[Determination of carvacrol and thymol in Mosla chinensis by HPLC].Zhongguo Zhong Yao Za Zhi. 2004 Nov; 29(11):1030-2.ZZ
To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis.
The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm.
The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%.
The method is reliable, and can be used for quality control of M. chinensis.