Tags

Type your tag names separated by a space and hit enter

Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves.
Plant Cell Physiol. 2004 Dec; 45(12):1738-48.PC

Abstract

Poplar, whose genome is the first to be sequenced among woody plants, is a favorable model for plant biologists to enable them to understand molecular processes of growth, development and responses to environmental stimuli in trees. The sequence will allow the development of a strategy for improving environmental stress tolerance in forest trees. In this study, we have generated a full-length enriched cDNA library from leaves of axenically grown poplar (Populus nigra var. italica) subjected to environmental stress treatments by dehydration, high salinity, chilling, heat, abscisic acid (ABA) and H2O2. We sequenced >30,000 expressed sequence tags (ESTs) from the cDNA library and consequently collected approximately 4,500 non-redundant clones. We further analyzed cDNAs encoding an ERF/AP2-domain transcription factor which is specific in plants and plays an important role under stress. Thirteen candidates containing the ERF/AP2 domain were found within our EST resource. Some of them showed stress-responsive gene expression. We report here the first collection of full-length enriched stress-related ESTs of poplar and discuss environmental stress responses of forest trees in the light of comparative genomics.

Authors+Show Affiliations

Nanjo T
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687 Japan. nanjo@affrc.go.jp
Futamura N
No affiliation info available
Nishiguchi M
No affiliation info available
Igasaki T
No affiliation info available
Shinozaki K
No affiliation info available
Shinohara K
No affiliation info available

MeSH

Abscisic AcidDNA, ComplementaryDehydrationExpressed Sequence TagsGene Expression Regulation, PlantGene LibraryGenes, PlantGenome, PlantMolecular Sequence DataOxidantsPhylogenyPlant LeavesPlant ProteinsPopulusProtein Structure, TertiarySequence Homology, Amino AcidSequence Homology, Nucleic AcidSodium ChlorideTemperatureTranscription Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15653793

Citation

Nanjo, Tokihiko, et al. "Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves." Plant & Cell Physiology, vol. 45, no. 12, 2004, pp. 1738-48.
Nanjo T, Futamura N, Nishiguchi M, et al. Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves. Plant Cell Physiol. 2004;45(12):1738-48.
Nanjo, T., Futamura, N., Nishiguchi, M., Igasaki, T., Shinozaki, K., & Shinohara, K. (2004). Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves. Plant & Cell Physiology, 45(12), 1738-48.
Nanjo T, et al. Characterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves. Plant Cell Physiol. 2004;45(12):1738-48. PubMed PMID: 15653793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of full-length enriched expressed sequence tags of stress-treated poplar leaves. AU - Nanjo,Tokihiko, AU - Futamura,Norihiro, AU - Nishiguchi,Mitsuru, AU - Igasaki,Tomohiro, AU - Shinozaki,Kazuo, AU - Shinohara,Kenji, PY - 2005/1/18/pubmed PY - 2005/6/11/medline PY - 2005/1/18/entrez SP - 1738 EP - 48 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 45 IS - 12 N2 - Poplar, whose genome is the first to be sequenced among woody plants, is a favorable model for plant biologists to enable them to understand molecular processes of growth, development and responses to environmental stimuli in trees. The sequence will allow the development of a strategy for improving environmental stress tolerance in forest trees. In this study, we have generated a full-length enriched cDNA library from leaves of axenically grown poplar (Populus nigra var. italica) subjected to environmental stress treatments by dehydration, high salinity, chilling, heat, abscisic acid (ABA) and H2O2. We sequenced >30,000 expressed sequence tags (ESTs) from the cDNA library and consequently collected approximately 4,500 non-redundant clones. We further analyzed cDNAs encoding an ERF/AP2-domain transcription factor which is specific in plants and plays an important role under stress. Thirteen candidates containing the ERF/AP2 domain were found within our EST resource. Some of them showed stress-responsive gene expression. We report here the first collection of full-length enriched stress-related ESTs of poplar and discuss environmental stress responses of forest trees in the light of comparative genomics. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/15653793/Characterization_of_full_length_enriched_expressed_sequence_tags_of_stress_treated_poplar_leaves_ DB - PRIME DP - Unbound Medicine ER -