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Identification of genes and proteins induced during the lag and early exponential phase of lager brewing yeasts.
J Appl Microbiol. 2005; 98(2):261-71.JA

Abstract

AIMS

The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth.

METHODS AND RESULTS

Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager brewing yeast in minimal medium. The identified, early-induced proteins were Ade17p, Eno2p, Ilv5gp, Sam1p, Rps21p and Ssa2p. For most of these proteins, the patterns of induction differed from those of the corresponding genes. However, the genes had similar early expression patterns in minimal medium as observed during lager brewing conditions. The expression of previously identified early-induced genes in Saccharomyces cerevisiae grown in minimal medium, ADO1, ALD6, ASC1, ERG4, GPP1, RPL25, SSB1 and YKL056C, was also early induced in lager yeast under brewing conditions.

CONCLUSIONS

The results indicate that the above-mentioned genes in general are induced during the lag phase and early exponential growth in Saccharomyces yeasts. The processes in which these genes take part are likely to play an important role during growth initiation.

SIGNIFICANCE AND IMPACT OF THE STUDY

Increased knowledge regarding the early growth phase of lager brewing yeast was obtained. Further, the universality of the identified expression patterns suggests new methodologies for optimization and control of growth initiation during brewing fermentations.

Authors+Show Affiliations

Brejning J
Department of Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Rolighedsvej, Frederiksberg C, Denmark.
Arneborg N
No affiliation info available
Jespersen L
No affiliation info available

MeSH

Electrophoresis, Gel, Two-DimensionalFermentationGene Expression Regulation, FungalGenes, FungalIndustrial MicrobiologySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15659180

Citation

Brejning, J, et al. "Identification of Genes and Proteins Induced During the Lag and Early Exponential Phase of Lager Brewing Yeasts." Journal of Applied Microbiology, vol. 98, no. 2, 2005, pp. 261-71.
Brejning J, Arneborg N, Jespersen L. Identification of genes and proteins induced during the lag and early exponential phase of lager brewing yeasts. J Appl Microbiol. 2005;98(2):261-71.
Brejning, J., Arneborg, N., & Jespersen, L. (2005). Identification of genes and proteins induced during the lag and early exponential phase of lager brewing yeasts. Journal of Applied Microbiology, 98(2), 261-71.
Brejning J, Arneborg N, Jespersen L. Identification of Genes and Proteins Induced During the Lag and Early Exponential Phase of Lager Brewing Yeasts. J Appl Microbiol. 2005;98(2):261-71. PubMed PMID: 15659180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of genes and proteins induced during the lag and early exponential phase of lager brewing yeasts. AU - Brejning,J, AU - Arneborg,N, AU - Jespersen,L, PY - 2005/1/22/pubmed PY - 2005/5/25/medline PY - 2005/1/22/entrez SP - 261 EP - 71 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 98 IS - 2 N2 - AIMS: The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS: Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager brewing yeast in minimal medium. The identified, early-induced proteins were Ade17p, Eno2p, Ilv5gp, Sam1p, Rps21p and Ssa2p. For most of these proteins, the patterns of induction differed from those of the corresponding genes. However, the genes had similar early expression patterns in minimal medium as observed during lager brewing conditions. The expression of previously identified early-induced genes in Saccharomyces cerevisiae grown in minimal medium, ADO1, ALD6, ASC1, ERG4, GPP1, RPL25, SSB1 and YKL056C, was also early induced in lager yeast under brewing conditions. CONCLUSIONS: The results indicate that the above-mentioned genes in general are induced during the lag phase and early exponential growth in Saccharomyces yeasts. The processes in which these genes take part are likely to play an important role during growth initiation. SIGNIFICANCE AND IMPACT OF THE STUDY: Increased knowledge regarding the early growth phase of lager brewing yeast was obtained. Further, the universality of the identified expression patterns suggests new methodologies for optimization and control of growth initiation during brewing fermentations. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/15659180/Identification_of_genes_and_proteins_induced_during_the_lag_and_early_exponential_phase_of_lager_brewing_yeasts_ DB - PRIME DP - Unbound Medicine ER -