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Effect of quercetine and glutathione on the level of superoxide dismutase, catalase, malonyldialdehyde, blood pressure and neonatal outcome in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl ester.
Eur J Obstet Gynecol Reprod Biol. 2005 Feb 01; 118(2):190-5.EJ

Abstract

OBJECTIVE

To anticipate the impact of antioxidant use on lipid peroxidation products, free oxygen radical scavengers, blood pressure (BP), proteinuria and neonatal outcome (as seen in percentage survival, litter birth weight) in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl-ester (L-NAME), an inhibitor of nitric oxide synthase (NOS).

MATERIAL AND METHODS

Female adult non-pregnant Sprague-Dawley rats (n=40) with timed pregnancies were allocated into four groups according to medication they received on day 17 to term. Rats were randomised into a sham-treated group (group I, n=10) and groups treated with L-NAME, 50 mg/day i.p., only (group II, n=10), L-NAME + quercetine, 10mg/kg i.p. (group III, n=10) and L-NAME + glutathione, 60 mg/kg i.p. (group IV, n=10). Blood levels of superoxide dismutase (SOD), catalase (CAT) and malonyldialdehyde (MDA) were assessed on day 22 of gestation. Intracardiac blood sampling and hysterotomy were performed on day 22 of gestation. Mean systolic BP (measured with a tail-cuff device), level of proteinuria, total urine output, pups birth weight and percentages of live and of dead pups were recorded.

RESULTS

Mean systolic BP and SOD, CAT and MDA levels were higher in rats infused with L-NAME than in the sham-treated group. In group IV, SOD levels were lower than in group II (P <0.001). A linear positive correlation between BPs on day 20 and SOD levels (rp=0.39) was recorded, as were negative correlations between level of proteinuria and SOD levels (rp=-0.39) and between CAT and MDA levels (rp=-0.39). Birth weights were higher in the sham-treated group than in the other groups (P <0.001). Pups of hypertensive gravid rats treated with antioxidants had better survival rates than those of rats in group II and the sham-treated group (Chi-square=15.9, d.f.: 3, P <0.01).However, no correlation was detected between higher pup mortality rate and birth weight of pups.

CONCLUSION

In this rat model of pre-eclampsia, adverse outcomes, such as proteinuria and high neonatal death rate, are reversed by exogenous antioxidant use, even though no significant improvement is detected in terms of BP and birth weight of pups.

Authors+Show Affiliations

Department of Obstetrics and Gynaecology, Osmangazi University Faculty of Medicine, Division of Maternal and Fetal Medicine, Meselik Kampusu 26480, Eskisehir, Turkey. mtanir@superonline.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15653201

Citation

Tanir, H Mete, et al. "Effect of Quercetine and Glutathione On the Level of Superoxide Dismutase, Catalase, Malonyldialdehyde, Blood Pressure and Neonatal Outcome in a Rat Model of Pre-eclampsia Induced By NG-nitro-L-arginine-methyl Ester." European Journal of Obstetrics, Gynecology, and Reproductive Biology, vol. 118, no. 2, 2005, pp. 190-5.
Tanir HM, Sener T, Inal M, et al. Effect of quercetine and glutathione on the level of superoxide dismutase, catalase, malonyldialdehyde, blood pressure and neonatal outcome in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl ester. Eur J Obstet Gynecol Reprod Biol. 2005;118(2):190-5.
Tanir, H. M., Sener, T., Inal, M., Akyuz, F., Uzuner, K., & Sivri, E. (2005). Effect of quercetine and glutathione on the level of superoxide dismutase, catalase, malonyldialdehyde, blood pressure and neonatal outcome in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl ester. European Journal of Obstetrics, Gynecology, and Reproductive Biology, 118(2), 190-5.
Tanir HM, et al. Effect of Quercetine and Glutathione On the Level of Superoxide Dismutase, Catalase, Malonyldialdehyde, Blood Pressure and Neonatal Outcome in a Rat Model of Pre-eclampsia Induced By NG-nitro-L-arginine-methyl Ester. Eur J Obstet Gynecol Reprod Biol. 2005 Feb 1;118(2):190-5. PubMed PMID: 15653201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of quercetine and glutathione on the level of superoxide dismutase, catalase, malonyldialdehyde, blood pressure and neonatal outcome in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl ester. AU - Tanir,H Mete, AU - Sener,Turgay, AU - Inal,Mine, AU - Akyuz,Fahrettin, AU - Uzuner,Kubilay, AU - Sivri,Ergul, PY - 2003/11/03/received PY - 2004/02/11/revised PY - 2004/04/11/accepted PY - 2005/1/18/pubmed PY - 2005/6/2/medline PY - 2005/1/18/entrez SP - 190 EP - 5 JF - European journal of obstetrics, gynecology, and reproductive biology JO - Eur J Obstet Gynecol Reprod Biol VL - 118 IS - 2 N2 - OBJECTIVE: To anticipate the impact of antioxidant use on lipid peroxidation products, free oxygen radical scavengers, blood pressure (BP), proteinuria and neonatal outcome (as seen in percentage survival, litter birth weight) in a rat model of pre-eclampsia induced by NG-nitro-L-arginine-methyl-ester (L-NAME), an inhibitor of nitric oxide synthase (NOS). MATERIAL AND METHODS: Female adult non-pregnant Sprague-Dawley rats (n=40) with timed pregnancies were allocated into four groups according to medication they received on day 17 to term. Rats were randomised into a sham-treated group (group I, n=10) and groups treated with L-NAME, 50 mg/day i.p., only (group II, n=10), L-NAME + quercetine, 10mg/kg i.p. (group III, n=10) and L-NAME + glutathione, 60 mg/kg i.p. (group IV, n=10). Blood levels of superoxide dismutase (SOD), catalase (CAT) and malonyldialdehyde (MDA) were assessed on day 22 of gestation. Intracardiac blood sampling and hysterotomy were performed on day 22 of gestation. Mean systolic BP (measured with a tail-cuff device), level of proteinuria, total urine output, pups birth weight and percentages of live and of dead pups were recorded. RESULTS: Mean systolic BP and SOD, CAT and MDA levels were higher in rats infused with L-NAME than in the sham-treated group. In group IV, SOD levels were lower than in group II (P <0.001). A linear positive correlation between BPs on day 20 and SOD levels (rp=0.39) was recorded, as were negative correlations between level of proteinuria and SOD levels (rp=-0.39) and between CAT and MDA levels (rp=-0.39). Birth weights were higher in the sham-treated group than in the other groups (P <0.001). Pups of hypertensive gravid rats treated with antioxidants had better survival rates than those of rats in group II and the sham-treated group (Chi-square=15.9, d.f.: 3, P <0.01).However, no correlation was detected between higher pup mortality rate and birth weight of pups. CONCLUSION: In this rat model of pre-eclampsia, adverse outcomes, such as proteinuria and high neonatal death rate, are reversed by exogenous antioxidant use, even though no significant improvement is detected in terms of BP and birth weight of pups. SN - 0301-2115 UR - https://www.unboundmedicine.com/medline/citation/15653201/Effect_of_quercetine_and_glutathione_on_the_level_of_superoxide_dismutase_catalase_malonyldialdehyde_blood_pressure_and_neonatal_outcome_in_a_rat_model_of_pre_eclampsia_induced_by_NG_nitro_L_arginine_methyl_ester_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301211504002702 DB - PRIME DP - Unbound Medicine ER -