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Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4-phosphate pathway.
Plant Cell. 2005 Feb; 17(2):628-43.PC

Abstract

The biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the two building blocks for isoprenoid biosynthesis, occurs by two independent pathways in plants. The mevalonic pathway operates in the cytoplasm, and the methyl-d-erythritol 4-phosphate (MEP) pathway operates in plastids. Plastidic isoprenoids play essential roles in plant growth and development. Plants must regulate the biosynthesis of isoprenoids to fulfill metabolic requirements in specific tissues and developmental conditions. The regulatory events that modulate the plant MEP pathway are not well understood. In this article, we demonstrate that the CHLOROPLAST BIOGENESIS6 (CLB6) gene, previously shown to be required for chloroplast development, encodes 1-hydroxy-2-methyl-butenyl 4-diphosphate reductase, the last-acting enzyme of the MEP pathway. Comparative analysis of the expression levels of all MEP pathway gene transcripts and proteins in the clb6-1 mutant background revealed that posttranscriptional control modulates the levels of different proteins in this central pathway. Posttranscriptional regulation was also found during seedling development and during fosmidomycin inhibition of the pathway. Our results show that the first enzyme of the pathway, 1-deoxy-d-xylulose 5-phosphate synthase, is feedback regulated in response to the interruption of the flow of metabolites through the MEP pathway.

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Authors+Show Affiliations

Guevara-García A
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos 62271, Mexico. aguevara@ibt.unam.mx
San Román C
No affiliation info available
Arroyo A
No affiliation info available
Cortés ME
No affiliation info available
de la Luz Gutiérrez-Nava M
No affiliation info available
León P
No affiliation info available

MeSH

Amino Acid SequenceArabidopsisArabidopsis ProteinsBase SequenceChloroplast ProteinsErythritolGene Expression ProfilingGene Expression Regulation, EnzymologicGene Expression Regulation, PlantGenetic Complementation TestMolecular Sequence DataMutationPhylogenyPlants, Genetically ModifiedSugar Phosphates

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15659625

Citation

Guevara-García, Arturo, et al. "Characterization of the Arabidopsis Clb6 Mutant Illustrates the Importance of Posttranscriptional Regulation of the methyl-D-erythritol 4-phosphate Pathway." The Plant Cell, vol. 17, no. 2, 2005, pp. 628-43.
Guevara-García A, San Román C, Arroyo A, et al. Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4-phosphate pathway. Plant Cell. 2005;17(2):628-43.
Guevara-García, A., San Román, C., Arroyo, A., Cortés, M. E., de la Luz Gutiérrez-Nava, M., & León, P. (2005). Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4-phosphate pathway. The Plant Cell, 17(2), 628-43.
Guevara-García A, et al. Characterization of the Arabidopsis Clb6 Mutant Illustrates the Importance of Posttranscriptional Regulation of the methyl-D-erythritol 4-phosphate Pathway. Plant Cell. 2005;17(2):628-43. PubMed PMID: 15659625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4-phosphate pathway. AU - Guevara-García,Arturo, AU - San Román,Carolina, AU - Arroyo,Analilia, AU - Cortés,María Elena, AU - de la Luz Gutiérrez-Nava,María, AU - León,Patricia, Y1 - 2005/01/19/ PY - 2005/1/22/pubmed PY - 2005/12/13/medline PY - 2005/1/22/entrez SP - 628 EP - 43 JF - The Plant cell JO - Plant Cell VL - 17 IS - 2 N2 - The biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the two building blocks for isoprenoid biosynthesis, occurs by two independent pathways in plants. The mevalonic pathway operates in the cytoplasm, and the methyl-d-erythritol 4-phosphate (MEP) pathway operates in plastids. Plastidic isoprenoids play essential roles in plant growth and development. Plants must regulate the biosynthesis of isoprenoids to fulfill metabolic requirements in specific tissues and developmental conditions. The regulatory events that modulate the plant MEP pathway are not well understood. In this article, we demonstrate that the CHLOROPLAST BIOGENESIS6 (CLB6) gene, previously shown to be required for chloroplast development, encodes 1-hydroxy-2-methyl-butenyl 4-diphosphate reductase, the last-acting enzyme of the MEP pathway. Comparative analysis of the expression levels of all MEP pathway gene transcripts and proteins in the clb6-1 mutant background revealed that posttranscriptional control modulates the levels of different proteins in this central pathway. Posttranscriptional regulation was also found during seedling development and during fosmidomycin inhibition of the pathway. Our results show that the first enzyme of the pathway, 1-deoxy-d-xylulose 5-phosphate synthase, is feedback regulated in response to the interruption of the flow of metabolites through the MEP pathway. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/15659625/Characterization_of_the_Arabidopsis_clb6_mutant_illustrates_the_importance_of_posttranscriptional_regulation_of_the_methyl_D_erythritol_4_phosphate_pathway_ DB - PRIME DP - Unbound Medicine ER -
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