & Tsim, K. W. (2004). Chemical and biological assessment of a traditional chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis: orthogonal array design to optimize the extraction of chemical constituents. Planta Medica, 70(12), 1222-7.
Song ZH, et al. Chemical and Biological Assessment of a Traditional Chinese Herbal Decoction Prepared From Radix Astragali and Radix Angelicae Sinensis: Orthogonal Array Design to Optimize the Extraction of Chemical Constituents. Planta Med. 2004;70(12):1222-7. PubMed PMID: 15643561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical and biological assessment of a traditional chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis: orthogonal array design to optimize the extraction of chemical constituents. AU - Song,Zong H, AU - Ji,Zhao N, AU - Lo,Chun K, AU - Dong,Tina T X, AU - Zhao,Kui J, AU - Li,Olive T W, AU - Haines,Christopher J, AU - Kung,Shain D, AU - Tsim,Karl W K, PY - 2005/1/12/pubmed PY - 2005/3/31/medline PY - 2005/1/12/entrez SP - 1222 EP - 7 JF - Planta medica JO - Planta Med VL - 70 IS - 12 N2 - Danggui Buxue Tang (DBT) is a simple decoction, having about 800 years of usage in China to treat menopausal irregularity in women, which contains two herbs: Radix Astragali (Huangqi) and Radix Angelicae Sinensis (Danggui). Traditionally, boiling water has been used for preparing DBT; however, the optimized conditions of extraction have not yet been determined. Here, the amounts of Radix Astragali-derived astragaloside IV, calycosin, formononetin, and Radix Angelicae Sinensis-derived ferulic acid and ligustilide were determined in DBT, which were extracted according to an orthogonal array experimental design having three variable parameters: extraction time, extraction volume and number of repeats of the extraction. Our results suggest that extraction time and number of repeats of the extraction are two crucial factors, while extraction volume is a subordinate factor. The optimized conditions for extraction were therefore established. Besides the chemical composition, the estrogenic and anti-platelet aggregation activities of DBT were determined in different groups of the extraction, and the results of bioassays were in line with the amounts of the analyzed chemical ingredients. The results provide a model system for establishing the quality assurance of the herbal preparation. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/15643561/Chemical_and_biological_assessment_of_a_traditional_chinese_herbal_decoction_prepared_from_Radix_Astragali_and_Radix_Angelicae_Sinensis:_orthogonal_array_design_to_optimize_the_extraction_of_chemical_constituents_ DB - PRIME DP - Unbound Medicine ER -
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