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Effects of renal nerve stimulation on intrarenal blood flow in rats with intact or inactivated NO synthases.
Acta Physiol Scand. 2005 Jan; 183(1):99-105.AP

Abstract

AIM

We studied a possible action of nitric oxide (NO), an intrarenal vasodilator, to buffer a decrease in renal perfusion induced by electrical stimulation of renal nerves (RNS).

METHODS

In anaesthetized rats RNS was performed (15 V, 2 ms pulse duration) for 10 s at the frequencies of 2, 3.5, 5 and 7.5 Hz. The total renal blood flow (RBF), an index of cortical perfusion, was measured using a Transonic probe on the renal artery. The outer and inner medullary blood flow (OMBF, IMBF) was measured by laser-Doppler flowmetry. The effect of RNS on RBF, OMBF and IMBF was determined in rats which were either untreated or pre-treated with L-NAME (0.6 or 1.8 mg kg(-1) i.v.), or S-methyl thiocitrulline (SMTC, 20 microg kg(-1) min(-1) i.v.), a selective inhibitor of neuronal NO synthase (nNOS).

RESULTS

In untreated rats, RNS decreased IMBF significantly less than RBF and OMBF. High-dose L-NAME treatment significantly enhanced the RNS induced decrease of RBF but not of OMBF or IMBF. SMTC treatment significantly enhanced the decrease of IMBF, without affecting the response of RBF or OMBF.

CONCLUSION

At intact NO synthesis the inner medullary circulation is not controlled by renal nerves to the extent observed for the outer medulla or cortex. NO generated by all NOS isoforms present in the kidney buffers partly the intrarenal vasoconstriction triggered by electrical RNS. The NO derived from nNOS seems of particular importance in the control of inner medullary perfusion, interacting with NO generated by endothelial NOS and renal nerves.

Authors+Show Affiliations

Laboratory of Renal and Body Fluid Physiology, M. Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15654923

Citation

Walkowska, A, et al. "Effects of Renal Nerve Stimulation On Intrarenal Blood Flow in Rats With Intact or Inactivated NO Synthases." Acta Physiologica Scandinavica, vol. 183, no. 1, 2005, pp. 99-105.
Walkowska A, Badzyńska B, Kompanowska-Jezierska E, et al. Effects of renal nerve stimulation on intrarenal blood flow in rats with intact or inactivated NO synthases. Acta Physiol Scand. 2005;183(1):99-105.
Walkowska, A., Badzyńska, B., Kompanowska-Jezierska, E., Johns, E. J., & Sadowski, J. (2005). Effects of renal nerve stimulation on intrarenal blood flow in rats with intact or inactivated NO synthases. Acta Physiologica Scandinavica, 183(1), 99-105.
Walkowska A, et al. Effects of Renal Nerve Stimulation On Intrarenal Blood Flow in Rats With Intact or Inactivated NO Synthases. Acta Physiol Scand. 2005;183(1):99-105. PubMed PMID: 15654923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of renal nerve stimulation on intrarenal blood flow in rats with intact or inactivated NO synthases. AU - Walkowska,A, AU - Badzyńska,B, AU - Kompanowska-Jezierska,E, AU - Johns,E J, AU - Sadowski,J, PY - 2005/1/19/pubmed PY - 2005/5/4/medline PY - 2005/1/19/entrez SP - 99 EP - 105 JF - Acta physiologica Scandinavica JO - Acta Physiol Scand VL - 183 IS - 1 N2 - AIM: We studied a possible action of nitric oxide (NO), an intrarenal vasodilator, to buffer a decrease in renal perfusion induced by electrical stimulation of renal nerves (RNS). METHODS: In anaesthetized rats RNS was performed (15 V, 2 ms pulse duration) for 10 s at the frequencies of 2, 3.5, 5 and 7.5 Hz. The total renal blood flow (RBF), an index of cortical perfusion, was measured using a Transonic probe on the renal artery. The outer and inner medullary blood flow (OMBF, IMBF) was measured by laser-Doppler flowmetry. The effect of RNS on RBF, OMBF and IMBF was determined in rats which were either untreated or pre-treated with L-NAME (0.6 or 1.8 mg kg(-1) i.v.), or S-methyl thiocitrulline (SMTC, 20 microg kg(-1) min(-1) i.v.), a selective inhibitor of neuronal NO synthase (nNOS). RESULTS: In untreated rats, RNS decreased IMBF significantly less than RBF and OMBF. High-dose L-NAME treatment significantly enhanced the RNS induced decrease of RBF but not of OMBF or IMBF. SMTC treatment significantly enhanced the decrease of IMBF, without affecting the response of RBF or OMBF. CONCLUSION: At intact NO synthesis the inner medullary circulation is not controlled by renal nerves to the extent observed for the outer medulla or cortex. NO generated by all NOS isoforms present in the kidney buffers partly the intrarenal vasoconstriction triggered by electrical RNS. The NO derived from nNOS seems of particular importance in the control of inner medullary perfusion, interacting with NO generated by endothelial NOS and renal nerves. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/15654923/Effects_of_renal_nerve_stimulation_on_intrarenal_blood_flow_in_rats_with_intact_or_inactivated_NO_synthases_ DB - PRIME DP - Unbound Medicine ER -