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[Simultaneous determination of 4 anthraquinones in Kuhuang injection by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2004 Nov; 29(11):1041-4.ZZ

Abstract

OBJECTIVE

To develop a new high-performance liquid chromatographic method for simultaneous quantitative determination of 4 anthraquinones in Kuhuang injection.

METHOD

The chromatographic separation was performed on a Lichrospher C18 column (4.6 mm x 250 mm, 5 microm,), A linear gradient elution of A (CH3COOH: H2O = 1:100) and B (CH3COOH: H2O: CH3CN = 1:20:80) was used. The flow rate was 0.8 mL x min(-1) and column temperature was set at 35 degrees C. The UV detection wavelength was set at 254 nm.

RESULT

The recoveries of rhein, emodin, chrysophanol and physcion were 98.9%, 100.5%, 102.5% and 99.0%, respectively. Their linear ranges were 0.0875-1.75 microg for rhein, 0.0825-1.65 microg for emodin, 0.159-3.17 microg for chrysophanol and 0.0525-1.05 microg for physcion, respectively.

CONCLUSION

The method was accurate, repeatable and suitable to determine the contents of 4 anthraquinones in Kuhuang injection.

Authors+Show Affiliations

Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15656132

Citation

Wu, Yong-jiang, et al. "[Simultaneous Determination of 4 Anthraquinones in Kuhuang Injection By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 29, no. 11, 2004, pp. 1041-4.
Wu YJ, Chen JJ, Cheng YY. [Simultaneous determination of 4 anthraquinones in Kuhuang injection by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2004;29(11):1041-4.
Wu, Y. J., Chen, J. J., & Cheng, Y. Y. (2004). [Simultaneous determination of 4 anthraquinones in Kuhuang injection by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 29(11), 1041-4.
Wu YJ, Chen JJ, Cheng YY. [Simultaneous Determination of 4 Anthraquinones in Kuhuang Injection By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2004;29(11):1041-4. PubMed PMID: 15656132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Simultaneous determination of 4 anthraquinones in Kuhuang injection by RP-HPLC]. AU - Wu,Yong-jiang, AU - Chen,Jian-jun, AU - Cheng,Yi-yu, PY - 2005/1/20/pubmed PY - 2005/6/4/medline PY - 2005/1/20/entrez SP - 1041 EP - 4 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 29 IS - 11 N2 - OBJECTIVE: To develop a new high-performance liquid chromatographic method for simultaneous quantitative determination of 4 anthraquinones in Kuhuang injection. METHOD: The chromatographic separation was performed on a Lichrospher C18 column (4.6 mm x 250 mm, 5 microm,), A linear gradient elution of A (CH3COOH: H2O = 1:100) and B (CH3COOH: H2O: CH3CN = 1:20:80) was used. The flow rate was 0.8 mL x min(-1) and column temperature was set at 35 degrees C. The UV detection wavelength was set at 254 nm. RESULT: The recoveries of rhein, emodin, chrysophanol and physcion were 98.9%, 100.5%, 102.5% and 99.0%, respectively. Their linear ranges were 0.0875-1.75 microg for rhein, 0.0825-1.65 microg for emodin, 0.159-3.17 microg for chrysophanol and 0.0525-1.05 microg for physcion, respectively. CONCLUSION: The method was accurate, repeatable and suitable to determine the contents of 4 anthraquinones in Kuhuang injection. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/15656132/[Simultaneous_determination_of_4_anthraquinones_in_Kuhuang_injection_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -