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Gene expression profiling of rat midbrain dopamine neurons: implications for selective vulnerability in parkinsonism.
Neurobiol Dis. 2005 Feb; 18(1):19-31.ND

Abstract

To elucidate factors related to selective dopamine neuron degeneration in Parkinson's disease (PD), we have defined gene expression profiles of discrete dopamine neuron subpopulations in the rat using immunofluorescent laser capture microscopy and microarray analysis. Although profiles were remarkably similar, there are concerted categorical differences in gene expression between dopamine neurons that might explain their differential susceptibility. As a group, energy metabolism transcripts are more highly expressed in substantia nigra (SN) dopamine neurons, an intriguing result considering previous evidence for a mitochondrial defect in idiopathic PD and the greater susceptibility of SN dopamine neurons to damage by mitochondrial poisons. Examination of putative transcription factor binding sites suggests that these concerted differences may be related to differential activity of specific transcription factors. These results provide the first large scale description of gene expression profiles of dopamine neurons and suggest several avenues for investigation into dopaminergic neuroprotective therapy for PD.

Authors+Show Affiliations

Department of Pharmacology, Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA. jggreen@emory.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15649693

Citation

Greene, James G., et al. "Gene Expression Profiling of Rat Midbrain Dopamine Neurons: Implications for Selective Vulnerability in Parkinsonism." Neurobiology of Disease, vol. 18, no. 1, 2005, pp. 19-31.
Greene JG, Dingledine R, Greenamyre JT. Gene expression profiling of rat midbrain dopamine neurons: implications for selective vulnerability in parkinsonism. Neurobiol Dis. 2005;18(1):19-31.
Greene, J. G., Dingledine, R., & Greenamyre, J. T. (2005). Gene expression profiling of rat midbrain dopamine neurons: implications for selective vulnerability in parkinsonism. Neurobiology of Disease, 18(1), 19-31.
Greene JG, Dingledine R, Greenamyre JT. Gene Expression Profiling of Rat Midbrain Dopamine Neurons: Implications for Selective Vulnerability in Parkinsonism. Neurobiol Dis. 2005;18(1):19-31. PubMed PMID: 15649693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression profiling of rat midbrain dopamine neurons: implications for selective vulnerability in parkinsonism. AU - Greene,James G, AU - Dingledine,Raymond, AU - Greenamyre,J Timothy, PY - 2004/07/12/received PY - 2004/10/06/revised PY - 2004/10/12/accepted PY - 2005/1/15/pubmed PY - 2005/5/17/medline PY - 2005/1/15/entrez SP - 19 EP - 31 JF - Neurobiology of disease JO - Neurobiol Dis VL - 18 IS - 1 N2 - To elucidate factors related to selective dopamine neuron degeneration in Parkinson's disease (PD), we have defined gene expression profiles of discrete dopamine neuron subpopulations in the rat using immunofluorescent laser capture microscopy and microarray analysis. Although profiles were remarkably similar, there are concerted categorical differences in gene expression between dopamine neurons that might explain their differential susceptibility. As a group, energy metabolism transcripts are more highly expressed in substantia nigra (SN) dopamine neurons, an intriguing result considering previous evidence for a mitochondrial defect in idiopathic PD and the greater susceptibility of SN dopamine neurons to damage by mitochondrial poisons. Examination of putative transcription factor binding sites suggests that these concerted differences may be related to differential activity of specific transcription factors. These results provide the first large scale description of gene expression profiles of dopamine neurons and suggest several avenues for investigation into dopaminergic neuroprotective therapy for PD. SN - 0969-9961 UR - https://www.unboundmedicine.com/medline/citation/15649693/Gene_expression_profiling_of_rat_midbrain_dopamine_neurons:_implications_for_selective_vulnerability_in_parkinsonism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-9961(04)00236-0 DB - PRIME DP - Unbound Medicine ER -