Tags

Type your tag names separated by a space and hit enter

Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas.
World J Gastroenterol. 2005 Feb 07; 11(5):629-33.WJ

Abstract

AIM

To understand the expression of latent and lytic genes of Epstein-Barr virus (EBV) in EBV-associated gastric carcinoma (EBVaGC) and to explore the relationship between EBV-encoded genes and development of EBVaGC at molecular level.

METHODS

One hundred and seventy-two gastric carcinoma tissues and 172 corresponding para-carcinoma tissues were tested for EBV genome by polymerase chain reaction (PCR)-Southern blotting. EBV-encoded small RNA (EBER) 1 of the PCR positive specimens was detected by in situ hybridization (ISH). Gastric carcinomas with positive EBER1 signals were classified as EBVaGCs. RT-PCR and Southern hybridization were applied to the detection of expression of nuclear antigen (EBNA) promoters (Qp, Wp and Cp), EBNA 1 and EBNA 2, latent membrane proteins (LMP) 1, 2A and 2B and lytic genes (immediate early genes BZLF1 and BRLF1, early genes BARF1 and BHRF1, late genes BcLF1 and BLLF1) in EBVaGCs.

RESULTS

Eleven EBV positive samples existed in gastric carcinoma tissues (6.39%). No EBV positive sample was found in corresponding para-carcinoma tissues. The difference between EBV positivity in carcinoma tissues and corresponding para-carcinoma tissues was significant (chi(2) = 9.0909, P = 0.0026). Transcripts of Qp and EBNA1 were detected in all the 11 EBVaGCs, while both Wp and Cp were silent. EBNA2, LMP1 and LMP2B mRNA were absent in all the cases, while LMP2A mRNA was detected in 4 of the 11 cases. Of the 11 EBVaGCs, 7 exhibited BcLF1 transcripts and 2 exhibited BHRF1 transcripts. The transcripts of BZLF1 and BARF1 were detected in 5 cases, respectively. No BLLF1 and BRLF mRNA were detected.

CONCLUSION

The latent pattern of EBV in gastric carcinoma corresponds to the latency I/II. Some lytic infection genes are expressed in EBVaGCs tissues. BARF1 and BHRF1 genes may play an important role in tumorigenesis of gastric carcinoma.

Authors+Show Affiliations

Department of Microbiology, Qingdao University Medical College, Number 38 of Dengzhou Road, Qingdao 266021, Shandong Province, China. qingdaoluobing@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15655811

Citation

Luo, Bing, et al. "Expression of Epstein-Barr Virus Genes in EBV-associated Gastric Carcinomas." World Journal of Gastroenterology, vol. 11, no. 5, 2005, pp. 629-33.
Luo B, Wang Y, Wang XF, et al. Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas. World J Gastroenterol. 2005;11(5):629-33.
Luo, B., Wang, Y., Wang, X. F., Liang, H., Yan, L. P., Huang, B. H., & Zhao, P. (2005). Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas. World Journal of Gastroenterology, 11(5), 629-33.
Luo B, et al. Expression of Epstein-Barr Virus Genes in EBV-associated Gastric Carcinomas. World J Gastroenterol. 2005 Feb 7;11(5):629-33. PubMed PMID: 15655811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas. AU - Luo,Bing, AU - Wang,Yun, AU - Wang,Xiao-Feng, AU - Liang,Hua, AU - Yan,Li-Ping, AU - Huang,Bao-Hua, AU - Zhao,Peng, PY - 2005/1/19/pubmed PY - 2005/3/15/medline PY - 2005/1/19/entrez SP - 629 EP - 33 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 11 IS - 5 N2 - AIM: To understand the expression of latent and lytic genes of Epstein-Barr virus (EBV) in EBV-associated gastric carcinoma (EBVaGC) and to explore the relationship between EBV-encoded genes and development of EBVaGC at molecular level. METHODS: One hundred and seventy-two gastric carcinoma tissues and 172 corresponding para-carcinoma tissues were tested for EBV genome by polymerase chain reaction (PCR)-Southern blotting. EBV-encoded small RNA (EBER) 1 of the PCR positive specimens was detected by in situ hybridization (ISH). Gastric carcinomas with positive EBER1 signals were classified as EBVaGCs. RT-PCR and Southern hybridization were applied to the detection of expression of nuclear antigen (EBNA) promoters (Qp, Wp and Cp), EBNA 1 and EBNA 2, latent membrane proteins (LMP) 1, 2A and 2B and lytic genes (immediate early genes BZLF1 and BRLF1, early genes BARF1 and BHRF1, late genes BcLF1 and BLLF1) in EBVaGCs. RESULTS: Eleven EBV positive samples existed in gastric carcinoma tissues (6.39%). No EBV positive sample was found in corresponding para-carcinoma tissues. The difference between EBV positivity in carcinoma tissues and corresponding para-carcinoma tissues was significant (chi(2) = 9.0909, P = 0.0026). Transcripts of Qp and EBNA1 were detected in all the 11 EBVaGCs, while both Wp and Cp were silent. EBNA2, LMP1 and LMP2B mRNA were absent in all the cases, while LMP2A mRNA was detected in 4 of the 11 cases. Of the 11 EBVaGCs, 7 exhibited BcLF1 transcripts and 2 exhibited BHRF1 transcripts. The transcripts of BZLF1 and BARF1 were detected in 5 cases, respectively. No BLLF1 and BRLF mRNA were detected. CONCLUSION: The latent pattern of EBV in gastric carcinoma corresponds to the latency I/II. Some lytic infection genes are expressed in EBVaGCs tissues. BARF1 and BHRF1 genes may play an important role in tumorigenesis of gastric carcinoma. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/15655811/Expression_of_Epstein_Barr_virus_genes_in_EBV_associated_gastric_carcinomas_ L2 - http://www.wjgnet.com/1007-9327/full/v11/i5/629.htm DB - PRIME DP - Unbound Medicine ER -