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RAD51-dependent break-induced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion.
Mol Cell Biol. 2005 Feb; 25(3):933-44.MC

Abstract

Diploid Saccharomyces cells experiencing a double-strand break (DSB) on one homologous chromosome repair the break by RAD51-mediated gene conversion >98% of the time. However, when extensive homologous sequences are restricted to one side of the DSB, repair can occur by both RAD51-dependent and RAD51-independent break-induced replication (BIR) mechanisms. Here we characterize the kinetics and checkpoint dependence of RAD51-dependent BIR when the DSB is created within a chromosome. Gene conversion products appear within 2 h, and there is little, if any, induction of the DNA damage checkpoint; however, RAD51-dependent BIR occurs with a further delay of 2 to 4 h and cells arrest in response to the G(2)/M DNA damage checkpoint. RAD51-dependent BIR does not require special facilitating sequences that are required for a less efficient RAD51-independent process. RAD51-dependent BIR occurs efficiently in G(2)-arrested cells. Once repair is initiated, the rate of repair replication during BIR is comparable to that of normal DNA replication, as copying of >100 kb is completed less than 30 min after repair DNA synthesis is detected close to the DSB.

Authors+Show Affiliations

Rosenstiel Center, Brandeis University, 415 South St., Mail Stop 029, Waltham, MA 02454-9110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15657422

Citation

Malkova, Anna, et al. "RAD51-dependent Break-induced Replication Differs in Kinetics and Checkpoint Responses From RAD51-mediated Gene Conversion." Molecular and Cellular Biology, vol. 25, no. 3, 2005, pp. 933-44.
Malkova A, Naylor ML, Yamaguchi M, et al. RAD51-dependent break-induced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion. Mol Cell Biol. 2005;25(3):933-44.
Malkova, A., Naylor, M. L., Yamaguchi, M., Ira, G., & Haber, J. E. (2005). RAD51-dependent break-induced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion. Molecular and Cellular Biology, 25(3), 933-44.
Malkova A, et al. RAD51-dependent Break-induced Replication Differs in Kinetics and Checkpoint Responses From RAD51-mediated Gene Conversion. Mol Cell Biol. 2005;25(3):933-44. PubMed PMID: 15657422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RAD51-dependent break-induced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion. AU - Malkova,Anna, AU - Naylor,Maria L, AU - Yamaguchi,Miyuki, AU - Ira,Grzegorz, AU - Haber,James E, PY - 2005/1/20/pubmed PY - 2005/3/10/medline PY - 2005/1/20/entrez SP - 933 EP - 44 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 25 IS - 3 N2 - Diploid Saccharomyces cells experiencing a double-strand break (DSB) on one homologous chromosome repair the break by RAD51-mediated gene conversion >98% of the time. However, when extensive homologous sequences are restricted to one side of the DSB, repair can occur by both RAD51-dependent and RAD51-independent break-induced replication (BIR) mechanisms. Here we characterize the kinetics and checkpoint dependence of RAD51-dependent BIR when the DSB is created within a chromosome. Gene conversion products appear within 2 h, and there is little, if any, induction of the DNA damage checkpoint; however, RAD51-dependent BIR occurs with a further delay of 2 to 4 h and cells arrest in response to the G(2)/M DNA damage checkpoint. RAD51-dependent BIR does not require special facilitating sequences that are required for a less efficient RAD51-independent process. RAD51-dependent BIR occurs efficiently in G(2)-arrested cells. Once repair is initiated, the rate of repair replication during BIR is comparable to that of normal DNA replication, as copying of >100 kb is completed less than 30 min after repair DNA synthesis is detected close to the DSB. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/15657422/RAD51_dependent_break_induced_replication_differs_in_kinetics_and_checkpoint_responses_from_RAD51_mediated_gene_conversion_ L2 - https://journals.asm.org/doi/10.1128/MCB.25.3.933-944.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -