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Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans.
Mol Cell Probes. 2005 Feb; 19(1):1-8.MC

Abstract

A method for one-stage rapid identification of four orthopoxvirus species pathogenic to humans based on multiplex polymerase chain reaction (MPCR) was developed. Five pairs of oligonucleotide primers--one, genus-specific; and the rest, species-specific for variola, monkeypox, cowpox, and vaccinia viruses, respectively--were used concurrently for MPCR assay of orthopoxvirus DNAs. Specificity and sensitivity of the method developed were evaluated using DNAs of 57 orthopoxvirus strains, including the DNAs isolated from human case clinical materials.

Authors+Show Affiliations

State Research Center of Virology and Biotechnology Vector, Koltsovo Novosibirsk Oblast, Koltsovo 630559, Russia. snshchel@vector.nsc.ruNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15652214

Citation

Shchelkunov, S N., et al. "Multiplex PCR Detection and Species Differentiation of Orthopoxviruses Pathogenic to Humans." Molecular and Cellular Probes, vol. 19, no. 1, 2005, pp. 1-8.
Shchelkunov SN, Gavrilova EV, Babkin IV. Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans. Mol Cell Probes. 2005;19(1):1-8.
Shchelkunov, S. N., Gavrilova, E. V., & Babkin, I. V. (2005). Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans. Molecular and Cellular Probes, 19(1), 1-8.
Shchelkunov SN, Gavrilova EV, Babkin IV. Multiplex PCR Detection and Species Differentiation of Orthopoxviruses Pathogenic to Humans. Mol Cell Probes. 2005;19(1):1-8. PubMed PMID: 15652214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans. AU - Shchelkunov,S N, AU - Gavrilova,E V, AU - Babkin,I V, PY - 2004/02/24/received PY - 2004/07/22/accepted PY - 2005/1/18/pubmed PY - 2005/7/1/medline PY - 2005/1/18/entrez SP - 1 EP - 8 JF - Molecular and cellular probes JO - Mol Cell Probes VL - 19 IS - 1 N2 - A method for one-stage rapid identification of four orthopoxvirus species pathogenic to humans based on multiplex polymerase chain reaction (MPCR) was developed. Five pairs of oligonucleotide primers--one, genus-specific; and the rest, species-specific for variola, monkeypox, cowpox, and vaccinia viruses, respectively--were used concurrently for MPCR assay of orthopoxvirus DNAs. Specificity and sensitivity of the method developed were evaluated using DNAs of 57 orthopoxvirus strains, including the DNAs isolated from human case clinical materials. SN - 0890-8508 UR - https://www.unboundmedicine.com/medline/citation/15652214/Multiplex_PCR_detection_and_species_differentiation_of_orthopoxviruses_pathogenic_to_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-8508(04)00072-6 DB - PRIME DP - Unbound Medicine ER -